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| − | {{NTNU Trondheim}}
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| − | <html>
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| − | <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:NTNU_Trondheim/Notebook/css/app?action=raw&ctype=text/css">
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| − | <body>
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| − |
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| − | <!-- ===================Month Filters=================== -->
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| − | <li>
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| − | <h6>Jump to:</h6>
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| − | </li>
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| − | <li class="nb-month">
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| − | <div style="top:55px">
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| − | Media
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| − | </div>
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| − | </li>
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| − |
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| − | <li id="week1" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#1">Lysogeny Broth (LB)</a>
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| − | </li>
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| − | <li id="week2" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#2">SOC medium</a>
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| − | </li>
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| − | <li id="week3" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#3">yB medium</a>
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| − | </li>
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| − | <li id="week4" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#4"><i>Synechocystis</i> medium</a>
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| − | </li>
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| − | <li id="week5" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#5"></a>
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| − | </li>
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| − | <li class="nb-month">
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| − | <div style="top:140px;margin-left:-18px">
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| − | Techniques
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| − | </div>
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| − | </li>
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| − | <li id="week6" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#6">Gibson assembly</a>
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| − | </li>
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| − | <li id="week7" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#7">DNA isolation and cleaning</a>
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| − | </li>
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| − | <li id="week8" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#8">DNA digestion</a>
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| − | </li>
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| − | <li id="week9" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#9">PCR</a>
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| − | </li>
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| − | <li id="week10" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#10">NanoDrop</a>
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| − | </li>
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| − | <li id="week11" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#11">3A assembly</a>
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| − | </li>
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| − | <li id="week12" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#12">Ligation</a>
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| − | </li>
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| − | <li id="week13" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#13">Transformation (<i>Escherichia coli</i>)</a>
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| − | </li>
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| − | <li id="week14" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#14">Transformation (<i>Synechocystis sp. PCC 6803</i>)</a>
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| − | </li>
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| − | <li class="nb-month">
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| − | <div style="top:52px;margin-left:-9px">
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| − | Plasmids
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| − | </div>
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| − | </li>
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| − | <li id="week16" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#16">Right flank</a>
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| − | </li>
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| − | <li id="week17" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#17">Left flank</a>
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| − | </li>
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| − | <li id="week18" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#18">Kanamycin resistance</a>
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| − | </li>
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| − | <li id="week19" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#19">Lac promoter</a>
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| − | </li>
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| − | <li id="week20" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#20">Glucose oxidase</a>
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| − | </li>
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| − | <li id="week21" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#21">Left flank + Kan + Right flank</a>
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| − | </li>
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| − | <li class="nb-month">
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| − | <div style="top:55px;margin-left:-29px">
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| − | Calculations
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| − | </div>
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| − | </li>
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| − | <li id="week24" onclick="weekFilter(this)">
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| − | <a class="nb-week" href="#24">DNA concentration</a>
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| − | </li>
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| − | </ul>
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| − | </div>
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| − |
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| − | <div class="ten columns team-bios-container">
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| − | <div class="row" id="ugd-members">
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| − | <div class="twelve columns">
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| − | <h2 class="centered">Protocols</h2>
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| − | </div>
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| − | </div>
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| − | <!-- ===================Subteam Filters=================== -->
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| − |
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| − |
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| − | <div class="row last-ele" style="padding-bottom:0px">
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| − | <h4 style="margin-left:15px;text-align:left">Filter by subteam:</h4>
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| − |
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| − |
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| − | <div class="nb-all" id="nb-all" onclick="showAll()">
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| − | <h4 style="margin-left:15px;text-align:left">show all categories</h4>
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| − | <div class="nb-all-i" id="nb-alltech" onclick="showAllTech()" style="margin-right:15px">
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| − | <h4 style="margin-left:15px;text-align:left">show technical details</h4>
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| − | </div>
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| − | </div>
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| − | </div>
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| − | <div class="row last-ele nb-labels" style="padding-bottom:0px">
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| − | <div style="opacity:0.00;width:65px">
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| − | "_"
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| − | </div>
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| − | <div>
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| − | Media
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| − | </div>
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| − | <div>
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| − | Techniques
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| − | </div>
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| − |
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| − | <div>
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| − | Plasmids
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| − | </div>
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| − |
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| − | <div>
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| − | Calculations
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| − | </div>
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| − | </div>
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| − | <div class="row">
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| − | <!-- PLACEHOLDER -->
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| − | <div class="two columns nb-filter" style="opacity:0.00;width:65px">
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| − | <div class="two columns nb-filter">
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| − | <div class="filter-container" onclick="filter(this)" style="background-image:url( https://static.igem.org/mediawiki/2015/5/5b/NTNU_Trondheim_media_off.png);height=100px;width=100px"><img height="100px" id="media" src="https://static.igem.org/mediawiki/2015/6/6b/NTNU_Trondheim_media_on.png" width="100px">
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| − | </div>
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| − | <div class="nb-only" id="media-only" onclick="onlyFilter(this)">
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| − | only
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| − | </div>
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| − | </div>
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| − | <div class="two columns nb-filter">
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| − | <div class="filter-container" onclick="filter(this)" style="background-image:url(https://static.igem.org/mediawiki/2015/1/19/NTNU_Trondheim_techniques_off.png);height=100px;width=100px"><img height="100px" id="techniques" src="https://static.igem.org/mediawiki/2015/b/b2/NTNU_Trondheim_techniques_on.png" width="100px">
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| − | </div>
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| − |
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| − |
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| − | <div class="nb-only" id="techniques-only" onclick="onlyFilter(this)">
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| − | only
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − | <div class="two columns nb-filter">
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| − | <div class="filter-container" onclick="filter(this)" style="background-image:url(https://static.igem.org/mediawiki/2015/6/66/NTNU_Trondheim_plasmids_off.png)"><img id="plas" src="https://static.igem.org/mediawiki/2015/7/74/NTNU_Trondheim_plasmids_on.png">
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| − | </div>
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| − |
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| − |
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| − | <div class="nb-only" id="plas-only" onclick="onlyFilter(this)">
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| − | only
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − | <div class="two columns nb-filter">
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| − | <div class="filter-container" onclick="filter(this)" style="background-image:url(https://2015.igem.org/File:NTNU_Trondheim_calculations_off.png);width=100px;height=100px"><img height="100px" id="calc" src="https://static.igem.org/mediawiki/2015/4/41/NTNU_Trondheim_calculations_on.png">
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| − | </div>
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| − |
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| − |
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| − | <div class="nb-only" id="calc-only" onclick="onlyFilter(this)">
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| − | only
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| − | </div>
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| − | </div>
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| − | <!-- PLACEHOLDER -->
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| − |
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| − |
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| − | <div class="two columns nb-filter" style="opacity:0.00;width:65px">
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| − | </div>
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| − | </div>
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| − | <!-- ===================Protocol entries=================== -->
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| − |
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| − |
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| − | <div class="nb-week" id="week1entry" style="display: block">
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| − | <div class="twelve columns">
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| − | <h3 class="centered">Lysogeny Broth (LB)</h3>
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| − |
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| − |
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| − | <p>
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| − | </p>
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| − |
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| − |
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| − | <div class="entry pc-media">
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| − | <div>
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| − | <h6>Recipe</h6>
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| − |
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| − |
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| − | <div class="nb-onetech-i nohilite">
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| − | <h6>Antibiotic additions</h6>
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| − | </div>
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| − |
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| − |
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| − | <div class="nb-tech">
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| − | <div class="AB-table">
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| − | <table>
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| − | <tr>
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| − | <th>Antibiotic</th>
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| − |
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| − | <th>Stock concentration</th>
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| − |
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| − | <th>Final concentration</th>
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| − |
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| − | <th>Dillution factor</th>
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| − |
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| − | <th>Solvent</th>
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| − |
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| − | <th>Storage temperature</th>
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| − | </tr>
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| − |
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| − | <tr>
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| − | <td>Ampicillin</td>
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| − | <td>50 mg / mL</td>
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| − |
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| − | <td>50 μg / mL</td>
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| − |
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| − | <td>1000</td>
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| − |
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| − | <td>Filter sterilized H<sub>2</sub>O</td>
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| − |
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| − | <td>4 °C</td>
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| − | </tr>
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| − |
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| − |
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| − | <tr>
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| − | <td>Chloramphenicol</td>
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| − |
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| − | <td>30 mg / mL</td>
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| − |
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| − | <td>30 μg / mL</td>
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| − |
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| − | <td>1000</td>
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| − |
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| − | <td>Ethanol</td>
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| − | <td>-20 °C</td>
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| − | </tr>
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| − |
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| − |
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| − | <tr>
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| − | <td>Kanamycin</td>
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| − |
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| − | <td>50 mg / mL</td>
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| − |
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| − | <td>30 μg / mL</td>
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| − |
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| − | <td>1000</td>
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| − |
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| − | <td>Filter sterilized H<sub>2</sub>O</td>
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| − | <td>4 °C</td>
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| − | </tr>
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| − |
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| − |
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| − | <tr>
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| − | <td>Spectinomycin</td>
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| − | <td>50 mg / mL</td>
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| − |
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| − | <td>50 μg / mL</td>
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| − |
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| − | <td>1000</td>
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| − |
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| − | <td>Filter sterilized H<sub>2</sub>O</td>
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| − |
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| − | <td>4 °C</td>
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| − | </tr>
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| − | </table>
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − | <p>Ingredients:<br></p>
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| − |
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| − |
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| − | <ul>
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| − | <li>Tryptone (10g)</li>
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| − |
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| − |
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| − | <li>NaCl (10g)</li>
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| − |
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| − |
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| − | <li>Yeast Extract (5g)</li>
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| − | </ul>
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| − | <br>
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| − |
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| − |
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| − | <p>
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| − | </p>
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| − |
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| − |
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| − | <ol>
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| − | <li>Fill with 1 L of distilled / filtered H<sub>2</sub>O.</li>
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| − |
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| − |
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| − | <li>Autoclave at 121 °C for 20 minutes.</li>
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| − |
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| − |
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| − | <li>Add antibiotics if needed, after the medium has cooled down.</li>
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| − | </ol>
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| − |
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| − |
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| − | <p>
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| − | </p>
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| − |
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| − |
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| − | <p>
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| − | </p>
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − | <p>
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| − | </p>
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − | <div class="nb-week" id="week2entry" style="display: block">
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| − | <div class="twelve columns">
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| − | <h3 class="centered">SOC medium</h3>
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| − |
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| − |
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| − | <p>
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| − | </p>
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| − |
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| − |
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| − | <div class="entry pc-media">
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| − | <div>
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| − | <h6>Recipe</h6>
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| − |
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| − |
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| − | <div class="nb-onetech-i nohilite">
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| − | <h6>show technical details</h6>
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| − | </div>
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| − |
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| − |
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| − | <div class="nb-tech">
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| − | {{{tech}}}
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| − | </div>
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| − |
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| − |
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| − | <p>
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| − | </p>
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| − |
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| − |
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| − | <p>Ingredients (1L):<br></p>
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| − |
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| − |
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| − | <ul>
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| − | <li>Bactotryptone (20 g)</li>
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| − |
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| − |
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| − | <li>Yeast extract (5g)</li>
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| − |
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| − |
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| − | <li>NaCl (0.584g)</li>
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| − |
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| − |
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| − | <li>KCl (0.186g)</li>
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| − |
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| − |
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| − | <li>Agar (20g ONLY IF MAKING PLATES)</li>
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| − | </ul>
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| − | <br>
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| − |
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| − |
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| − | <p>
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| − | </p>
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| − |
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| − |
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| − | <ol>
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| − | <li>Fill with 0.5 L of distilled / filtered H<sub>2</sub>O.</li>
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| − |
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| − |
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| − | <li>Autoclave at 121 °C for 20 minutes.</li>
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| − |
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| − |
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| − | <li>Add 10 mL 1M MgCl<sub>2</sub>, 10 mL MgSO<sub>4</sub> and 20 mL 1M glucose (all should be sterile) prior to use</li>
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| − | </ol>
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| − |
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| − |
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| − | <p>
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| − | </p>
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| − |
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| − |
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| − | <p>
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| − | </p>
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| − | </div>
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| − | </div>
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − | <div class="nb-week" id="week3entry" style="display: block">
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| − | <div class="twelve columns">
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| − | <h3 class="centered">yB medium</h3>
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| − |
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| − |
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| − | <p>
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| − | </p>
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| − |
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| − |
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| − | <div class="entry pc-media">
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| − | <div>
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| − | <h6>Recipe</h6>
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| − |
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| − |
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| − | <div class="nb-tech">
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| − | {{{tech}}}
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| − | </div>
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| − |
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| − |
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| − | <p>Ingredients:<br></p>
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| − |
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| − |
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| − | <ul>
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| − | <li>Yeast extract (2.5g)</li>
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| − |
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| − |
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| − | <li>Bactotryptone (10g)</li>
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| − |
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| − |
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| − | <li>KCL (0.38g)</li>
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| − | </ul>
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| − | <br>
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| − |
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| − |
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| − | <p>
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| − | </p>
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| − |
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| − |
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| − | <ol>
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| − | <li>Fill with 0.5 L of distilled / filtered H<sub>2</sub>O.</li>
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| − |
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| − |
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| − | <li>Add KOH until the pH is 7.4, then autoclave at 121 °C for 20 minutes.</li>
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| − |
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| − |
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| − | <li>Add 17 mL sterile 1M MgSO4</li>
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| − | </ol>
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| − |
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| − |
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| − | <p>
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| − | </p>
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| − |
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| − |
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| − | <p>
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| − | </p>
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| − | </div>
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| − | </div>
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − | <div class="nb-week" id="week4entry" style="display: block">
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| − | <div class="twelve columns">
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| − | <h3 class="centered">Synechocystis medium</h3>
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| − |
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| − |
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| − | <p>
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| − | </p>
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| − |
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| − |
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| − | <div class="entry pc-media">
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| − | <div>
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| − | <h6>Recipe</h6>
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| − |
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| − |
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| − | <div class="nb-tech">
| |
| − | {{{tech}}}
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| − | </div>
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| − |
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| − |
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| − | <p>To grow Synechocystis cultures, BG-11 medium was made according to the recipe found on the <a href="http://unitedscientists.org/labs/norway/NTNU/PhotoSynLab/wiki/table-of-contents-2/protocol-physiological/bg-11-media">PhotoSynLab wiki</a>.</p>
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| − | </div>
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| − | </div>
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − | <div class="nb-week" id="week6entry" style="display: block">
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| − | <div class="twelve columns">
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| − | <h3 class="centered">Gibson Assembly</h3>
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| − |
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| − |
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| − | <div class="entry pc-techniques">
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| − | <div>
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| − | <h6>
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| − | </h6>
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| − |
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| − |
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| − | <div class="nb-tech">
| |
| − | {{{tech}}}
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| − | </div>
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| − |
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| − |
| |
| − | <p>The Gibson assembly protocol can be found at <a href="https://www.neb.com/protocols/2012/12/11/gibson-assembly-protocol-e5510">New England Biolabs</a>.</p>
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| − | </div>
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| − | </div>
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − | <div class="nb-week" id="week7entry" style="display: block">
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| − | <div class="twelve columns">
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| − | <h3 class="centered">DNA isolation and cleaning</h3>
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| − |
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| − |
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| − | <p>
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| − | </p>
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| − |
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| − |
| |
| − | <div class="entry pc-techniques">
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| − | <div>
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| − | <div class="nb-tech">
| |
| − | {{{tech}}}
| |
| − | </div>
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| − |
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| − |
| |
| − | <p>For plasmid isolation, the Promega Wizard Plus SV Minipreps DNA Purification System A1460 Miniprep protocol was used. For PCR product isolation, the QIAquick PCR Purification kit was used.</p>
| |
| − | </div>
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| − | </div>
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| − | </div>
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| − | </div>
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| − |
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| − |
| |
| − | <div class="nb-week" id="week8entry" style="display: block">
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| − | <div class="twelve columns">
| |
| − | <h3 class="centered">DNA digestion</h3>
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| − |
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| − |
| |
| − | <p>
| |
| − | </p>
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| − |
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| − |
| |
| − | <div class="entry pc-techniques">
| |
| − | <div>
| |
| − | <div class="nb-tech">
| |
| − | {{{tech}}}
| |
| − | </div>
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| − |
| |
| − |
| |
| − | <p>DNA digests for both ligation and verification used the protocol in the <a href="http://unitedscientists.org/labs/norway/NTNU/PhotoSynLab/wiki/table-of-contents-2/protocol-molecular/dna-ligation/restriction-enzyme-digest">PhotoSynLab wiki</a>.</p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
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| − |
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| − |
| |
| − | <div class="nb-week" id="week9entry" style="display: block">
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| − | <div class="twelve columns">
| |
| − | <h3 class="centered">PCR</h3>
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| − |
| |
| − |
| |
| − | <p>
| |
| − | </p>
| |
| − |
| |
| − |
| |
| − | <div class="entry pc-techniques">
| |
| − | <div>
| |
| − | <div class="nb-tech">
| |
| − | {{{tech}}}
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <p>The touchdown PCR procedure detailed on the <a href="http://unitedscientists.org/labs/norway/NTNU/PhotoSynLab/wiki/table-of-contents-2/protocol-molecular/pcr-touchdown-pcr">PhotoSynLab wiki</a> was used to amplify DNA.</p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
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| − |
| |
| − |
| |
| − | <div class="nb-week" id="week10entry" style="display: block">
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| − | <div class="twelve columns">
| |
| − | <h3 class="centered">Nanodrop</h3>
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| − |
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| − |
| |
| − | <p>
| |
| − | </p>
| |
| − |
| |
| − |
| |
| − | <div class="entry pc-techniques">
| |
| − | <div>
| |
| − | <div class="nb-tech">
| |
| − | {{{tech}}}
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <p><a href="http://www.nanodrop.com/library/nd-1000-v3.7-users-manual-8.5x11.pdf">A NanoDrop ND-1000 Spectrophotometer</a> was used to determine DNA concentrations.</p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <div class="nb-week" id="week11entry" style="display: block">
| |
| − | <div class="twelve columns">
| |
| − | <h3 class="centered">3A assembly</h3>
| |
| − |
| |
| − |
| |
| − | <p>
| |
| − | </p>
| |
| − |
| |
| − |
| |
| − | <div class="entry pc-techniques">
| |
| − | <div>
| |
| − | <div class="nb-tech">
| |
| − | {{{tech}}}
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <p>3A assembly was conducted according to the <a href="http://parts.igem.org/Help:Protocol/3A_Assembly">iGEM 3A assembly protocol</a>.</p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <div class="nb-week" id="week12entry" style="display: block">
| |
| − | <div class="twelve columns">
| |
| − | <h3 class="centered">Ligation</h3>
| |
| − |
| |
| − |
| |
| − | <div class="entry pc-techniques">
| |
| − | <div>
| |
| − | <div class="nb-tech">
| |
| − | {{{tech}}}
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <p>Ligation was performed according to the protocol outlined on the <a href="http://unitedscientists.org/labs/norway/NTNU/PhotoSynLab/wiki/table-of-contents-2/protocol-molecular/pcr-touchdown-pcr">PhotoSynLab wiki</a>.</p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <div class="nb-week" id="week13entry" style="display: block">
| |
| − | <div class="twelve columns">
| |
| − | <h3 class="centered">Transformation (Escherichia coli)</h3>
| |
| − |
| |
| − |
| |
| − | <p>
| |
| − | </p>
| |
| − |
| |
| − |
| |
| − | <div class="entry pc-techniques">
| |
| − | <div>
| |
| − | <div class="nb-tech">
| |
| − | {{{tech}}}
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <p>The protocols used for preparing competent DH5a cells, as well as the heat-shock transformation procedure employed can be found at the <a href="http://unitedscientists.org/labs/norway/NTNU/PhotoSynLab/wiki/table-of-contents-2/protocol-molecular/heat-shock-transformation-2">PhotoSynLab wiki</a>.</p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <div class="nb-week" id="week14entry" style="display: block">
| |
| − | <div class="twelve columns">
| |
| − | <h3 class="centered">Transformation (Synechocystis sp. PCC 6803)</h3>
| |
| − |
| |
| − |
| |
| − | <p>
| |
| − | </p>
| |
| − |
| |
| − |
| |
| − | <div class="entry pc-techniques">
| |
| − | <div>
| |
| − | <div class="nb-tech">
| |
| − | {{{tech}}}
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <p>The transformation procedure for transforming Synechocystis can be found at the <a href="http://unitedscientists.org/labs/norway/NTNU/PhotoSynLab/wiki/table-of-contents-2/protocol-molecular/synechocystis-transformation-r-hill-method">PhotoSynLab wiki</a>.</p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <div class="nb-week" id="week16entry" style="display: block">
| |
| − | <div class="twelve columns">
| |
| − | <h3 class="centered">Right flank</h3>
| |
| − |
| |
| − |
| |
| − | <div class="entry pc-plas">
| |
| − | <div>
| |
| − | <div class="nb-tech">
| |
| − | {{{tech}}}
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <p><a href="http://parts.igem.org/Part:BBa_K1424001">BBa_K1424001</a>.</p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <div class="nb-week" id="week17entry" style="display: block">
| |
| − | <div class="twelve columns">
| |
| − | <h3 class="centered">Left flank</h3>
| |
| − |
| |
| − |
| |
| − | <div class="entry pc-plas">
| |
| − | <div>
| |
| − | <div class="nb-tech">
| |
| − | {{{tech}}}
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <p><a href="http://parts.igem.org/Part:BBa_K1424000">BBa_K1424000</a>.</p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <div class="nb-week" id="week18entry" style="display: block">
| |
| − | <div class="twelve columns">
| |
| − | <h3 class="centered">Kanamycin resistance</h3>
| |
| − |
| |
| − |
| |
| − | <div class="entry pc-plas">
| |
| − | <div>
| |
| − | <div class="nb-tech">
| |
| − | {{{tech}}}
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <p><a href="http://parts.igem.org/Part:BBa_K1424003">BBa_K1424003</a>.</p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <div class="nb-week" id="week19entry" style="display: block">
| |
| − | <div class="twelve columns">
| |
| − | <h3 class="centered">Lac promotor</h3>
| |
| − |
| |
| − |
| |
| − | <div class="entry pc-plas">
| |
| − | <div>
| |
| − | <div class="nb-tech">
| |
| − | {{{tech}}}
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <p><a href="http://parts.igem.org/Part:BBa_K1424002">BBa_K1424002</a>.</p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <div class="nb-week" id="week20entry" style="display: block">
| |
| − | <div class="twelve columns">
| |
| − | <h3 class="centered">Glucose oxidase</h3>
| |
| − |
| |
| − |
| |
| − | <div class="entry pc-plas">
| |
| − | <div>
| |
| − | <div class="nb-tech">
| |
| − | {{{tech}}}
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <p><a href="http://parts.igem.org/Part:BBa_K1424004">BBa_K1424004</a>.</p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <div class="nb-week" id="week21entry" style="display: block">
| |
| − | <div class="twelve columns">
| |
| − | <h3 class="centered">Left flank + Kanamycin resistance + Right flank</h3>
| |
| − |
| |
| − |
| |
| − | <div class="entry pc-plas">
| |
| − | <div>
| |
| − | <div class="nb-tech">
| |
| − | {{{tech}}}
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <p><a href="http://parts.igem.org/Part:BBa_K1424005">BBa_K1424005</a>.</p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <div class="nb-week" id="week24entry" style="display: block">
| |
| − | <div class="twelve columns">
| |
| − | <h3 class="centered">DNA concentration</h3>
| |
| − |
| |
| − |
| |
| − | <div class="entry pc-calc">
| |
| − | <div>
| |
| − | <div class="nb-tech">
| |
| − | {{{tech}}}
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <p>After measuring the concentration of a sample of DNA in ng/ul with the NanoDrop method, the concentration in terms of pmolar could be determined with the equation <img src="https://static.igem.org/mediawiki/2014/c/c5/Eqn3.gif"></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | </body>
| |
| − | </html>
| |