Team:NCTU Formosa/Acheivement

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Acheivement

• Cotramsforming Biobricks for creating Customized Detection Platform
• We built three type of biobrick libraries: scFv , color signal and GBP to display on E.coli’s outer membrane for creating customized detection platform.
• With cotransformation, we offered several of customized combinations, such as E.Cotector with different scFv of targeted drugs and different color signal or with GBP.
• We proved that the binding specificity of E.Cotectors by our cell staining experiment and that suggest the scFv have successfully displayed on the surface of E.coli.
• Our E.Cotector succeeded in displaying GBP on the surface and binding on gold chip.

• We constructed 25 biobricks and conducted a series of experiments to verify their functions.
• We designed the mechanism to guarantee the safety on the gene level and the microorganism level.
• To make our safety mechanism be more complete, we found out the optimal way to process and the best condition for conserving fluoresce proteins by modeling.
• With our modeling, we realized how E.Cotector would grow and the condition of protein displaying.
• Consulting with law expert to realize the law we should notice for our E.Cotector.
• We introduced our ideas to doctors and asked some questions for us to realize problems and values of our project.
• We introduced our ideas to Biotech company- ApexBio and asked some questions for us to realize problems and values of our project.
• We designed a survey for public to recognize the pre-diagnosis of targeted drugs treatment.
• We held “Be bald” activity for public to know more about the crucial of the pre-diagnosis of cancer treatments and also introduced iGEM with them during the activity.
• Visiting the recovery cancer patient made us more realize the patient’s realistic circumstance and the importance of health.
• HSNU iGEM team and Mingdo iGEM team was helped by us to continue commitment to their Pro
• We held iGEM Asia Conference for 27 teams to meetup, exchange ideas, make friends and cooperate mutually.
• We promoted the communication and the cooperation among various rofessional fields such as synthetic biology, measurement technology, immunology, and medical science via the participation in iGEM and the accomplishment of the project.

• Simultaneously detected the multiple antigens.
• For more quantitative data and more application on precise measurements, we testified the function of GBP to bind on gold with the concept of Biosensor.
• Enhanced the process yield in immobilization of antibodies on the medium gold surface.
• The cost of using our E.cotectors is lower than using monoclonal antibodies targeted drugs directly on measuring antigens.

• Offer a brand new direct method for doctors to determining the usage of monoclonal-antibody-targeted drugs.
• Directly used the monoclonal-antibody-targeted-drugs to detect the corresponding antigen on the cancer cells.
• Provided a direct, personalized and customized pre-diagnosis of targeted drugs treatment detection platform.
• Offer the prescription of combination therapy to doctors.

Golden Medal

Expand on your silver medal Human Practices activity by demonstrating how you have integrated the investigated issues into the design and/or execution of your project.

Help any registered iGEM team from a high-school, different track, another university, or institution in a significant way.

Improve the function OR characterization of a previously existing BioBrick Part or Device (created by another team, or by your own team in in a previous year of iGEM), and enter this information in the part's page on the Registry.

Silver Medal

Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected. Document the characterization in the Main Page section of the Registry.

Submit this new part to the iGEM Parts Registry.

Demonstrate how your team has identified, investigated and addressed one or more of Human Practices in the context of your project.

Bronze Medal

Register for iGEM, have a great summer, and attend the Giant Jamboree.

Complete the Judging form.

Create and share a Description of the team's project using the iGEM wiki, and document the team's parts using the Registry of Standard Biological Parts.

Present a poster and a talk at the iGEM Jamboree.

Create a page on your team wiki with clear attribution of each aspect of your project.

Document at least one new standard BioBrick Part or Device central to your project and submit this part to the iGEM Registry.

Complete the Judging form.