Introduction

1. 為何偵測此毒物

   餿水油中發現鉛的殘留，可能是因為由含有鉛的動物內臟製成，或是金屬容器的鉛溶出導致的污染。餿水油中含有鉛[1]，而鉛不易由人體代謝或降解，對人體是一種慢性和積累性毒物，容易導致中毒或甚至致癌等等，所以我們決定要偵測鉛。

2. 此毒物的危害

   Lead can cause several unwanted effects, such as:

   • Disruption of the biosynthesis of haemoglobin and anaemia
   • A rise in blood pressure
   • Kidney damage
   • Miscarriages and subtle abortions
   • Disruption of nervous systems
   • Brain damage
   • Declined fertility of men through sperm damage
   • Diminished learning abilities of children
   • Behavioural disruptions of children, such as aggression, impulsive behavior and hyperactivity

   Lead can enter a foetus through the placenta of the mother. Because of this it can cause serious damage to the nervous system and the brains of unborn children.[2]

3. 台灣法規

Circuit Design

We mainly divide the whole experiment into 2 major parts.

The former fragment can consistently produce PbRr.[4]

If the oil contains lead ions, they can integrate with proteins PbRr to further activate proteins.

As for the later fragment, the lead promoter can be controlled by activated protein PbRr to further trigger the GFP.

研究問題與結果

1. 此毒物能否進入ecoli
   1. Method
   2. Result
   3. Discussion
2. 大腸桿菌能否耐受此毒物
   1. Method
   2. Result
   3. Discussion
3. 某毒物濃度與螢光亮度的關係
   1. Method
   2. Result
   3. Discussion

Reference

• [1] Rancidity of Used Cooking Oil and Heavy Metal Analyses on Selected Street-Vended Foods (Annabelle A. Callano, 2012)
• [2] Health effects of lead (http://www.lenntech.com/periodic/elements/pb.htm)
• [3] 食用油脂類衛生標準，食品衛生管理法第十七條
• [4] An Exceptionally Selective Lead(ii)-Regulatory Protein from Ralstonia Metallidurans: Development of a Fluorescent Lead(ii) Probe (Peng Chen, Bill Greenberg, Safiyh Taghavi, Christine Romano, Daniel van der Lelie, and Chuan He)