Figure 1. PCR colony screen of PCSK1-pSB1C3 transformants. Note the banding in each lane at roughly 750 bp, consistent with the second fragment of PCSK1.
Figure 2. PCR colony screen of Ins-pSB1C3 transformants. Banding can be seen at roughly 333 bp, consistent with the full insulin ORF.
Sequencing data received for both PCSK1 and insulin was inconclusive.
Parts List
BBa_K1187001 Human insulin, codon optimized for expression in P. pastoris
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