Team:Groningen/Notebook/tasA Purification t76
Blue Bio Energy
tasA Purification (t76)
The digestion samples were purified to get rid of the restriction enzymes.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
tasA and Psalt digestion products were gel purified.
Gel purification
00:00, 17 August 2015 - 00:00, 17 August 2015
Correct fragments were cut out of the gel and purified with the PCR purification kit.