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| − | <h3>Bronze.</h3><p>
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| − | 1. Register for iGEM, have a great summer, and attend the Giant Jamboree.<br><br>
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| − | 2. Complete the Judging form.<br><br>
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| − | 3. Create and share a Description of the team's project using the iGEM wiki, and document the team's parts using the Registry of Standard Biological Parts.<br><br>
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| − | 4. Present a poster and a talk at the iGEM Jamboree.<br><br>
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| − | 5. Create a page on your team wiki with clear attribution of each aspect of your project. This page must clearly attribute work done by the students and distinguish it from work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.</p>
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| − | <div id="green"><p>Attributions can be found in the “Attribution” tab on our wiki.</p></div><p><br><br>
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| − | 6. Document at least one new standard BioBrick Part or Device central to your project and submit this part to the iGEM Registry. You may also document a new application of a BioBrick part from a previous iGEM year, adding that documentation to the part's main page.</p>
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| − | <div id="green"><p>We submitted part BBa_K1843000, which is adapted for thrombin consisting of additional cut sites and a GFP reporter. The part submitted gives the many other teams inspired by the same publications, an easy control for in vitro translation reactions. Since it already contains a full switch and reporter, it can be transformed and purified and then used directly with a cell-free expression system, without any further cloning. The trigger is short enough to be quickly and cheaply bought as a DNA oligo. Thus, future teams exploring cell-free expression systems can test their systems induced with a DNA oligo (positive reaction control) and without (negative reaction control).</p></div><p><br><br><br>
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| − | <h3 class="silver">Silver:</h3><p>
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| − | 1. Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected. Document the characterization of this part in the Main Page section of the Registry entry for that Part/Device. This working part must be different from the part you documented in Bronze medal criterion #6.
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| − | <div id="green"><p>Experimentally, we showed that part BBa_K1843000 is a successful RNA toehold switch, which responds to a specific DNA trigger and expresses GFP, with low background.</p></div><p><br><br>
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| − | 2. Submit this new part to the iGEM Parts Registry. This part must be different from the part you documented in Bronze medal criterion #6.<br><br>
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| − | 3. iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, and intellectual property rights. We refer to these activities as Human Practices in iGEM. Demonstrate how your team has identified, investigated and addressed one or more of these issues in the context of your project.
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| − | <div id="green"><p>Aptapaper has potential applications as a rapid diagnostic tool, which could be beneficial in countries that specifically have problems with clean drinking water or a prevalence of infectious diseases. This is one issue we identified, and hence it is why we distributed a survey to individuals in Thailand and the Philippines to gauge interest in such a diagnostic tool. This survey ultimately provided us with an idea of how Aptapaper would have to be designed in order to meet the needs of people who could potentially be using it. For human practice, we also address the issues in current detection systems for common diseases such as HPV and malaria, and highlight how detection could be improved using our system. We also talked to representatives at Caymen Chemical and and the Kellogg Company to learn more about how Aptapaper could have applications in industry.</p></div><p><br><br><br>
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| − | <h3>Gold:</h3><p>
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| − | 1. Choose one of these two options: (1) Expand on your silver medal Human Practices activity by demonstrating how you have integrated the investigated issues into the design and/or execution of your project. OR (2) Demonstrate an innovative Human Practices activity that relates to your project
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| − | <div id="green"><p>We expanded on our human practice and helped it improve our design by using the information gained from our surveys. From the surveys, we learned that most people want a detection system that produces results in less than a day. When we designed our construct, we therefore developed a system that would not take long to reach maximum GFP expression. Specifically, we found from our results that detection takes within three hours, suggesting that our system would be well-received.</div></p><p><br><br>
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| − | 2. Help any registered iGEM team from a high-school, different track, another university, or institution in a significant way by, for example, mentoring a new team, characterizing a part, debugging a construct, modeling/simulating their system or helping validate a software/hardware solution to a synbio problem.
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| − | <div id="green"><p>We worked with the Heidelberg iGEM team this year, and they gave us advice on our project but suggesting a modified switch design with a trigger identical to one already used. Although we ran into experimental obstacles, we produced some promising results. We also skyped with rookie team, Nait_Edmonton, and helped them navigate iGEM policies and advised them on how to recruitment and team logistics. Additionally, we sent our laboratory protocols to the University of Michigan Software Team who designed a protocol database for their project.</div></p><p><br><br>
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| − | 3. Improve the function OR characterization of a previously existing BioBrick Part or Device (created by another team, or by your own team in in a previous year of iGEM), and enter this information in the part's page on the Registry.<br><br>
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| − | 4. Demonstrate a functional prototype of your project. Your prototype can derive from a previous project (that was not demonstrated to work) by your team or by another team. Show this system working under real-world conditions that you simulate in the lab.
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| − | <div id="green"><p>We showed that our system is functional because as shown in the graph below, GFP expression increases with an increasing concentration of thrombin.</div></p>
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