Difference between revisions of "Team:Nankai"

 
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<p>Your place:&nbsp;<a href="https://2015.igem.org/Team:Nankai">Home</a></p>
 
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        <h1>Welcome to Nankai IGEM team!</h1>
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        <h4>From collage of life science</h4>
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        <h1>Welcome to Nankai IGEM Team!</h1>
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        <h4>From College of Life Sciences, Nankai University.</h4>
 
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<h3>Our fun project!</h3>
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<h3>This autumn, we're coming!</h3>
 
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<span class="meta-category">This is where we do our project.</span>
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<img src="https://static.igem.org/mediawiki/2015/9/93/Nankai_pic2.JPG" alt="This is where we do our project.">
 
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<img src="https://static.igem.org/mediawiki/2015/7/76/Nankai_pic3.JPG" alt="Our kind professor.">
 
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<span class="meta-category">We have a cute captain.</span>
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<h3 class="portfolio-title"><a href="https://static.igem.org/mediawiki/2015/d/da/Nankai_pic4.JPG" target="_blank">Original Picture</a></h3>
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<span class="meta-category">Conferences are just our routine.</span>
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<h3 class="portfolio-title"><a href="https://static.igem.org/mediawiki/2015/d/dc/Nankai_pic5.JPG" target="_blank">Original Picture</a></h3>
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<span class="meta-category">Everyone is working hard.</span>
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<h3 class="portfolio-title"><a href="https://static.igem.org/mediawiki/2015/b/b0/Nankai_pic6.JPG" target="_blank">Original Picture</a></h3>
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<span class="meta-category">We are the Nankai 2015 team .</br>We are the member of college of life sciences.</span>
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<h3 class="portfolio-title"><a href="https://static.igem.org/mediawiki/2015/2/2b/Nankai_pic7.JPG" target="_blank">Original Picture</a></h3>
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<img src="https://static.igem.org/mediawiki/2015/2/2b/Nankai_pic7.JPG" alt="We are the Nankai 2015 team .</br>We are the member of college of life sciences.">
 
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<h4> This is our team!</h4>
 
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<h4>Project Description</h4>
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<img src="https://static.igem.org/mediawiki/2015/8/80/Nankai_group_photo.JPG">
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<p>Team Nankai 2015 is consist of 9 junior students, 11 senior students and 5 instructors. With our team established in March 2015, we are more than excited to join the big community of iGEM.</p>
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Poly-γ-glutamic acid (γ-PGA) is an important,  naturally occurring polyamide consisting of D/L-glutamate monomers. Unlike  typical peptide linkages, the amide linkages inγ-PGA are  formed between the α-amino group and the  γ-carboxyl group. γ-PGA exhibits many favorable features such as biodegradable, water soluble, edible and non-toxic to humans and the environment. Therefore,  it has been widely used in fields of foods, medicines, cosmetics and agriculture and many unique applications, such as a sustained release material  and drug carrier, curable biological adhesive, biodegradable fibres, and highly  water absorbable hydrogels.<br>
+
<p>The idea of synthetic biology attracted us in a way that other fields of study never could, and our passion about science is the engine that has led us all the way through obstacles and setbacks. We are persevering and capable as a research team. However, at spare times, we are just a group of fun-loving young college students. There is always an atmosphere of anticipation that we could blend into iGEM community. So we are looking forward to seeing you at the Giant Jamboree! Want to know more about us? <a href="https://2015.igem.org/Team:Nankai/Team">click here……</a></p>
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  Strains capable for producing γ-PGA  are divided into two categories based on their requirement for glutamate acid:  glutamate-dependent strains and glutamate-independent strains. Glutamate-independent strains are preferable for industrial production because  of their low cost and simplified fermentation process. However, compared with  glutamate-dependent strains, their lower γ-PGA productivity limits their  industrial application.  Therefore,  the construction of a glutamate-independent strain with high γ-PGA yield is important for industrial applications. <br>
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  <em>Bacillusamyloliquefaciens</em> LL3, isolated from fermented food, is a  glutamate-independent strain, which can produce 3-4 g/L γ-PGA with sucrose as  its carbon source and ammonium sulfate as its nitrogen source. The <em>B. amyloliquefaciens</em> LL3 strain was deposited in the China Center for Type Culture  Collection (CCTCC) with accession number CCTCC M 208109 and its whole genome has been sequenced in 2011. In this study,  we aimed to improve the γ-PGA production based on the <em>B. amyloliquefaciens </em>NK-1 strain (a derivative of LL3 strain with  its endogenous plasmid and <em>upp</em> gene  deleted).<br>
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<h4> Do you know……?</h4>
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In order to improve  γ-PGA production, we employed two strategies to fine-tune the synthetic  pathways and balance the metabolism in the glutamate-independent <em>B. amyloliquefaciens </em>NK-1 strain. Firstly, we constructed a metabolic toggle switch in the NK-1 strain to inhibit the expression of ODHC (2-oxoglutarate dehydrogenase complex) by adding IPTG in the stationary stage and distribute the metabolic flux more frequently to be used  for γ-PGA precursor-glutamate synthesis. As scientists had found that the activity of ODHC was rather  low when glutamate was highly produced in a <em>Corynebacterium&nbsp;glutamicum</em> strain. Second, to balance the increase  of endogenous glutamate production, we optimized the expression level of <em>pgsBCA</em> genes (responsible for γ-PGA synthesis) by replacing its native promoter to seven different strength of promoters. Through these two strategies, we aimed  to obtain a γ-PGA production improved mutant strain. </p>
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<div class="text">
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<img src="https://static.igem.org/mediawiki/2015/c/c3/Nankai_γPGAproduct.jpg">
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<p>What is “Pudding Health kit”? Pudding Health kit is actually a jelly-like unguent made of poly-γ-glutamic acid (γ-PGA) and wound healing medicines. It aims at mitigating prolonged inflammations during the process of tissue regeneration, and speeding up the healing process of chronic wounds like diabetic wounds. </p>
 +
<p>What is Poly-γ-glutamic acid? Poly-γ-glutamic acid, i.e. γ-PGA, is an important, naturally occurring polyamide which exhibits excellent peculiarity in water retention and sustained drug release. How is γ-PGA produced? We used engineered <em>Bacillus amyloliquefaciens</em> LL3 for mass production of γ-PGA, yet the original <em>B. amyloliquefaciens</em> LL3 strain that we isolated from fermented food could only produce γ-PGA at a very low yield.</p>
 +
<p>Wonder how we did it?<a href="https://2015.igem.org/Team:Nankai/Description"> Click here to learn more………</a></p>
 +
</div>
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<div class="content rightfloat">
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<h4> We use modeling in our project.</h4>
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<div class="text">
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<img src="https://static.igem.org/mediawiki/2015/4/4b/Nankai_statistic.jpg">
 +
<p>Although our project is promising and exciting, there are many difficulties when it comes to the practical fermentation. We need to consider lots of things before we can get our precious health pudding kit. For example, what culture medium should we use? How much carbon source should we add? How long should the fermentation process be? Well, we can hardly get what we want if it’s too short. On the other hand, a too long process is a waste of source and time, and you know, time means money in the industrial production. Most importantly, will our operation be harmful to the bacteria so that they can’t even survive? These are all we should take care of. </p>
 +
<p>These practical questions are to be solved by our modeling. For details, <a href="https://2015.igem.org/Team:Nankai/Modeling">click here.</a>
 +
</p>
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<h4> Outside the lab……</h4>
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<div class="text">
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<img class="bottom" src="https://static.igem.org/mediawiki/2015/6/6d/Nankai_HP.JPG">
 +
<p>As Peter Carr puts it, "Human Practices is the study of how your work affects the world, and how the world affects your work." This year, our team communicated with lots of teams and exchange our ideas.We also bravely showed our ideads on national confenrence. We were in Beijing, Tianjin, Zhejiang Provicnce and so on. Through communications, our team are growing and our ideas are spreading! <a href="https://2015.igem.org/Team:Nankai/Practices">Here for more information.</a></p>
 +
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<section class="iShare">
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<div class="content">
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<h2>Our feature: iShare!</h2>
 +
<p>As we all know iGEM has already established a functional platform for participators to share Bio-bricks. However materials related to those Bio-bricks are still missing, like bacterial strains and expression vectors. It’s difficult to repeat previous iGEM projects and we want to make a difference by introducing iShare to all the iGEMers.</p>
 +
<p>iShare as we pictured is the first step to all-round resource sharing platform. We suggest iGEM teams put information of materials used in their project on iShare and label those they are willing to share. In this way, future teams could have more resources for similar work and may come up with more improvement.Please see more information on <a href="https://2015.igem.org/Team:Nankai/Practices/iShare">iShare</a>.</p>
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Latest revision as of 15:58, 18 September 2015

Medigo Blue, free responsive template

Your place: Home

Welcome to Nankai IGEM Team!

From College of Life Sciences, Nankai University.

This autumn, we're coming!

This is our team!

Team Nankai 2015 is consist of 9 junior students, 11 senior students and 5 instructors. With our team established in March 2015, we are more than excited to join the big community of iGEM.

The idea of synthetic biology attracted us in a way that other fields of study never could, and our passion about science is the engine that has led us all the way through obstacles and setbacks. We are persevering and capable as a research team. However, at spare times, we are just a group of fun-loving young college students. There is always an atmosphere of anticipation that we could blend into iGEM community. So we are looking forward to seeing you at the Giant Jamboree! Want to know more about us? click here……

Do you know……?

What is “Pudding Health kit”? Pudding Health kit is actually a jelly-like unguent made of poly-γ-glutamic acid (γ-PGA) and wound healing medicines. It aims at mitigating prolonged inflammations during the process of tissue regeneration, and speeding up the healing process of chronic wounds like diabetic wounds.

What is Poly-γ-glutamic acid? Poly-γ-glutamic acid, i.e. γ-PGA, is an important, naturally occurring polyamide which exhibits excellent peculiarity in water retention and sustained drug release. How is γ-PGA produced? We used engineered Bacillus amyloliquefaciens LL3 for mass production of γ-PGA, yet the original B. amyloliquefaciens LL3 strain that we isolated from fermented food could only produce γ-PGA at a very low yield.

Wonder how we did it? Click here to learn more………

We use modeling in our project.

Although our project is promising and exciting, there are many difficulties when it comes to the practical fermentation. We need to consider lots of things before we can get our precious health pudding kit. For example, what culture medium should we use? How much carbon source should we add? How long should the fermentation process be? Well, we can hardly get what we want if it’s too short. On the other hand, a too long process is a waste of source and time, and you know, time means money in the industrial production. Most importantly, will our operation be harmful to the bacteria so that they can’t even survive? These are all we should take care of.

These practical questions are to be solved by our modeling. For details, click here.

Outside the lab……

As Peter Carr puts it, "Human Practices is the study of how your work affects the world, and how the world affects your work." This year, our team communicated with lots of teams and exchange our ideas.We also bravely showed our ideads on national confenrence. We were in Beijing, Tianjin, Zhejiang Provicnce and so on. Through communications, our team are growing and our ideas are spreading! Here for more information.

Our feature: iShare!

As we all know iGEM has already established a functional platform for participators to share Bio-bricks. However materials related to those Bio-bricks are still missing, like bacterial strains and expression vectors. It’s difficult to repeat previous iGEM projects and we want to make a difference by introducing iShare to all the iGEMers.

iShare as we pictured is the first step to all-round resource sharing platform. We suggest iGEM teams put information of materials used in their project on iShare and label those they are willing to share. In this way, future teams could have more resources for similar work and may come up with more improvement.Please see more information on iShare.

Medigo Blue, free responsive template

Copyright © Nankai University

Nankai IGEM Team 2015