Team:Groningen/Notebook/tasA Sequencing t38
Blue Bio Energy
tasA sequencing (t38)
tasA was send for sequencing to Macrogen.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
Indeed the restriction sites in tasA are gone.
None
00:00, 18 June 2015 - 00:00, 18 June 2015
24TA 3, one of the tasA digestion samples, was send to Macrogen for sequencing:
5µL 1/20 primer (5 pmol/µL)
5µL plasmid DNA (24TA 3)
Sequencing code Macrogen:
Forward primer (5 tasA for): 1790ZAB067
Reverse primer (6 tasA rev): 1790ZAB068
00:00, 19 June 2015 - 00:00, 19 June 2015
The seqencing results showed the tasA insert without restriction sites. The restriction sites are gone.