Team:Groningen/Notebook/tasA Transformation t42
Blue Bio Energy
tasA Heat Shock Transformation (t42)
The ligation product of tasA in pSB1C3 was transformed into E. coli.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The heat shock transformation of tasA in pSB1C3 was successful.
Heat shock transformation
00:00, 8 July 2015 - 00:00, 8 July 2015
Afterwards a heat shock transformation was performed with 100 µL NEB competent cells + ligation product.
90% plate (90µL)
10% plate (10µL)
The plates were incubated overnight at 37 °C.
00:00, 9 July 2015 - 00:00, 9 July 2015
The transformation was checked. On both 10% and 90% plates a large amount of colonies had grown, so the transformation was successful. 4 colonies from the 10% plate were grown overnight in 3 mL LB+cm (6 µL 1/500).