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| − | <li ><a href="/Team:NRP-UEA-Norwich/Team">Team</a></li>
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| − | <li class="dropdown " id="dropdown"><a href="#" class="dropdown-toggle" data-toggle="dropdown">Project</a>
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| − | <li ><a href="/Team:NRP-UEA-Norwich/Project">Background</a></li>
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| − | <li ><a href="/Team:NRP-UEA-Norwich/Approach">Approach</a></li>
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| − | <li ><a href="/Team:NRP-UEA-Norwich/Results">Results</a></li>
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| − | <li ><a href="/Team:NRP-UEA-Norwich/Parts">Parts</a></li>
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| − | <li ><a href="/Team:NRP-UEA-Norwich/Protocols">Protocols</a></li>
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| − | <li ><a href="/Team:NRP-UEA-Norwich/Modeling/Glyco2D">Glyco2D</a></li>
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| − | <li ><a href="/Team:NRP-UEA-Norwich/Modeling/3D">3D Models</a></li>
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| − | <li ><a href="/Team:NRP-UEA-Norwich/Modeling/Kinetic">Kinetic Model</a></li>
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| − | <li ><a href="/Team:NRP-UEA-Norwich/Modeling/Simulation">Acylation Simulation</a></li>
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| − | <li ><a href="/Team:NRP-UEA-Norwich/Attributions">Attributions</a></li>
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| − | <li ><a href="/Team:NRP-UEA-Norwich/Collaborations">Collaborations</a></li>
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| − | <h2 class="title1 f900" id ="top">RESULTS - PROBIOTIC</h2>
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| − | <li role="presentation" class="aqua scroll-link" data-id="top"><a href="#home1" aria-controls="home1" role="tab" data-toggle="tab"><span>- This is a really long title of a page</span></a></li>
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| − | <li role="presentation" class="aqua space80 scroll-link" data-id="top"><a href="#profile" aria-controls="profile" role="tab" data-toggle="tab"><span>- Title of page</span></a></li>
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| − | <li role="presentation" class="aqua scroll-link" data-id="top"><a href="#messages" aria-controls="messages" role="tab" data-toggle="tab"><span>- Title of page</span></a></li>
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| − | <li role="presentation" class="aqua scroll-link" data-id="top"><a href="#settings" aria-controls="settings" role="tab" data-toggle="tab"><span>- Title of page</span></a></li>
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| − | <li role="presentation" class="aqua scroll-link" data-id="top"><a href="#setting2" aria-controls="content2" role="tab" data-toggle="tab"><span>- Title of page</span></a></li>
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| − | <li role="presentation" class="aqua scroll-link" data-id="top"><a href="#setting3" aria-controls="content3" role="tab" data-toggle="tab"><span>- Title of page</span></a></li>
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| − | <li role="presentation" class="aqua scroll-link" data-id="top"><a href="#setting4" aria-controls="content4" role="tab" data-toggle="tab"><span>- Cloning of Plant Constructs</span></a></li>
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| − | <li role="presentation" class="aqua scroll-link space50" data-id="top"><a href="#setting5" aria-controls="content4" role="tab" data-toggle="tab"><span>- Title of page</span></a></li>
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| − | <h2 class="title1">Title Here</h2>
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| − | <p class="fsize20">Maybe a Date Here?</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − | <p class="space20">This new property means that silicon-based light detectors identify spin, so more information can be.</p>
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| − | <img src="https://static.igem.org/mediawiki/2015/5/58/NRP-UEA-Norwich-glyco2dbackground.png" href="https://static.igem.org/mediawiki/2015/5/58/NRP-UEA-Norwich-glyco2dbackground.png" alt="..." class="img-responsive mautomargin fancybox" style="cursor: pointer;">
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| − | <p><b>Figure 1:</b> A model structure of glycogen with acyl groups added.</p>
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − | <div class="col-md-3 col-sm-4 col-xs-6 space10"><img class="img-responsive fancybox" src="http://2.bp.blogspot.com/-H6MAoWN-UIE/TuRwLbHRSWI/AAAAAAAABBk/89iiEulVsyg/s400/Free%2BNature%2BPhoto.jpg" href="http://2.bp.blogspot.com/-H6MAoWN-UIE/TuRwLbHRSWI/AAAAAAAABBk/89iiEulVsyg/s400/Free%2BNature%2BPhoto.jpg" style="cursor:pointer;"></div>
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| − | <div class="col-md-3 col-sm-4 col-xs-6 space10"><img class="img-responsive fancybox" src="http://2.bp.blogspot.com/-H6MAoWN-UIE/TuRwLbHRSWI/AAAAAAAABBk/89iiEulVsyg/s400/Free%2BNature%2BPhoto.jpg" href="http://2.bp.blogspot.com/-H6MAoWN-UIE/TuRwLbHRSWI/AAAAAAAABBk/89iiEulVsyg/s400/Free%2BNature%2BPhoto.jpg" style="cursor:pointer;"></div>
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| − | <div class="col-md-3 col-sm-4 col-xs-6 space10"><img class="img-responsive fancybox" src="http://2.bp.blogspot.com/-H6MAoWN-UIE/TuRwLbHRSWI/AAAAAAAABBk/89iiEulVsyg/s400/Free%2BNature%2BPhoto.jpg" href="http://2.bp.blogspot.com/-H6MAoWN-UIE/TuRwLbHRSWI/AAAAAAAABBk/89iiEulVsyg/s400/Free%2BNature%2BPhoto.jpg" style="cursor:pointer;"></div>
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| − | <div class="col-md-3 col-sm-4 col-xs-6 space10"><img class="img-responsive fancybox" src="http://2.bp.blogspot.com/-H6MAoWN-UIE/TuRwLbHRSWI/AAAAAAAABBk/89iiEulVsyg/s400/Free%2BNature%2BPhoto.jpg" href="http://2.bp.blogspot.com/-H6MAoWN-UIE/TuRwLbHRSWI/AAAAAAAABBk/89iiEulVsyg/s400/Free%2BNature%2BPhoto.jpg" style="cursor:pointer;"></div>
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| − | <div class="col-md-3 col-sm-4 col-xs-6 space10"><img class="img-responsive fancybox" src="http://2.bp.blogspot.com/-H6MAoWN-UIE/TuRwLbHRSWI/AAAAAAAABBk/89iiEulVsyg/s400/Free%2BNature%2BPhoto.jpg" href="http://2.bp.blogspot.com/-H6MAoWN-UIE/TuRwLbHRSWI/AAAAAAAABBk/89iiEulVsyg/s400/Free%2BNature%2BPhoto.jpg" style="cursor:pointer;"></div>
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| − | <div class="col-md-3 col-sm-4 col-xs-6 space10"><img class="img-responsive fancybox" src="http://2.bp.blogspot.com/-H6MAoWN-UIE/TuRwLbHRSWI/AAAAAAAABBk/89iiEulVsyg/s400/Free%2BNature%2BPhoto.jpg" href="http://2.bp.blogspot.com/-H6MAoWN-UIE/TuRwLbHRSWI/AAAAAAAABBk/89iiEulVsyg/s400/Free%2BNature%2BPhoto.jpg" style="cursor:pointer;"></div>
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| − | <h2 class="title1">Title Here2</h2>
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| − | <p class="fsize20">Maybe a Date Here?</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space20">This new property means that silicon-based light detectors identify spin, so more information can be.</p>
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| − | <div class="col-md-12">
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − | <h2 class="title1">Title Here3</h2>
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| − | <p class="fsize20">Maybe a Date Here?</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space20">This new property means that silicon-based light detectors identify spin, so more information can be.</p>
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| − | <div class="col-md-6 space80">
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| − | <div class="col-md-12">
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − | <h2 class="title1">Title Here4</h2>
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| − | <p class="fsize20">Maybe a Date Here?</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space20">This new property means that silicon-based light detectors identify spin, so more information can be.</p>
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − | <h2 class="title1">Title Here 5</h2>
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| − | <p class="fsize20">Maybe a Date Here?</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space20">This new property means that silicon-based light detectors identify spin, so more information can be.</p>
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| − | </div>
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| − | <div class="col-md-6 space80">
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| − | <img src="image/Section-1-Photo.png" alt="..." class="img-responsive mautomargin">
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| − | <div class="col-md-12">
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − | <h2 class="title1">Title Here 6</h2>
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| − | <p class="fsize20">Maybe a Date Here?</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space20">This new property means that silicon-based light detectors identify spin, so more information can be.</p>
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − | <h2 class="title1">Making constructs to express putative acyl-transferases in plant chloroplasts</h2>
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| − | <h4 class="title2">AIM:</h4>
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| − | <p class="space10">The aim of our prebiotic is to produce acylated/butrylated starch in plants. Methods to chemically acylate starch purified from plants already exist, but as they use strong chemicals and require heating they are not environmentally friendly. Using various acyltransferases, we hope to acylate starch in plants. We’ll be using a model plant, <i>Nicotinia benthamiana</i>, for initial tests because we can get results within a few days. Later on we’d aim to make transgenic plants in a species makes a lot of starch such as maize (corn), potatoes or wheat.</p>
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| − | <h4 class="title2">METHOD:</h4>
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| − | <p class="space10">We used Golden Gate Cloning and the Plant Standard Syntax described in RFC 106 and Patron et al (2015) to make our constructs. We used 35s promoter from Cauliflower Mosiac Virus (BBa_K1467101), to drive constitutive expression. We made a chloroplast transit peptide (New part - BBa_K1618028) in an N-terminal translation fusion with the acyl transferases for the protein to reach the chloroplast, where starch is produced.
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| − | </p>
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| − |
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| − | <p class="space30">This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − | <div class="space30" id="ref"></div>
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| − | <h2 class="title1" id="ref">References</h2>
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| − |
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| − | <p >1. Meléndez-Hevia E., Waddell T.G., and Shelton E.D., 1993, <i> Optimization of molecular design in the evolution of metabolism: The glycogen molecule </i>, Biochem Journal, 295, p. 477–83</p>
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| − | <p><a name="2"></a>2. Meléndez R, Meléndez-Hevia E, Mas F, Mach J, Cascante M: <i>Physical constraints in the synthesis of glycogen that influence its structural homogeneity: A two-dimensional approach</i>. Biophys J 1998, 75:106–14.</p>
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| − | <p><a name="3"></a>3. Moreno-Bruna B, Baroja-Fernández E, Muñoz FJ, Bastarrica-Berasategui A, Zandueta-Criado A, Rodriguez-López M, Lasa I, Akazawa T, Pozueta-Romero J (2001) <i>Adenosine diphosphate sugar pyrophosphatase prevents glycogen biosynthesis in Escherichia coli. Proceedings of the National Academy of Sciences USA 98:8128–32</i>.</p>
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| − | <p><a name="4"></a>4. Blennow A, Nielsen TH, Baunsgaard L, Mikkelsen R, Engelsen SB (2002) <i>Starch phosphorylation: A new front line in starch research</i>. Trends in Plant Science 7:445–50</p>
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| − | <p><a name="5"></a>5. Bajka BH, Clarke JM, Topping DL, Cobiac L, Abeywardena MY, Patten G (2010) <i>Butyrylated starch increases large bowel butyrate levels and lowers colonic smooth muscle contractility in rats.</i> Nutrition Research 30:427–34</p>
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| − | <p><a name="6"></a>6. Wilson WA, Roach PJ, Montero M, Baroja-Fernández E, Muñoz FJ, Eydallin G, Viale AM, Pozueta-Romero J (2010) <i>Regulation of glycogen metabolism in yeast and bacteria.</i> FEMS Microbiology Reviews 34:952–85.</p>
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| − | <h2 class="title1">Title Here 8</h2>
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| − | <p class="fsize20">Maybe a Date Here?</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space20">This new property means that silicon-based light detectors identify spin, so more information can be.</p>
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| − | <div class="col-md-6 space80">
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| − | <img src="image/Section-1-Photo.png" alt="..." class="img-responsive mautomargin">
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| − | <div class="row">
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| − | <div class="col-md-12">
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − | <h2 class="title1">FLAV</h2>
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| − | <p class="fsize20">Maybe a Date Here?</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space20">This new property means that silicon-based light detectors identify spin, so more information can be.</p>
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| − | <div class="col-md-6 space80">
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| − | <img src="image/Section-1-Photo.png" alt="..." class="img-responsive mautomargin">
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| − |
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| − | <div class="row">
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| − | <div class="col-md-12">
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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| − |
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| − | <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
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