Difference between revisions of "Team:LZU-China/chz index"
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<h1 class="header center teal-text text-lighten-2">Micro Holmes</h1> | <h1 class="header center teal-text text-lighten-2">Micro Holmes</h1> | ||
<div class="row center"> | <div class="row center"> | ||
| − | <h5 class="header col s12 "> <span class="light-blue-text text- | + | <h5 class="header col s12 "> <span class="light-blue-text text-accent-4"> A Novel Device for Monitoring Heavy Metal Ions</span></h5> |
</div> | </div> | ||
<div class="row center"> | <div class="row center"> | ||
| − | <a href="http://7xlnim.com1.z0.glb.clouddn.com/ | + | <a href="http://7xlnim.com1.z0.glb.clouddn.com/igemshipin.mp4" class="btn-large waves-effect waves-light teal lighten-1">Watch video</a> |
</div> | </div> | ||
<br><br> | <br><br> | ||
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<h4 class="center">Abstract</h4> | <h4 class="center">Abstract</h4> | ||
| − | <p class="light">With the global industrialization, heavy metal ions have become significant factors of water pollution, which may cause serious damage to human health. However, the devices to measure the concentration of heavy metals are complex and expensive. Therefore, the aim of our project was to create a cheaper and portable device. In our project, we have applied several transcription factors which are sensitive to the concentration of copper ions and chromium ions to regulate the expression of protein ribB. | + | <p class="light">With the global industrialization, heavy metal ions have become significant factors of water pollution, which may cause serious damage to human health. However, the devices to measure the concentration of heavy metals are complex and expensive. Therefore, the aim of our project was to create a cheaper and portable device. In our project, we have applied several transcription factors which are sensitive to the concentration of copper ions and chromium ions to regulate the expression of protein ribB.<em>RibB</em> will enhance the production of riboflavin. Riboflavin, as the electron carrier, improves the electricity producing performance of the Microbial Fuel Cells (MFCs). So in this way, we successfully correlated the concentration of heavy metal ions together with the output voltage of the MFC. This process is used within a convenient new device we created, Micro Holmes (a combination of MFC and electronic components), to monitor heavy metal ions in water sample any place any time.</p> |
</div> | </div> | ||
</div> | </div> | ||
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</div> | </div> | ||
<div class="card-content"> | <div class="card-content"> | ||
| − | <span class="card-title activator grey-text text-darken-4">Human | + | <span class="card-title activator grey-text text-darken-4">Human Practices<i class="material-icons right">more_vert</i></span> |
<p><a href="https://2015.igem.org/Team:LZU-China/Practices"><span class="light-green-text text-darken-4"><strong>For more details</strong></span> </a></p> | <p><a href="https://2015.igem.org/Team:LZU-China/Practices"><span class="light-green-text text-darken-4"><strong>For more details</strong></span> </a></p> | ||
</div> | </div> | ||
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<span class="card-title grey-text text-darken-4">Human Practice<i class="material-icons right">close</i></span> | <span class="card-title grey-text text-darken-4">Human Practice<i class="material-icons right">close</i></span> | ||
<p>We've done an investigation about water pollution in the past few months. Through street interviews and online questionnaires,369 valid replies were collected from 9 countries. Here's the conclusion: Although different countries have varying degrees of water pollution, people are uniformly worried about this. Accordingly, there is support, and even demand for a method that is both cheap and convenient to tell them whether their water is safe to drink. And that's the reason for this project.</p> | <p>We've done an investigation about water pollution in the past few months. Through street interviews and online questionnaires,369 valid replies were collected from 9 countries. Here's the conclusion: Although different countries have varying degrees of water pollution, people are uniformly worried about this. Accordingly, there is support, and even demand for a method that is both cheap and convenient to tell them whether their water is safe to drink. And that's the reason for this project.</p> | ||
| + | <a href="https://2015.igem.org/Team:LZU-China/Practices"><span class="blue-text text-darken-2">more details</span></a> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="card-reveal"> | <div class="card-reveal"> | ||
<span class="card-title grey-text text-darken-4">Wet Lab<i class="material-icons right">close</i></span> | <span class="card-title grey-text text-darken-4">Wet Lab<i class="material-icons right">close</i></span> | ||
| − | <p>We had constructed 14 different parts. All of them, including several ion sensors, have ribB CDS. Our experiments confirmed that the expression degree of ribB can influence the riboflavin production. Then we took them into MFC and the voltage showed that our genetically engineered E.coli responded to the change of Cu<sup>2+</sup> concentration. Thus we linked the concentration of metal ion to the electricity production.</p> | + | <p>We had constructed 14 different parts. All of them, including several ion sensors, have <em>ribB</em> CDS. Our experiments confirmed that the expression degree of <em>ribB</em> can influence the riboflavin production. Then we took them into MFC and the voltage showed that our genetically engineered E.coli responded to the change of Cu<sup>2+</sup> concentration. Thus we linked the concentration of metal ion to the electricity production.</p> |
| + | <a href="https://2015.igem.org/Team:LZU-China/project#wetlab"><span class="blue-text text-darken-2">more details</span></a> | ||
</div> | </div> | ||
</div> | </div> | ||
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<span class="card-title grey-text text-darken-4">Dry Lab<i class="material-icons right">close</i></span> | <span class="card-title grey-text text-darken-4">Dry Lab<i class="material-icons right">close</i></span> | ||
<p>Based on the wet lab results, we constructed the Micro Holmes concentration measurement system' which contained a Microbial Fuel Cell, a Microprogrammed Control Unit, a voltage magnification circuit board, an Analogue-to-Digital Conversion chip, a set of input and output devices and one or several kinds of bacteria.</p> | <p>Based on the wet lab results, we constructed the Micro Holmes concentration measurement system' which contained a Microbial Fuel Cell, a Microprogrammed Control Unit, a voltage magnification circuit board, an Analogue-to-Digital Conversion chip, a set of input and output devices and one or several kinds of bacteria.</p> | ||
| + | <a href="https://2015.igem.org/Team:LZU-China/project#drylab"><span class="blue-text text-darken-2">more details</span></a> | ||
</div> | </div> | ||
</div> | </div> | ||
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<ul class="collapsible popout" data-collapsible="accordion"> | <ul class="collapsible popout" data-collapsible="accordion"> | ||
<li> | <li> | ||
| − | <div class="collapsible-header"><i class="material-icons"> | + | <div class="collapsible-header"><i class="material-icons">place</i> <p><h5>1.The new application of <em>ribB</em></h5></p></div> |
| − | <div class="collapsible-body"><p>ribB protein is able to increase and stabilize electrical current generated by microbial fuel cells. Expression level of ribB is correlated with MFC voltage.</p></div> | + | <div class="collapsible-body"><p>ribB protein is able to increase and stabilize electrical current generated by microbial fuel cells. Expression level of <em>ribB</em> is correlated with MFC voltage.</p></div> |
</li> | </li> | ||
<li> | <li> | ||
<div class="collapsible-header"><i class="material-icons">place</i><p><h5>2.Heavy metals sensors</h5></p></div> | <div class="collapsible-header"><i class="material-icons">place</i><p><h5>2.Heavy metals sensors</h5></p></div> | ||
| − | <div class="collapsible-body"><p>Copper, Mercury, zinc, chromium sensors were used to regulate ribB gene's expression in bio-engineered strains and concentrations of heavy metals are correlated with ribB protein production. </p></div> | + | <div class="collapsible-body"><p>Copper, Mercury, zinc, chromium sensors were used to regulate <em>ribB</em> gene's expression in bio-engineered strains and concentrations of heavy metals are correlated with <em>ribB</em> protein production. </p></div> |
</li> | </li> | ||
<li> | <li> | ||
| − | <div class="collapsible-header"><i class="material-icons"> | + | <div class="collapsible-header"><i class="material-icons">place</i><p><h5>3.MFC voltage correlated with heavy metal ions</h5></p></div> |
<div class="collapsible-body"><p>When grown our bio-engineered strains in the anode of MFC, voltage is correlated with heavy metal ion concentration.</p></div> | <div class="collapsible-body"><p>When grown our bio-engineered strains in the anode of MFC, voltage is correlated with heavy metal ion concentration.</p></div> | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <div class="collapsible-header"><i class="material-icons"> | + | <div class="collapsible-header"><i class="material-icons">place</i><p><h5>4.A novel device for heavy metal ion detection/measurement</h5></p> </div> |
<div class="collapsible-body"><p>A novel device for heavy metal ion detection/measurement has been developed based on our findings. Comparing to current heavy metal measurement instruments, our device is simpler, cheaper and easier to use.</p></div> | <div class="collapsible-body"><p>A novel device for heavy metal ion detection/measurement has been developed based on our findings. Comparing to current heavy metal measurement instruments, our device is simpler, cheaper and easier to use.</p></div> | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <div class="collapsible-header"><i class="material-icons"> | + | <div class="collapsible-header"><i class="material-icons">place</i><p><h5>5.An innovative Human Practices activity</h5></p></div> |
<div class="collapsible-body"><p>Through street interview and online questionnaires, 369 valid replies were collected from 30 provinces in China, and 9 different foreign countries. What's more the innovative public education activity garnered a huge response.</p></div> | <div class="collapsible-body"><p>Through street interview and online questionnaires, 369 valid replies were collected from 30 provinces in China, and 9 different foreign countries. What's more the innovative public education activity garnered a huge response.</p></div> | ||
</li> | </li> | ||
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</div> | </div> | ||
</div> | </div> | ||
| − | <div class="parallax"><img src=" | + | <div class="parallax"><img src="https://static.igem.org/mediawiki/2015/c/c9/LZU-China-2015-modal.png" alt="Unsplashed background img 3"></div> |
</div> | </div> | ||
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<ul class="collapsible popout" data-collapsible="accordion"> | <ul class="collapsible popout" data-collapsible="accordion"> | ||
<li> | <li> | ||
| − | <div class="collapsible-header"><i class="material-icons">done</i><p><strong> | + | <div class="collapsible-header"><i class="material-icons">done</i><p><strong>New parts</strong></p></div> |
<div class="collapsible-body"><p><a href="http://parts.igem.org/Part:BBa_K1755301">BBa_K1755301</a> <br/> | <div class="collapsible-body"><p><a href="http://parts.igem.org/Part:BBa_K1755301">BBa_K1755301</a> <br/> | ||
<a href="http://parts.igem.org/Part:BBa_K1755302">BBa_K1755302</a></p></div> | <a href="http://parts.igem.org/Part:BBa_K1755302">BBa_K1755302</a></p></div> | ||
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<ul class="collapsible popout" data-collapsible="accordion"> | <ul class="collapsible popout" data-collapsible="accordion"> | ||
<li> | <li> | ||
| − | <div class="collapsible-header"><i class="material-icons">done</i><p><strong> | + | <div class="collapsible-header"><i class="material-icons">done</i><p><strong>Improvement</strong></p></div> |
| − | <div class="collapsible-body"><p>We constructed the cheap and convenient device to measure the pollution.</p></div> | + | <div class="collapsible-body"><p>We constructed the cheap and convenient device to measure the pollution.</br> |
| + | <a href="https://2015.igem.org/Team:LZU-China/project#parts">More details</a></p> | ||
| + | </div> | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <div class="collapsible-header"><i class="material-icons">done</i><p><strong> | + | <div class="collapsible-header"><i class="material-icons">done</i><p><strong>Portability</strong></p></div> |
| − | <div class="collapsible-body"><p>The accessibility and portability have been demonstrated by other teams.</p></div> | + | <div class="collapsible-body"><p>The accessibility and portability have been demonstrated by other teams.</br> |
| + | <a href="https://2015.igem.org/Team:LZU-China/project#Accessibility and portability">More details</a></p> | ||
| + | </div> | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <div class="collapsible-header"><i class="material-icons">done</i><p><strong>Innovative | + | <div class="collapsible-header"><i class="material-icons">done</i><p><strong>Innovative H.P.</strong></p></div> |
<div class="collapsible-body"><p>hrough street interview and online questionnaires, 369 valid replies were collected from 30 provinces in China, and 9 different foreign countries. What’s more the innovative public education activity garnered a huge response.<a href="https://2015.igem.org/Team:LZU-China/Practices">More details</a></p></div> | <div class="collapsible-body"><p>hrough street interview and online questionnaires, 369 valid replies were collected from 30 provinces in China, and 9 different foreign countries. What’s more the innovative public education activity garnered a huge response.<a href="https://2015.igem.org/Team:LZU-China/Practices">More details</a></p></div> | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <div class="collapsible-header"><i class="material-icons">done</i><p><strong> | + | <div class="collapsible-header"><i class="material-icons">done</i><p><strong>Collaboration</strong></p></div> |
<div class="collapsible-body"><p>Mentored Shiyan_SY_China<a href="https://2015.igem.org/Team:LZU-China/Collaborations#SHI_SY_CHINA">More details</a><br/>Characterize parts for <a href="https://2015.igem.org/Team:LZU-China/Collaborations#attri">TJU</a> & <a href="https://2015.igem.org/Team:LZU-China/Collaborations#advis">SZU</a><br/>Help modifying E.coli for TJU<br/> | <div class="collapsible-body"><p>Mentored Shiyan_SY_China<a href="https://2015.igem.org/Team:LZU-China/Collaborations#SHI_SY_CHINA">More details</a><br/>Characterize parts for <a href="https://2015.igem.org/Team:LZU-China/Collaborations#attri">TJU</a> & <a href="https://2015.igem.org/Team:LZU-China/Collaborations#advis">SZU</a><br/>Help modifying E.coli for TJU<br/> | ||
<a href="https://2015.igem.org/Team:LZU-China/Collaborations">More details</a></p></div> | <a href="https://2015.igem.org/Team:LZU-China/Collaborations">More details</a></p></div> | ||
Latest revision as of 03:33, 19 September 2015
Abstract
With the global industrialization, heavy metal ions have become significant factors of water pollution, which may cause serious damage to human health. However, the devices to measure the concentration of heavy metals are complex and expensive. Therefore, the aim of our project was to create a cheaper and portable device. In our project, we have applied several transcription factors which are sensitive to the concentration of copper ions and chromium ions to regulate the expression of protein ribB.RibB will enhance the production of riboflavin. Riboflavin, as the electron carrier, improves the electricity producing performance of the Microbial Fuel Cells (MFCs). So in this way, we successfully correlated the concentration of heavy metal ions together with the output voltage of the MFC. This process is used within a convenient new device we created, Micro Holmes (a combination of MFC and electronic components), to monitor heavy metal ions in water sample any place any time.
Contents
We've done an investigation about water pollution in the past few months. Through street interviews and online questionnaires,369 valid replies were collected from 9 countries. Here's the conclusion: Although different countries have varying degrees of water pollution, people are uniformly worried about this. Accordingly, there is support, and even demand for a method that is both cheap and convenient to tell them whether their water is safe to drink. And that's the reason for this project.
more details
We had constructed 14 different parts. All of them, including several ion sensors, have ribB CDS. Our experiments confirmed that the expression degree of ribB can influence the riboflavin production. Then we took them into MFC and the voltage showed that our genetically engineered E.coli responded to the change of Cu2+ concentration. Thus we linked the concentration of metal ion to the electricity production.
more details
Based on the wet lab results, we constructed the Micro Holmes concentration measurement system' which contained a Microbial Fuel Cell, a Microprogrammed Control Unit, a voltage magnification circuit board, an Analogue-to-Digital Conversion chip, a set of input and output devices and one or several kinds of bacteria.
more details
Highlights
-
place
1.The new application of ribB
ribB protein is able to increase and stabilize electrical current generated by microbial fuel cells. Expression level of ribB is correlated with MFC voltage.
-
place
2.Heavy metals sensors
Copper, Mercury, zinc, chromium sensors were used to regulate ribB gene's expression in bio-engineered strains and concentrations of heavy metals are correlated with ribB protein production.
-
place
3.MFC voltage correlated with heavy metal ions
When grown our bio-engineered strains in the anode of MFC, voltage is correlated with heavy metal ion concentration.
-
place
4.A novel device for heavy metal ion detection/measurement
A novel device for heavy metal ion detection/measurement has been developed based on our findings. Comparing to current heavy metal measurement instruments, our device is simpler, cheaper and easier to use.
-
place
5.An innovative Human Practices activity
Through street interview and online questionnaires, 369 valid replies were collected from 30 provinces in China, and 9 different foreign countries. What's more the innovative public education activity garnered a huge response.
Medal Fullfillment
Bronze
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done
Register the team
The team LZU-China has been registered successfully.
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done
Judging form
Our Judging form
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done
Team wiki
You are reading our team wiki now.
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done
Poster and Talk
Our Poster will be exhibited in the Hynes Convention Center during the Giant Jamboree. And the presentation will be given in Room 312 Sep. 26, 2:00 pm
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done
Attribution page
Click here to see our Attribution page.
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done
Document parts
Sliver
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done
New parts
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done
Interlab Study
We have participated the Interlab Study and documented the data.
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done
Human Practices
We have investigated and addressed the issues related to water safety especially about water pollution.
More details
Gold
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done
Improvement
We constructed the cheap and convenient device to measure the pollution. More details
-
done
Portability
The accessibility and portability have been demonstrated by other teams. More details
-
done
Innovative H.P.
hrough street interview and online questionnaires, 369 valid replies were collected from 30 provinces in China, and 9 different foreign countries. What’s more the innovative public education activity garnered a huge response.More details
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done
Collaboration
Mentored Shiyan_SY_ChinaMore details
Characterize parts for TJU & SZU
Help modifying E.coli for TJU
More details