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Revision as of 05:41, 14 September 2015
Lab note
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15 Transformation of GBP[FadL-GBP] Cultivation of OmpA[OmpA+pSB1K3] Kr |
16 Cultivation of GBP[FadL-GBP] Plasmid purification of OmpA[OmpA+pSB1K3] Kr Digestion of OmpA[OmpA+pSB1K3] Kr (EcoRI and PstI) |
17 Plasmid purification of GBP[FadL-GBP] Digestion of GBP[FadL-GBP] (EcoRI and PstI) Electrophoresis of digested product: GBP[FadL-GBP] (EcoRI and PstI) |
18 Transformation of ligated product: GBP[FadL-GBP+pSB1C3] Cr |
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19 Cultivation of ligated product: GBP[FadL-GBP] Cr PCR product: Terminator[J61048] |
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21 Cultivation of GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar OmpA[OmpA+pSB1C3] Cr GBP[FadL-GBP+pSB1C3] Cr |
22 Plasmid purification of GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar OmpA[OmpA+pSB1C3] Cr GBP[FadL-GBP+PSB1C3] Cr Digestion of GFP[J23101+B0034+E0040+J61048] Ar (EcoRI and PstI) OmpA[OmpA] Cr (EcoRI and PstI) GBP[FadL-GBP] Cr (EcoRI and PstI) Electrophoresis of digested product: GFP[J23101+B0034+E0040+J61048] Ar (EcoRI and PstI) OmpA[OmpA] Cr (EcoRI and PstI) GBP[FadL-GBP] Cr (EcoRI and PstI) |
23 Digestion of OmpA[OmpA] Cr (EcoRI and SpeI) GBP[FadL-GBP] Cr (EcoRI and SpeI) Electrophoresis of digested product: OmpA[OmpA] Cr (EcoRI and SpeI) GBP[FadL-GBP] Cr (EcoRI and SpeI) |
24 Digestion of OmpA[OmpA] Cr (XbaI and PstI) GBP[FadL-GBP] Cr (XbaI and PstI) |
25 Electrophoresis of digested product: OmpA[OmpA] Cr (XbaI and PstI) GBP[FadL-GBP] Cr (XbaI and PstI) Digestion of RBS[B0034] Ar (XbaI and PstI) backbone[pSB1A3] Ar (EcoRI and PstI) Terminator[J61048] Ar (XbaI and PstI) Electrophoresis of digested product: RBS[B0034] Ar (XbaI and PstI) backbone[pSB1A3] Ar (EcoRI and PstI) Terminator[J61048] Ar (XbaI and PstI) Ligation of OmpA[J23101+B0034+OmpA+pSB1A3] Ar GBP[GBP+B0034+pSB1K3] Kr OmpA[OmpA+J61048+pSB1K3] Kr |
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26 Ligation of RFP[E1010+B0034+pSB1K3] Kr Transformation of ligated product: OmpA[J23101+B0034+OmpA+pSB1A3] Ar GBP[FadL-GBP+B0034+pSB1K3] Kr OmpA[OmpA+J61048+pSB1K3] Kr Electrophoresis of digested product: RFP[E1010+J61048+pSB1K3] Kr [J23101+B0034] |
27 Digestion of Terminator[J61048] Ar (XbaI and PstI) PCR of ligated product: OmpA[J23101+B0034+OmpA+pSB1A3] Ar OmpA[OmpA+J61048+pSB1K3] Kr |
28 Electrophoresis of RFP[E1010+J61048+pSB1K3] Kr OmpA[J23101+B0034+OmpA+pSB1A3] Ar Transformation of ligated product: GBP[B0034+FadL-GBP+pSB1A3] Ar OmpA[OmpA+J61048+pSB1K3] Kr PCR of ligated product OmpA[J23101+B0034+OmpA] Ar |
29 Cultivation of RFP[E1010+J61048+pSB1K3] Kr PCR of OmpA[J23101+B0034+OmpA] Ar Electrophoresis of OmpA[J23101+B0034+OmpA] Ar Ligation of GBP[FadL-GBP+J61048+pSB1K3] Kr |
30 Plasmid purification of RFP[E1010+J61048+pSB1K3] Kr Ligation of OmpA[J23101+B0034+OmpA] Ar PCR of OmpA[J23101+B0034+OmpA] Ar Transformation of mini: Terminator[J61048] ligated product: OmpA[J23101+B0034+OmpA] Ar |
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NCTU_Formosa APOllO
National Chaio Tung University
Engineering Building 6 EF455, 1001 University Road, Hsinchu 300, Taiwan, ROC.