Team:Groningen/Notebook/tasA Transformation t78
Blue Bio Energy
Transformation of tasA with Psalt (t78)
The ligation product of tasA with Psalt was transformed into E. coli.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
A heat shock transformation was performed to transform tasA with Psalt in E. coli.
Heat shock transformation
00:00, 17 August 2015 - 00:00, 17 August 2015
The ligations of tasA with the salt promoter were used to transform E. coli cells from NEB. 10 μL ligation mix was used for each transformation. The transformations were plated on LB plates with appropriate antibiotics. Ligations were performed according to manufacturer with an ratio 1:4 (Backbone:insert).