Difference between revisions of "Team:NEFU China/Parts"

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<h2> Part Documentation</h2>
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<div class="s2_1box" style="background:none;color:white;">
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<p><span style="font-family:arial,helvetica,sans-serif">Our parts are designed for the construction of the AI-2 response pathway in the engineered bacteria. The following Biobrick parts have been submitted to the registry:</span></p>
  
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>&nbsp;</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<p><span style="font-family:arial,helvetica,sans-serif"><span style="font-size:16px"><a href="http://parts.igem.org/Part:BBa_K1666000"><strong>BBa_K1666000</strong></a></span>: This is a coding region part for <em>lsrA</em>. LsrA is a part of the ABC transporter complex involved in autoinducer 2 (AI-2) import and responsible for energy coupling to the transport system.</span></p>
  
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<p><span style="font-family:arial,helvetica,sans-serif"><img alt="" src="https://static.igem.org/mediawiki/2015/d/df/NEFU_China_5B1371AF-71A4-4E89-B352-93158333F5CD.png" style="height:402px; width:330px" /></span></p>
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<h4>Note</h4>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<p><span style="font-family:arial,helvetica,sans-serif"><span style="font-size:16px"><a href="http://parts.igem.org/Part:BBa_K1666001"><strong>BBa_K1666001</strong></a>:</span> This is a coding region part for<em> lsrB</em>. LsrB is a solute-binding protein that can bind AI-2 specifically and help to transport the AI-2 into the cytoplasm.</span></p>
  
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<p><img alt="" src="https://static.igem.org/mediawiki/2015/8/89/NEFU_China_F3BC87FE-2FF1-4B8D-B2AA-B4B804EC42AE.png" style="height:386px; width:400px" /></p>
  
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<h4>Adding parts to the registry</h4>
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<p><span style="font-family:arial,helvetica,sans-serif"><span style="font-size:16px"><a href="http://parts.igem.org/Part:BBa_K1666002"><strong>BBa_K1666002</strong></a><strong> </strong></span><span style="font-size:14px">and<a href="http://parts.igem.org/Part:BBa_K1666003"><strong> </strong></a></span><span style="font-size:16px"><a href="http://parts.igem.org/Part:BBa_K1666003"><strong>BBa_K1666003</strong></a><strong>:</strong></span> LsrC and LsrD are parts of the ABC transporter complex LsrABCD, probably responsible for the translocation of the substrate across the membrane.</span></p>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<p><span style="font-family:arial,helvetica,sans-serif"><img alt="" src="https://static.igem.org/mediawiki/2015/2/23/NEFU_China_271EEC2F-42F6-4BEC-90BE-F9AABC9D19DB.png" style="height:377px; width:400px" />&nbsp;<img alt="" src="https://static.igem.org/mediawiki/2015/a/aa/NEFU_China_AAEA494B-1CA9-4C7D-B450-196DDFE12106.png" style="height:358px; width:400px" /></span></p>
  
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<h4>What information do I need to start putting my parts on the Registry?</h4>
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<p><span style="font-family:arial,helvetica,sans-serif"><span style="font-size:16px"><strong><a href="http://parts.igem.org/Part:BBa_K1666004">BBa_K1666004</a>:</strong> </span>This is a coding region part for <em>lsrK</em>. LsrK catalyzes the phosphorylation of autoinducer 2(AI-2) to phospho-AI-2, which subsequently inactivates the transcriptional regulator LsrR and leads to the transcription of the<em> lsr </em>operon.</span></p>
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<p>The information needed to initially create a part on the Registry is:</p>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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<p><span style="font-family:arial,helvetica,sans-serif"><img alt="" src="https://static.igem.org/mediawiki/2015/8/88/NEFU_China_9E5E499A-3565-4826-8C15-141D9D565A02.png" style="height:397px; width:320px" /></span></p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<p><span style="font-family:arial,helvetica,sans-serif"><span style="font-size:16px"><a href="http://parts.igem.org/Part:BBa_K1666005"><strong>BBa_K1666005</strong></a><strong>:</strong></span> This is a coding region part for <em>lsrR</em>. In the absence of autoinducer 2 (AI-2), LsrR represses transcription of the<em> lsr </em>operon and itself. Phospho-AI-2 can bind LsrR and inactivate it through releasing it from the repressed promoters, leading to the transcription of the<em> lsr</em> genes.</span></p>
  
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<p><span style="font-family:arial,helvetica,sans-serif"><img alt="" src="https://static.igem.org/mediawiki/2015/d/dd/NEFU_China_1F3FB0C1-1582-453D-9899-2A38F7F8DB60.png" style="height:361px; width:400px" /></span></p>
  
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<p><span style="font-family:arial,helvetica,sans-serif"><span style="font-size:16px"><a href="http://parts.igem.org/Part:BBa_K1666006"><strong>BBa_K1666006</strong></a>: </span>This is an inducible promoter. Plsr is the promoter of the <em>lsr </em>operon. It is under the repressive regulation of LsrR. In our project, we use it to regulate the expression of the reporter gene, <em>amilCP</em> (<a href="http://parts.igem.org/Part:BBa_K592009">BBa_K592009</a>).</span></p>
  
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<p><img alt="" src="https://static.igem.org/mediawiki/2015/f/f2/NEFU_China_B32F5186-DC8D-4430-A0C9-69AA75A37B79.png" style="height:342px; width:400px" /></p>
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<p class="footer" style="color:white;margin-top:20px;text-align:center;"><span>Generated by </span><a href="https://2015.igem.org/Team:NEFU_China/Software">Flight iGEM</a>.</p>
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<h4>Inspiration</h4>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<div class="fixl" style="width:300px">
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<table width="100%" border="0" cellspacing="0" cellpadding="0">
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h4>Part Table </h4>
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<groupparts>iGEM015 Example</groupparts>
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Latest revision as of 18:40, 18 September 2015

Our parts are designed for the construction of the AI-2 response pathway in the engineered bacteria. The following Biobrick parts have been submitted to the registry:

 

BBa_K1666000: This is a coding region part for lsrA. LsrA is a part of the ABC transporter complex involved in autoinducer 2 (AI-2) import and responsible for energy coupling to the transport system.

BBa_K1666001: This is a coding region part for lsrB. LsrB is a solute-binding protein that can bind AI-2 specifically and help to transport the AI-2 into the cytoplasm.

BBa_K1666002 and BBa_K1666003: LsrC and LsrD are parts of the ABC transporter complex LsrABCD, probably responsible for the translocation of the substrate across the membrane.

 

BBa_K1666004: This is a coding region part for lsrK. LsrK catalyzes the phosphorylation of autoinducer 2(AI-2) to phospho-AI-2, which subsequently inactivates the transcriptional regulator LsrR and leads to the transcription of the lsr operon.

BBa_K1666005: This is a coding region part for lsrR. In the absence of autoinducer 2 (AI-2), LsrR represses transcription of the lsr operon and itself. Phospho-AI-2 can bind LsrR and inactivate it through releasing it from the repressed promoters, leading to the transcription of the lsr genes.

BBa_K1666006: This is an inducible promoter. Plsr is the promoter of the lsr operon. It is under the repressive regulation of LsrR. In our project, we use it to regulate the expression of the reporter gene, amilCP (BBa_K592009).

Generated by Flight iGEM.

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