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| <img src="https://static.igem.org/mediawiki/2015/c/c3/Nankai_γPGAproduct.jpg"> | | <img src="https://static.igem.org/mediawiki/2015/c/c3/Nankai_γPGAproduct.jpg"> |
− | <p>What is “Pudding Health kit”? The Pudding Health kit is actually a jelly-like unguent made of Poly-γ-glutamic acid (γ-PGA) and medicine, which has a significantly better effect on healing the wounds than usual unguents. | + | <p>What is “Pudding Health kit”? The Pudding Health kit is actually a jelly-like unguent made of Poly-γ-glutamic acid (γ-PGA) and medicine, which has a significantly better effect on healing the wounds than usual unguents. </p> |
− | What is Poly-γ-glutamic acid? Poly-γ-glutamic acid, i.e. γ-PGA, is an important, naturally occurring polyamide which exhibits excellent peculiarity in water retention and sustained drug release. How is γ-PGA produced? We used engineered Bacillus amyloliquefaciens LL3 for mass production of γ-PGA, yet the original B. amyloliquefaciens LL3 strain that we isolated from fermented food could only produce γ-PGA at a very low yield. | + | <p>What is Poly-γ-glutamic acid? Poly-γ-glutamic acid, i.e. γ-PGA, is an important, naturally occurring polyamide which exhibits excellent peculiarity in water retention and sustained drug release. How is γ-PGA produced? We used engineered Bacillus amyloliquefaciens LL3 for mass production of γ-PGA, yet the original B. amyloliquefaciens LL3 strain that we isolated from fermented food could only produce γ-PGA at a very low yield.</p> |
− | Wonder how we did it?<a href="https://2015.igem.org/Team:Nankai/Description"> Click here to learn more………</a></p> | + | <p>Wonder how we did it?<a href="https://2015.igem.org/Team:Nankai/Description"> Click here to learn more………</a></p> |
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| + | <h4> We use modeling in our project.</h4> |
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| + | <img src="https://static.igem.org/mediawiki/2015/4/4b/Nankai_statistic.jpg"> |
| + | <p>The purpose of our project is to improve the output of polyglutamic acid. But how do we evaluate our work? That's why we need modeling, a useful tool to check our plans and enhance their feasibility.</p> |
| + | Considering our project is aimed at industrial production, we use our modeling to fit those macro variables which are quite useful in the fermentation industry. They are bacteria scale, product concentration and substrate concentration. Actually what we are most concerned with is the product, so its modeling is necessary. Then we need other equations to illustrate the product. Here we can also choose the gene express pathway using Michealis equation to build our modeling. However, as I said before, we want our data and methods can be used in the industrial fermentation. With this modeling, we can easily find out a suitable fermentation environment and calculate the benefits. |
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