Team:Nankai/Collaborations
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Collaboration
As the famous Chinese proverb goes,"Union is strength". It adequately shows the importance of collaboration. During the past years, team Nankai has been cooperating intimately with team Tianjin, our beloved neighbor and friend. We discussed from time to time about different details in our projects and helped each other out when it was needed. Besides, this year team Nankai set up a new resource sharing platform named"iShare". It's a platform for all the iGEM teams to get what they need. To gather more the information, we have also reach for many other teams for help. Thanks to all the iGEM teams who offered us help, ishare is now fully functional.
Collaboration
I. Collaboration with our neighbor TJU
The Lotus Festival held at TJU
In early April, when the lotus is the most exuberant in school, team Tianjin held a lotus festival to popularize the knowledge of biotechnology. As their neighbor and partner,team Nankai were invited to the festival and we offered them several samples of actinomycetes for exhibition. During the festival we had some nice chats with members of team Tianjin and had a good time enjoying the beautiful view inside the campus of Tianjin University.
The front gate of TJU.
Poster for lotus festival.
Group photo at TJU.
Supporting date of hydrophobin expression
This year, iGEM team Tianjin worked on a special small protein called hydrophobin. They tried to optimize the expression of hydrophobin in prokaryotic expression system. However, a special member in hydrophobin group called injanus, also known as HGF1, could not be expressed in E.coli. They still needed the dates about the expression of injanus and thus reached us for help. Fortunately we managed to characterize the expression of injanus in Pichia pastoris,with the help of Qiao's laboratory in Nankai university. The results were sent to team Tianjin for further employment.
We employed fed-batch fermentation to produce injanus (HGFI), and injanus produced was detected in fermentation broth through HPLC (showed in Figure 1 and Figure 2). The spectra peak of pure injauns appeared around 30-32 mins, which showed the spectra peak appeared at around 33 mins in HPLC profiles of fermentation broth was injauns.
Figure 1. HPLC profiles of pure injanus.
Figure 2. HPLC profiles of fermentation broth.
Figure 3. Silver staining along with SDS-PAGE (a) and western blot analysis of fermentation broth (b). Injanus fermentation broth (Lane 2,3), pure injanus (Lane 1).
On the left are the real HPLC（High Performance Liquid Chromatography) results. From these pictures we can see that in the pure products the peak appeared at around 30 minutes while at the fermentation broth the peak appeared at aound 33 minutes.
Ⅱ. Collaboration with other teams for iShare
IShare is a brand new idea raised up by Nankai Team in 2014.It is an information-sharing platform aiming to converge all information of iGEM China teams involved in our experiment such as Topic, Introduction, Materials and Methods, Progression, Parts.In a word, it will be a platform which is beneficial for all the igem teams.
Therefore, during the summit of domestic igem teams held by Beijing University, we seized the opportunity to publicize our iShare platform by playing a short video and distributing questionnaires about it. Our goal was to gain their support and information as much as possible. Excitedly, this platform was supported by most present teams and we exactly got much information and advice, which give us much confidence and encouragement for building this platform!