Team:Korea U Seoul/Requirement/Medal criteria/content
1.Register for iGEM, have a great summer, and attend the Giant Jamboree.
We have registered for iGEM and developed our project since January, 2015. Especially, during the summer vacation, our team members learned the knowledge about python, advanced biology and how to create web app with visualization function. Also, we learned how to work in a team with many interactions. You can see the details in our notebook page in wiki. (Notebook page link) We will present our project in Giant Jamboree.
2.Complete the Judging form.
We have completed the Judging Form.
3.Create and share a Description of the team's project using the iGEM wiki, and document the team's parts (if any) using the Registry of Standard Biological Parts.
We have described our team’s project on iGEM wiki and documented our team’s parts.
4.Present a poster and a talk at the iGEM Jamboree. See the 2015 poster guidelines for more information.
We have prepared a fascinating poster for the iGEM Jamboree. If you have interest in our project, come and listen at the Giant Jamboree.
5.Create a page on your team wiki with clear attribution of each aspect of your project. This page must clearly attribute work done by the students and distinguish it from work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.
We have created attribution page that describes clear attribution of each aspect of our project. For more details, please visit attribution page.
6. Develop and make available, via the iGEM GitHub page, an open source software that supports Synthetic Biology based on Standard Parts or interacts with the Registry. (For questions about the iGEM Github page, contact software [at] igem [dot] org.)
You can access our software which is open source through GitHub. Our software supports Synthetic Biology with the biobrick of the enzyme gene sequences. More details, please visit our GitHub page.
The main objective of our project is to construct a novel “protein whip” platform, with which we can make Corynebacterium glutamicum to express other corynebacterium’s pili structure comprised of chains of a protein of our choice. As our first try, we decided to make pili made out of green fluorescence proteins (GFP); in order to do so, we substituted SpaA protein, one of the surface proteins in the Pilin A gene cluster, into green fluorescence protein, and transformed a vector containing the modified Pilin A gene cluster into a C. glutamicum strain.
Our “protein whip” platform is expected to have many practical applications. For example, pili made out of an enzyme, enzyme whip will enable the reaction to take place with high efficiency, for a great number of the enzyme included in the pili will be able to “attack” the reactants simultaneously. Biofilms made of strains of bacteria that express pili comprised of chains of specific amino acids such as histidine or cysteine that readily bind to heavy metals may be utilized to purify water contaminated with heavy metals.
Having a number of potential applications is not the sole merit of our project; by using C. glutamicum instead of widely exploited Escherichia coli, our project also contributes to expanding model organisms used in synthetic biology beyond E. coli.