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| | <meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> | | <meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> |
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| − | <link href="https://2015.igem.org/Template:Team:LaVerne-Leos/style.css?action=raw&ctype=text/css" rel="stylesheet" type="text/css" media="all" /> | + | <link href="https://2015.igem.org/Template:Team:LaVerne-Leos/stylecss?action=raw&ctype=text/css" rel="stylesheet" type="text/css" media="all" /> |
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| | </style> | | </style> |
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| | <link href="https://2015.igem.org/Template:Team:LaVerne-Leos/PopupCSS?action=raw&ctype=text/css" rel="stylesheet" type="text/css"> | | <link href="https://2015.igem.org/Template:Team:LaVerne-Leos/PopupCSS?action=raw&ctype=text/css" rel="stylesheet" type="text/css"> |
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| | <li class="active"><a href="https://2015.igem.org/Team:LaVerne-Leos">Home</a></li> | | <li class="active"><a href="https://2015.igem.org/Team:LaVerne-Leos">Home</a></li> |
| | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Project">Project</a></li> | | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Project">Project</a></li> |
| | + | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Parts">Parts</a></li> |
| | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Notebook">Notebook</a></li> | | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Notebook">Notebook</a></li> |
| − | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/HumanPractices">Human Practices</a></li> | + | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Practices">Human Practices</a></li> |
| | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Team">Team</a></li> | | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Team">Team</a></li> |
| − | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Safety">Safety</a></li> | + | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Attributions"> Attributions </a></li> |
| | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Collaborations">Collaborations</a></li> | | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Collaborations">Collaborations</a></li> |
| | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Sponsors">Sponsors</a></li> | | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Sponsors">Sponsors</a></li> |
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| | <h4>Human <br>Practices</h4> | | <h4>Human <br>Practices</h4> |
| | </div> | | </div> |
| − | <a href="https://2015.igem.org/Team:LaVerne-Leos/ HumanPractices"><div class="desc"> | + | <a href="https://2015.igem.org/Team:LaVerne-Leos/ Practices"><div class="desc"> |
| | <p>Click <u>here</u> to see the implications of our project!</p> | | <p>Click <u>here</u> to see the implications of our project!</p> |
| | </div></a> | | </div></a> |
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| | <a href="https://2015.igem.org/Team:LaVerne-Leos/Notebook"><div class="desc"> | | <a href="https://2015.igem.org/Team:LaVerne-Leos/Notebook"><div class="desc"> |
| | <p>Click <u>here</u> to view our protocols and steps throughout the project!</p> | | <p>Click <u>here</u> to view our protocols and steps throughout the project!</p> |
| − | </div> | + | </div></a> |
| | </div> | | </div> |
| − | <p> Free fatty acids are biofuel precursors. We focused on using zeaxanthin to counter the toxic effects of increased free fatty acids in an altered Synechococcus elongatus 7942 strain. Zeaxanthin acts as an antioxidant, stabilizes the membrane, and is needed for electron transport chain function. To increase the concentration of zeaxanthin and its precursors, we introduced a circuit containing parts of the zeaxanthin synthesis pathway. The La Cañada subset of our team focused on tocopherol, a metabolite that has similar properties to zeaxanthin in cyanobacteria. Tocopherol acts as an antioxidant, protects the cell from lipid peroxidation, and enhances photosynthesis. A circuit was made with the gene p-hydroxyphenylpyruvate dioxygenase to catalyze the formation of homogenistic acid, the rate limiting step of tocopherol synthesis. In an attempt to further increase the production of fatty acids, both zeaxanthin and tocopherol circuits are dynamically regulated, utilizing a fatty acid-sensitive promoter-repressor system, pLR and FadR. </p>
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| | <div class="content-middle"> | | <div class="content-middle"> |
| | <h2><span>Our work</span></h2> | | <h2><span>Our work</span></h2> |
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| | <link rel="stylesheet" href="https://2015.igem.org/Template:Team:LaVerne-Leos/ColorBoxCSS?action=raw&ctype=text/css"> | | <link rel="stylesheet" href="https://2015.igem.org/Template:Team:LaVerne-Leos/ColorBoxCSS?action=raw&ctype=text/css"> |
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| | <!-- include colorbox --> | | <!-- include colorbox --> |
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| | <li><a href="http://www.mathworks.com/products/matlab/" target="_blank"><img src="https://static.igem.org/mediawiki/2015/3/30/LaVerne-Leos_client3.png" /></a></li> | | <li><a href="http://www.mathworks.com/products/matlab/" target="_blank"><img src="https://static.igem.org/mediawiki/2015/3/30/LaVerne-Leos_client3.png" /></a></li> |
| | <li><a href="https://www.idtdna.com/site" target="_blank"><img src="https://static.igem.org/mediawiki/2015/2/21/LaVerne-Leos_client4.png" /></a> | | <li><a href="https://www.idtdna.com/site" target="_blank"><img src="https://static.igem.org/mediawiki/2015/2/21/LaVerne-Leos_client4.png" /></a> |
| | + | <li><a href="http://www.agilent.com/home" target="_blank"><img src="https://static.igem.org/mediawiki/2015/d/d1/LaVerneLeos_client5.png" /></a> |
| | </ul> | | </ul> |
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| | }); | | }); |
| | </script> | | </script> |
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| | </div> | | </div> |
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| | <div class="col_1_of_3 span_1_of_3"> | | <div class="col_1_of_3 span_1_of_3"> |
| | <h3>Contact info</h3> | | <h3>Contact info</h3> |
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| | <li><a href="https://2015.igem.org/Team:LaVerne-Leos">Home</a></li> | | <li><a href="https://2015.igem.org/Team:LaVerne-Leos">Home</a></li> |
| | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Project">Project</a></li> | | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Project">Project</a></li> |
| − | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Notebook">Notebook</a></li> | + | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Parts">Parts</a></li> |
| − | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/ HumanPractices">Human Practices</a></li> | + | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Notebook">Notebook</a></li> |
| | + | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/ Practices">Human Practices</a></li> |
| | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Team">Team</a></li> | | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Team">Team</a></li> |
| − | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Sponsors">Sponsors</a></li> | + | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Attributions">Attributions</a></li> |
| | + | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Collaborations">Collaborations</a></li> |
| | + | <li><a href="https://2015.igem.org/Team:LaVerne-Leos/Sponsors">Sponsors</a></li> |
| | </ul> | | </ul> |
| | </div> | | </div> |
