Difference between revisions of "Team:LZU-China/chz index"

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             <h2 class="center">Abstract</h2>
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             <p class="light">With the global industrialization, heavy metal ions have become significant factors of water pollution, which may cause serious damage to human health. However, the devices to measure the concentration of heavy metals are complex and expensive. Therefore, the aim of our project was to create a cheaper and portable device. In our project, we have applied several transcription factors which are sensitive to the concentration of copper ions and chromium ions to regulate the expression of protein ribB. The existence of ribB will enhance the production of riboflavin. Riboflavin, as the electron carrier, improves the electricity producing performance of the Microbial Fuel Cells (MFCs). So in this way, we successfully correlated the concentration of heavy metal ions together with the output voltage of the MFC. This process is used within a convenient new device we created, Micro Holmes (a combination of MFC and electronic components), to monitor heavy metal ions in water sample any place any time.</p>
 
             <p class="light">With the global industrialization, heavy metal ions have become significant factors of water pollution, which may cause serious damage to human health. However, the devices to measure the concentration of heavy metals are complex and expensive. Therefore, the aim of our project was to create a cheaper and portable device. In our project, we have applied several transcription factors which are sensitive to the concentration of copper ions and chromium ions to regulate the expression of protein ribB. The existence of ribB will enhance the production of riboflavin. Riboflavin, as the electron carrier, improves the electricity producing performance of the Microbial Fuel Cells (MFCs). So in this way, we successfully correlated the concentration of heavy metal ions together with the output voltage of the MFC. This process is used within a convenient new device we created, Micro Holmes (a combination of MFC and electronic components), to monitor heavy metal ions in water sample any place any time.</p>
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                           <div class="collapsible-header"><i class="material-icons">place</i><p><strong>Collaborate with other teams</strong></p></div>
 
                           <div class="collapsible-header"><i class="material-icons">place</i><p><strong>Collaborate with other teams</strong></p></div>
                           <div class="collapsible-body"><p>Mentored Shiyan_SY_China<br/>Characterize parts for TJU & SZU<br/>Help modifying E.coli for TJU<br/>
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                           <div class="collapsible-body"><p>Mentored Shiyan_SY_China<a href="https://2015.igem.org/Team:LZU-China/Collaborations#SHI_SY_CHINA">More details</a><br/>Characterize parts for <a href="https://2015.igem.org/Team:LZU-China/Collaborations#TJU">TJU</a>  & <a href="https://2015.igem.org/Team:LZU-China/Collaborations#SZU">SZU</a><br/>Help modifying E.coli for TJU<br/>
 
                           <a href="https://2015.igem.org/Team:LZU-China/Collaborations">More details</a></p></div>
 
                           <a href="https://2015.igem.org/Team:LZU-China/Collaborations">More details</a></p></div>
 
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Revision as of 17:08, 18 September 2015

Team:LZU 2015



Micro Holmes

A Novel Device for Monitoring Heavy Metal Ions


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Abstract

With the global industrialization, heavy metal ions have become significant factors of water pollution, which may cause serious damage to human health. However, the devices to measure the concentration of heavy metals are complex and expensive. Therefore, the aim of our project was to create a cheaper and portable device. In our project, we have applied several transcription factors which are sensitive to the concentration of copper ions and chromium ions to regulate the expression of protein ribB. The existence of ribB will enhance the production of riboflavin. Riboflavin, as the electron carrier, improves the electricity producing performance of the Microbial Fuel Cells (MFCs). So in this way, we successfully correlated the concentration of heavy metal ions together with the output voltage of the MFC. This process is used within a convenient new device we created, Micro Holmes (a combination of MFC and electronic components), to monitor heavy metal ions in water sample any place any time.

Contents

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Human Practicemore_vert

For more details

Human Practiceclose

We've done an investigation about water pollution in the past few months. Through street interviews and online questionnaires,369 valid replies were collected from 9 countries. Here's the conclusion: Although different countries have varying degrees of water pollution, people are uniformly worried about this. Accordingly, there is support, and even demand for a method that is both cheap and convenient to tell them whether their water is safe to drink. And that's the reason for this project.

Wet Labmore_vert

For more details

Wet Labclose

We had constructed 14 different parts. All of them, including several ion sensors, have ribB CDS. Our experiments confirmed that the expression degree of ribB can influence the riboflavin production. Then we took them into MFC and the voltage showed that our genetically engineered E.coli responded to the change of Cu2+ concentration. Thus we linked the concentration of metal ion to the electricity production.

Dry Labmore_vert

For more details

Dry Labclose

Based on the wet lab results, we constructed the Micro Holmes concentration measurement system' which contained a Microbial Fuel Cell, a Microprogrammed Control Unit, a voltage magnification circuit board, an Analogue-to-Digital Conversion chip, a set of input and output devices and one or several kinds of bacteria.

Parts

Team

Collaborations

High Lgihts

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  • filter_drama

    1.The new application of ribB

    ribB protein is able to increase and stabilize electrical current generated by microbial fuel cells. Expression level of ribB is correlated with MFC voltage.

  • place

    2.Heavy metals sensors

    Copper, Mercury, zinc, chromium sensors were used to regulate ribB gene's expression in bio-engineered strains and concentrations of heavy metals are correlated with ribB protein production.

  • whatshot

    3.MFC voltage correlated with heavy metal ions

    When grown our bio-engineered strains in the anode of MFC, voltage is correlated with heavy metal ion concentration.

  • whatshot

    4.A novel device for heavy metal ion detection/measurement

    A novel device for heavy metal ion detection/measurement has been developed based on our findings. Comparing to current heavy metal measurement instruments, our device is simpler, cheaper and easier to use.

  • whatshot

    5.An innovative Human Practices activity

    Through street interview and online questionnaires, 369 valid replies were collected from 30 provinces in China, and 9 different foreign countries. What's more the innovative public education activity garnered a huge response.

 

Modal Fullfillment

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Bronze
Sliver
  • filter_drama

    Validate new parts

  • place

    Interlab Study

    We have participated the Interlab Study and documented the data.

  • whatshot

    Human Practise

    We have investigated and addressed the issues related to water safety especially about water pollution.
    More details

Gold
  • filter_drama

    Substantial improvement of measurement project

    We constructed the cheap and convenient device to measure the pollution.

  • place

    Accessibility and/or portability

    The accessibility and portability have been demonstrated by other teams.

  • whatshot

    Innovative Human Practices activity

    hrough street interview and online questionnaires, 369 valid replies were collected from 30 provinces in China, and 9 different foreign countries. What’s more the innovative public education activity garnered a huge response.More details

  • place

    Collaborate with other teams

    Mentored Shiyan_SY_ChinaMore details
    Characterize parts for TJU & SZU
    Help modifying E.coli for TJU
    More details