Results
The products that were cloned into the shipping vector were verified by gel electrophoresis (Figure 1).
Several colonies were identified in colony screens for PCSK1 and insulin in transformants containing the shipping vector. Seen below are verification gels for PCSK1 and insulin.
Figure 1. PCR colony screen of PCSK1-pSB1C3 transformants. Note the banding in each lane at roughly 750 bp, consistent with the second fragment of PCSK1.
Figure 2. PCR colony screen of Ins-pSB1C3 transformants. Banding can be seen at roughly 333 bp, consistent with the full insulin ORF.
Sequencing data received for both PCSK1 and insulin was inconclusive.
Parts List
BBa_K1187001 Human insulin, codon optimized for expression in P. pastoris
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