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− | <html lang="en-US"> | + | <html> |
− | <body>
| + | <head> |
− |
| + | <style type="text/css"> |
− | <section id="homeIntro" class="parallax first-widget">
| + | section { |
| + | margin-top:100px; |
| + | width:100%; |
| + | } |
| + | </style> |
| + | </head> |
| + | <section id="homeIntro" class="parallax first-widget"> |
| <div class="parallax-overlay"> | | <div class="parallax-overlay"> |
| <div class="container home-intro-content"> | | <div class="container home-intro-content"> |
| <div class="row"> | | <div class="row"> |
| <div class="col-md-12"> | | <div class="col-md-12"> |
− | <h2>Welcome to Nankai iGEM 2015!</h2> | + | <h2>Welcome to Nankai IGEM team!</h2> |
− | <p>This is a place where you can find the most inspiring ideas and the greatest enthusiasm in Synthetic Biology </p> | + | <p>From collage of life science</p> |
− | </div> <!-- /.col-md-12 -->
| + | <a href="#" class="large-button white-color">Enter <i class="icon-button fa fa-download"></i></a> |
− | </div> <!-- /.row -->
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− | </div> <!-- /.container -->
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− | </div> <!-- /.parallax-overlay -->
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− | </section> <!-- /#homeIntro -->
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− | <div class="container">
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− | <div class="row">
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− | <div class="col-md-4 col-sm-4">
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− | <div class="service-box-wrap">
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− | <div class="service-icon-wrap">
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− | <img src="https://static.igem.org/mediawiki/2015/a/a1/Nankai_sign.jpg" alt=""/>
| + | |
− | </div> <!-- /.service-icon-wrap -->
| + | |
− | <div class="service-cnt-wrap">
| + | |
− | <h3 class="service-title">Easy Theme Colors</h3>
| + | |
− | <p>There are 4 color themes (blue, green, red, orange) and this one is blue.</p>
| + | |
− | </div> <!-- /.service-cnt-wrap -->
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− | </div> <!-- /.service-box-wrap -->
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− | </div> <!-- /.col-md-4 -->
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− | <div class="col-md-4 col-sm-4">
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− | <div class="service-box-wrap">
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− | <div class="service-icon-wrap">
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− | <img src="https://static.igem.org/mediawiki/2015/a/a1/Nankai_sign.jpg" alt=""/> </div> <!-- /.service-icon-wrap -->
| + | |
− | <div class="service-cnt-wrap">
| + | |
− | <h3 class="service-title">Change Icons</h3>
| + | |
− | <p>Check <a rel="nofollow" href="http://fontawesome.io/icons/" target="_blank">FontAwesome</a> for your suitable icons. Example: <i class="fa fa-download"></i></p>
| + | |
− | </div> <!-- /.service-cnt-wrap -->
| + | |
− | </div> <!-- /.service-box-wrap -->
| + | |
− | </div> <!-- /.col-md-4 -->
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− | <div class="col-md-4 col-sm-4">
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− | <div class="service-box-wrap">
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− | <div class="service-icon-wrap">
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− | <img src="https://static.igem.org/mediawiki/2015/a/a1/Nankai_sign.jpg" alt=""/> </div> <!-- /.service-icon-wrap -->
| + | |
− | <div class="service-cnt-wrap">
| + | |
− | <h3 class="service-title">Pixel Perfect Design</h3>
| + | |
− | <p>Based on a 12 column grid system, with every pixel snapped.</p>
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− | </div> <!-- /.service-cnt-wrap -->
| + | |
− | </div> <!-- /.service-box-wrap -->
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− | </div> <!-- /.col-md-4 -->
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− | <div class="row">
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− | <div class="col-md-4 col-sm-4">
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− | <div class="service-box-wrap">
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− | <div class="service-icon-wrap">
| + | |
− | <img src="https://static.igem.org/mediawiki/2015/a/a1/Nankai_sign.jpg" alt=""/> </div> <!-- /.service-icon-wrap -->
| + | |
− | <div class="service-cnt-wrap">
| + | |
− | <h3 class="service-title">Valid HTML5</h3>
| + | |
− | <p>We offer validated html files and well commented code on our themes.</p>
| + | |
− | </div> <!-- /.service-cnt-wrap -->
| + | |
− | </div> <!-- /.service-box-wrap -->
| + | |
− | </div> <!-- /.col-md-4 -->
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− | <div class="col-md-4 col-sm-4">
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− | <div class="service-box-wrap">
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− | <div class="service-icon-wrap">
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− | <img src="https://static.igem.org/mediawiki/2015/a/a1/Nankai_sign.jpg" alt=""/> </div> <!-- /.service-icon-wrap -->
| + | |
− | <div class="service-cnt-wrap">
| + | |
− | <h3 class="service-title">Retina Ready</h3>
| + | |
− | <p>Incredibly clean design provides you powerful way to grow your business.</p>
| + | |
− | </div> <!-- /.service-cnt-wrap -->
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− | </div> <!-- /.service-box-wrap -->
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− | </div> <!-- /.col-md-4 -->
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− | <div class="col-md-4 col-sm-4">
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− | <div class="service-box-wrap">
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− | <div class="service-icon-wrap">
| + | |
− | <img src="https://static.igem.org/mediawiki/2015/a/a1/Nankai_sign.jpg" alt=""/> </div> <!-- /.service-icon-wrap -->
| + | |
− | <div class="service-cnt-wrap">
| + | |
− | <h3 class="service-title">Special Thanks</h3>
| + | |
− | <p>Credit goes to <a rel="nofollow" href="http://unsplash.com" target="_blank">Unsplash</a> for images used in this template.</p>
| + | |
− | </div> <!-- /.service-cnt-wrap -->
| + | |
− | </div> <!-- /.service-box-wrap -->
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− | </div> <!-- /.col-md-4 -->
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− | </div> <!-- /.row -->
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− | </div> <!-- /.container -->
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− | </section> <!-- /.services -->
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− | <section class="dark-content portfolio">
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− | <div class="container">
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− | <div class="row">
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− | <div class="col-md-12">
| + | |
− | <div class="section-header">
| + | |
− | <h2 class="section-title">Our Lovely Portfolio</h2>
| + | |
− | <p class="section-desc">Everything you need to create a professional website.</p>
| + | |
− | </div> <!-- /.section-header -->
| + | |
− | </div> <!-- /.col-md-12 -->
| + | |
− | </div> <!-- /.row -->
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− | </div> <!-- /.container -->
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− |
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− | <div id="portfolio-carousel" class="portfolio-carousel portfolio-holder">
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− | <div class="item">
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− | <div class="thumb-post">
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− | <div class="overlay">
| + | |
− | <div class="overlay-inner">
| + | |
− | <div class="portfolio-infos">
| + | |
− | <span class="meta-category">Dashboard Template</span>
| + | |
− | <h3 class="portfolio-title"><a href="project-slideshow.html">Visual Admin</a></h3>
| + | |
− | </div>
| + | |
− | <div class="portfolio-expand">
| + | |
− | <a class="fancybox" href="images/includes/project1.jpg" title="Visual Admin">
| + | |
− | <i class="fa fa-expand"></i>
| + | |
− | </a>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <img src="images/includes/project1.jpg" alt="Visual Admin">
| + | |
− | </div>
| + | |
− | </div> <!-- /.item -->
| + | |
− | <div class="item">
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− | <div class="thumb-post">
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− | <div class="overlay">
| + | |
− | <div class="overlay-inner">
| + | |
− | <div class="portfolio-infos">
| + | |
− | <span class="meta-category">Mobile Ready</span>
| + | |
− | <h3 class="portfolio-title"><a href="project-slideshow.html">Compass Template</a></h3>
| + | |
− | </div>
| + | |
− | <div class="portfolio-expand">
| + | |
− | <a class="fancybox" href="images/includes/project2.jpg" title="Compass Template">
| + | |
− | <i class="fa fa-expand"></i>
| + | |
− | </a>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <img src="images/includes/project2.jpg" alt="Compass Template">
| + | |
− | </div>
| + | |
− | </div> <!-- /.item -->
| + | |
− | <div class="item">
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− | <div class="thumb-post">
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− | <div class="overlay">
| + | |
− | <div class="overlay-inner">
| + | |
− | <div class="portfolio-infos">
| + | |
− | <span class="meta-category">Responsive Design</span>
| + | |
− | <h3 class="portfolio-title"><a href="project-slideshow.html">Awesome Theme</a></h3>
| + | |
− | </div>
| + | |
− | <div class="portfolio-expand">
| + | |
− | <a class="fancybox" href="images/includes/project3.jpg" title="Awesome Theme">
| + | |
− | <i class="fa fa-expand"></i>
| + | |
− | </a>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <img src="images/includes/project3.jpg" alt="Awesome Theme">
| + | |
− | </div>
| + | |
− | </div> <!-- /.item -->
| + | |
− | <div class="item">
| + | |
− | <div class="thumb-post">
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− | <div class="overlay">
| + | |
− | <div class="overlay-inner">
| + | |
− | <div class="portfolio-infos">
| + | |
− | <span class="meta-category">Portfolio</span>
| + | |
− | <h3 class="portfolio-title"><a href="project-slideshow.html">Volton Personal Site</a></h3>
| + | |
− | </div>
| + | |
− | <div class="portfolio-expand">
| + | |
− | <a class="fancybox" href="images/includes/project4.jpg" title="Volton Personal Site">
| + | |
− | <i class="fa fa-expand"></i>
| + | |
− | </a>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <img src="images/includes/project4.jpg" alt="Volton Personal Site">
| + | |
− | </div>
| + | |
− | </div> <!-- /.item -->
| + | |
− | <div class="item">
| + | |
− | <div class="thumb-post">
| + | |
− | <div class="overlay">
| + | |
− | <div class="overlay-inner">
| + | |
− | <div class="portfolio-infos">
| + | |
− | <span class="meta-category">Green</span>
| + | |
− | <h3 class="portfolio-title"><a href="project-slideshow.html">Rectangle Design</a></h3>
| + | |
− | </div>
| + | |
− | <div class="portfolio-expand">
| + | |
− | <a class="fancybox" href="images/includes/project5.jpg" title="Rectangle Design">
| + | |
− | <i class="fa fa-expand"></i>
| + | |
− | </a>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <img src="images/includes/project5.jpg" alt="Rectangle Design">
| + | |
− | </div>
| + | |
− | </div> <!-- /.item -->
| + | |
− | <div class="item">
| + | |
− | <div class="thumb-post">
| + | |
− | <div class="overlay">
| + | |
− | <div class="overlay-inner">
| + | |
− | <div class="portfolio-infos">
| + | |
− | <span class="meta-category">Portfolio</span>
| + | |
− | <h3 class="portfolio-title"><a href="project-slideshow.html">Masonry Gallery</a></h3>
| + | |
− | </div>
| + | |
− | <div class="portfolio-expand">
| + | |
− | <a class="fancybox" href="images/includes/project6.jpg" title="Masonry Gallery">
| + | |
− | <i class="fa fa-expand"></i>
| + | |
− | </a>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <img src="images/includes/project6.jpg" alt="Masonry Gallery">
| + | |
− | </div>
| + | |
− | </div> <!-- /.item -->
| + | |
− | <div class="item">
| + | |
− | <div class="thumb-post">
| + | |
− | <div class="overlay">
| + | |
− | <div class="overlay-inner">
| + | |
− | <div class="portfolio-infos">
| + | |
− | <span class="meta-category">Branding / Identity</span>
| + | |
− | <h3 class="portfolio-title"><a href="project-slideshow.html">Gloss Template</a></h3>
| + | |
− | </div>
| + | |
− | <div class="portfolio-expand">
| + | |
− | <a class="fancybox" href="images/includes/project7.jpg" title="Gloss Template">
| + | |
− | <i class="fa fa-expand"></i>
| + | |
− | </a>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <img src="images/includes/project7.jpg" alt="Gloss Template">
| + | |
− | </div>
| + | |
− | </div> <!-- /.item -->
| + | |
− | </div> <!-- /#owl-demo -->
| + | |
− | </section> <!-- /.portfolio-holder -->
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− | | + | |
− | <section class="testimonials-widget">
| + | |
− | <div class="container">
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− | <div class="row">
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− | <div class="col-md-12">
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− | <div class="bxslider">
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− | <div class="testimonial">
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− | <div class="testimonial-content">
| + | |
− | <span class="testimonial-author">Andy Colin - Architect</span>
| + | |
− | <p class="testimonial-description">Thank you for all your good work in creating theme. They have a ‘presence’ which feels right.</p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <div class="testimonial">
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− | <div class="testimonial-content">
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− | <span class="testimonial-author">Thomas Teddy - Design Writer</span>
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− | <p class="testimonial-description">I love the logo. Particularly how the mark can stand on its own. Nice work! It feels tall and proud and powerful — and I love that. That’s what I was after.</p>
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− | </div>
| + | |
− | </div>
| + | |
− | <div class="testimonial">
| + | |
− | <div class="testimonial-content">
| + | |
− | <span class="testimonial-author">John Willy - Developer</span>
| + | |
− | <p class="testimonial-description">You’re pretty awesome to work with. Incredibly organized, easy to communicate with, responsive with next iterations, and beautiful work.</p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div> <!-- /.bxslider -->
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− | </div> <!-- /.col-md-12 -->
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− | </div> <!-- /.row -->
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− | </div> <!-- /.container -->
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− | </section> <!-- /.testimonials-widget -->
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− | | + | |
− | <section class="light-content our-team">
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− | <div class="container">
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− | <div class="row">
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− | <div class="col-md-12">
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− | <div class="section-header">
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− | <h2 class="section-title">Meet our happy staff</h2>
| + | |
− | <p class="section-desc">Medigo is the automated way to keep track of what everyone is working on.</p>
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− | </div> <!-- /.section-header -->
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− | </div> <!-- /.col-md-12 -->
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− | </div> <!-- /.row -->
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− | </div> <!-- /.container -->
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− | <div class="team-members">
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− | <div class="container">
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− | <div class="row">
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− | <div class="col-md-4 col-sm-4">
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− | <div class="team-member">
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− | <div class="thumb-post">
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− | <img src="images/includes/member1.jpg" alt="">
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− | <span class="member-role">Marketing Manager</span>
| + | |
− | </div>
| + | |
− | <div class="member-content">
| + | |
− | <h4 class="member-name">Amy Groovy</h4>
| + | |
− | <p>Duis vitae consequat neque. Nulla pharetra eleifend nulla. </p>
| + | |
− | </div>
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− | </div> <!-- /.team-member -->
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− | </div> <!-- /.col-md-4 -->
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− | <div class="col-md-4 col-sm-4">
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− | <div class="team-member">
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− | <div class="thumb-post">
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− | <img src="images/includes/member2.jpg" alt="">
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− | <span class="member-role">Web Developer</span>
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− | </div>
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− | <div class="member-content">
| + | |
− | <h4 class="member-name">Candy Sharp</h4>
| + | |
− | <p>Duis vitae consequat neque. Nulla pharetra eleifend nulla. </p>
| + | |
− | </div>
| + | |
− | </div> <!-- /.team-member -->
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− | </div> <!-- /.col-md-4 -->
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− | | + | |
− | <div class="col-md-4 col-sm-4">
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− | <div class="team-member">
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− | <div class="thumb-post">
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− | <img src="images/includes/member3.jpg" alt="">
| + | |
− | <span class="member-role">Graphic Designer</span>
| + | |
− | </div>
| + | |
− | <div class="member-content">
| + | |
− | <h4 class="member-name">Linda Master</h4>
| + | |
− | <p>Duis vitae consequat neque. Nulla pharetra eleifend nulla. </p>
| + | |
− | </div>
| + | |
− | </div> <!-- /.team-member -->
| + | |
− | </div> <!-- /.col-md-4 -->
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− | | + | |
− | </div> <!-- /.row -->
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− | </div> <!-- /.container -->
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− | </div> <!-- /.team-members -->
| + | |
− | </section> <!-- /.our-team -->
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− |
| + | |
− | <section id="blogPosts" class="parallax">
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− | <div class="parallax-overlay">
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− | <div class="container">
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− | <div class="row">
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− | <div class="col-md-12">
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− | <div class="section-header">
| + | |
− | <h2 class="section-title">Recent from our blog</h2>
| + | |
− | <p class="section-desc">Everything you need to create a professional website.</p>
| + | |
− | </div> <!-- /.section-header -->
| + | |
| </div> <!-- /.col-md-12 --> | | </div> <!-- /.col-md-12 --> |
− | </div> <!-- /.row -->
| |
− | <div class="row latest-posts">
| |
− | <div class="col-md-4 col-sm-6">
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− | <div class="blog-post clearfix">
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− | <div class="thumb-post">
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− | <a href="blog-single.html"><img src="images/includes/blogthumb1.jpg" alt="" class="img-circle"></a>
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− | </div>
| |
− | <div class="blog-post-content">
| |
− | <h4 class="post-title"><a href="blog-single.html">Getting Creative With the Google Maps API</a></h4>
| |
− | <span class="meta-post-date">12 February 2084</span>
| |
− | </div>
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− | </div> <!-- /.blog-post -->
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− | </div> <!-- /.col-md-4 -->
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− | <div class="col-md-4 col-sm-6">
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− | <div class="blog-post clearfix">
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− | <div class="thumb-post">
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− | <a href="blog-single.html"><img src="images/includes/blogthumb2.jpg" alt="" class="img-circle"></a>
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− | </div>
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− | <div class="blog-post-content">
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− | <h4 class="post-title"><a href="blog-single.html">Design Deliverables – easily share project</a></h4>
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− | <span class="meta-post-date">10 February 2084</span>
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− | </div>
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− | </div> <!-- /.blog-post -->
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− | </div> <!-- /.col-md-4 -->
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− | <div class="col-md-4 col-sm-6">
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− | <div class="blog-post clearfix">
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− | <div class="thumb-post">
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− | <a href="blog-single.html"><img src="images/includes/blogthumb3.jpg" alt="" class="img-circle"></a>
| |
− | </div>
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− | <div class="blog-post-content">
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− | <h4 class="post-title"><a href="blog-single.html">Using Templates to Get Your Clients Thinking</a></h4>
| |
− | <span class="meta-post-date">8 February 2084</span>
| |
− | </div>
| |
− | </div> <!-- /.blog-post -->
| |
− | </div> <!-- /.col-md-4 -->
| |
| </div> <!-- /.row --> | | </div> <!-- /.row --> |
| </div> <!-- /.container --> | | </div> <!-- /.container --> |
| </div> <!-- /.parallax-overlay --> | | </div> <!-- /.parallax-overlay --> |
− | </section> <!-- /#blogPosts --> | + | </section> |
− | | + | <section> |
− |
| + | <h4>Project Description</h4> |
− | | + | <p> |
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| + | Poly-γ-glutamic acid (γ-PGA) is an important, naturally occurring polyamide consisting of D/L-glutamate monomers. Unlike typical peptide linkages, the amide linkages inγ-PGA are formed between the α-amino group and the γ-carboxyl group. γ-PGA exhibits many favorable features such as biodegradable, water soluble, edible and non-toxic to humans and the environment. Therefore, it has been widely used in fields of foods, medicines, cosmetics and agriculture and many unique applications, such as a sustained release material and drug carrier, curable biological adhesive, biodegradable fibres, and highly water absorbable hydrogels.<br> |
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| + | Strains capable for producing γ-PGA are divided into two categories based on their requirement for glutamate acid: glutamate-dependent strains and glutamate-independent strains. Glutamate-independent strains are preferable for industrial production because of their low cost and simplified fermentation process. However, compared with glutamate-dependent strains, their lower γ-PGA productivity limits their industrial application. Therefore, the construction of a glutamate-independent strain with high γ-PGA yield is important for industrial applications. <br> |
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− | </body> | + | <em>Bacillusamyloliquefaciens</em> LL3, isolated from fermented food, is a glutamate-independent strain, which can produce 3-4 g/L γ-PGA with sucrose as its carbon source and ammonium sulfate as its nitrogen source. The <em>B. amyloliquefaciens</em> LL3 strain was deposited in the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 208109 and its whole genome has been sequenced in 2011. In this study, we aimed to improve the γ-PGA production based on the <em>B. amyloliquefaciens </em>NK-1 strain (a derivative of LL3 strain with its endogenous plasmid and <em>upp</em> gene deleted).<br> |
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| + | In order to improve γ-PGA production, we employed two strategies to fine-tune the synthetic pathways and balance the metabolism in the glutamate-independent <em>B. amyloliquefaciens </em>NK-1 strain. Firstly, we constructed a metabolic toggle switch in the NK-1 strain to inhibit the expression of ODHC (2-oxoglutarate dehydrogenase complex) by adding IPTG in the stationary stage and distribute the metabolic flux more frequently to be used for γ-PGA precursor-glutamate synthesis. As scientists had found that the activity of ODHC was rather low when glutamate was highly produced in a <em>Corynebacterium glutamicum</em> strain. Second, to balance the increase of endogenous glutamate production, we optimized the expression level of <em>pgsBCA</em> genes (responsible for γ-PGA synthesis) by replacing its native promoter to seven different strength of promoters. Through these two strategies, we aimed to obtain a γ-PGA production improved mutant strain. </p> |
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