Difference between revisions of "Team:NTNU Trondheim/Protocols"
| Line 20: | Line 20: | ||
<li id="weekC" onclick="weekFilter(this)"> | <li id="weekC" onclick="weekFilter(this)"> | ||
<a class="nb-week" href="#C">March</a> | <a class="nb-week" href="#C">March</a> | ||
| − | </li | + | </li> |
| − | < | + | <li id="weekD" onclick="weekFilter(this)"> |
| + | <a class="nb-week" href="#D">April</a> | ||
| + | </li> | ||
| + | <li id="weekE" onclick="weekFilter(this)"> | ||
<a class="nb-week" href="#E">May</a> | <a class="nb-week" href="#E">May</a> | ||
</li--> | </li--> | ||
<li class="nb-month"> | <li class="nb-month"> | ||
| − | <div style="top: | + | <div style="top:55px">Media</div> |
</li> | </li> | ||
| − | <li id=" | + | <li id="week1" onclick="weekFilter(this)"> |
| − | <a class="nb-week" href="# | + | <a class="nb-week" href="#1">Lysogeny Broth (LB)</a> |
</li> | </li> | ||
| − | <li id=" | + | <li id="week2" onclick="weekFilter(this)"> |
| − | <a class="nb-week" href="# | + | <a class="nb-week" href="#2">SOC medium</a> |
</li> | </li> | ||
| − | <li id=" | + | <li id="week3" onclick="weekFilter(this)"> |
| − | <a class="nb-week" href="# | + | <a class="nb-week" href="#3">yB medium</a> |
</li> | </li> | ||
| − | <li id=" | + | <li id="week4" onclick="weekFilter(this)"> |
| − | <a class="nb-week" href="# | + | <a class="nb-week" href="#4"><i>Synechocystis</i> medium</a> |
| + | </li> | ||
| + | <li id="week5" onclick="weekFilter(this)"> | ||
| + | <a class="nb-week" href="#5"></a> | ||
</li> | </li> | ||
<li class="nb-month"> | <li class="nb-month"> | ||
| − | <div style="top: | + | <div style="top:140px;margin-left:-18px">Techniques</div> |
</li> | </li> | ||
| − | <li id=" | + | <li id="week6" onclick="weekFilter(this)"> |
| − | <a class="nb-week" href="# | + | <a class="nb-week" href="#6">Gibson assembly</a> |
</li> | </li> | ||
| − | <li id=" | + | <li id="week7" onclick="weekFilter(this)"> |
| − | <a class="nb-week" href="# | + | <a class="nb-week" href="#7">DNA isolation and cleaning</a> |
</li> | </li> | ||
| − | <li id=" | + | <li id="week8" onclick="weekFilter(this)"> |
| − | <a class="nb-week" href="# | + | <a class="nb-week" href="#8">DNA digestion</a> |
</li> | </li> | ||
| − | <li id=" | + | <li id="week9" onclick="weekFilter(this)"> |
| − | <a class="nb-week" href="# | + | <a class="nb-week" href="#9">PCR</a> |
</li> | </li> | ||
| − | <li id=" | + | <li id="week10" onclick="weekFilter(this)"> |
| − | <a class="nb-week" href="# | + | <a class="nb-week" href="#10">NanoDrop</a> |
</li> | </li> | ||
| − | <li | + | <li id="week11" onclick="weekFilter(this)"> |
| − | < | + | <a class="nb-week" href="#11">3A assembly</a> |
</li> | </li> | ||
| − | <li id=" | + | <li id="week12" onclick="weekFilter(this)"> |
| − | <a class="nb-week" href="# | + | <a class="nb-week" href="#12">Ligation</a> |
</li> | </li> | ||
| − | <li id=" | + | <li id="week13" onclick="weekFilter(this)"> |
| − | <a class="nb-week" href="# | + | <a class="nb-week" href="#13">Transformation (<i>Escherichia coli</i>)</a> |
</li> | </li> | ||
| − | <li id=" | + | <li id="week14" onclick="weekFilter(this)"> |
| − | <a class="nb-week" href="# | + | <a class="nb-week" href="#14">Transformation (<i>Synechocystis sp. PCC 6803</i>)</a> |
| − | + | ||
| − | + | ||
| − | + | ||
</li> | </li> | ||
| + | |||
<li class="nb-month"> | <li class="nb-month"> | ||
| − | <div style="top:52px;margin-left:- | + | <div style="top:52px;margin-left:-9px">Plasmids</div> |
</li> | </li> | ||
| − | <li id=" | + | <li id="week16" onclick="weekFilter(this)"> |
| − | <a class="nb-week" href="# | + | <a class="nb-week" href="#16">Right flank</a> |
</li> | </li> | ||
| − | <li id=" | + | <li id="week17" onclick="weekFilter(this)"> |
| − | <a class="nb-week" href="# | + | <a class="nb-week" href="#17">Left flank</a> |
</li> | </li> | ||
| − | <li id=" | + | <li id="week18" onclick="weekFilter(this)"> |
| − | <a class="nb-week" href="# | + | <a class="nb-week" href="#18">Kanamycin resistance</a> |
</li> | </li> | ||
| − | <li id=" | + | <li id="week19" onclick="weekFilter(this)"> |
| − | <a class="nb-week" href="# | + | <a class="nb-week" href="#19">Lac promoter</a> |
| + | </li> | ||
| + | <li id="week20" onclick="weekFilter(this)"> | ||
| + | <a class="nb-week" href="#20">Glucose oxidase</a> | ||
| + | </li> | ||
| + | <li id="week21" onclick="weekFilter(this)"> | ||
| + | <a class="nb-week" href="#21">Left flank + Kan + Right flank</a> | ||
| + | </li> | ||
| + | <li class="nb-month"> | ||
| + | |||
| + | <div style="top:55px;margin-left:-29px">Calculations</div> | ||
| + | </li> | ||
| + | <li id="week24" onclick="weekFilter(this)"> | ||
| + | <a class="nb-week" href="#24">DNA concentration</a> | ||
</li> | </li> | ||
</ul> | </ul> | ||
Revision as of 21:20, 23 June 2015
Jump to:
-
Media
- Lysogeny Broth (LB)
- SOC medium
- yB medium
- Synechocystis medium
-
Techniques
- Gibson assembly
- DNA isolation and cleaning
- DNA digestion
- PCR
- NanoDrop
- 3A assembly
- Ligation
- Transformation (Escherichia coli)
- Transformation (Synechocystis sp. PCC 6803)
-
Plasmids
- Right flank
- Left flank
- Kanamycin resistance
- Lac promoter
- Glucose oxidase
- Left flank + Kan + Right flank
-
Calculations
- DNA concentration
Protocols
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