Difference between revisions of "Team:Nankai/Parts"

 
(22 intermediate revisions by 5 users not shown)
Line 4: Line 4:
 
         <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Nankai/bootstrapCSS?action=raw&ctype=text/css" />
 
         <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Nankai/bootstrapCSS?action=raw&ctype=text/css" />
 
         <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Nankai/blue-schemeCSS?action=raw&ctype=text/css" />
 
         <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Nankai/blue-schemeCSS?action=raw&ctype=text/css" />
−
         <script src="https://2015.igem.org/Template:Nankai/jquery-1.10.2.minJS?action=raw&ctype=text/javascript"></script>
+
         <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Nankai/newCSS?action=raw&ctype=text/css" />
 
<style>
 
<style>
−
#globalWrapper,section{
 
−
min-width:1000px;
 
−
}
 
−
.container {
 
−
margin-left:5%;
 
−
margin-right:5%;
 
−
}
 
−
.sidebar {
 
−
margin-left:20%;
 
−
}
 
−
.sidebar p{
 
−
font-size:15px;
 
−
}
 
−
.sidebar-widget img{
 
−
width:100%;
 
−
margin-top:50px;
 
−
}
 
−
.container .row .col-md-8.blog-posts p {
 
−
text-indent: 40px;
 
−
}
 
−
.container .row .col-md-8.blog-posts h4 {
 
−
margin-top: 70px;
 
−
}
 
−
.sidebar-widget h6{
 
−
text-decoration: underline;
 
−
font-size:20px;
 
−
}
 
−
.sidebar-widget p{
 
−
text-align:center;
 
−
}
 
−
  /*Clear existing style*/
 
−
 
−
 
−
  #contentSub, #menubar, #footer-box, #siteSub, #catlinks,
 
−
 
−
#search-controls, #p-logo, .printfooter, .firstHeading,
 
−
 
−
.visualClear {
 
−
    display:none;
 
−
  }
 
−
 
−
  html, body {
 
−
    width: auto !important;
 
−
    /*overflow-x: hidden !important;*/
 
−
    height: 100% !important;
 
−
 
−
  }
 
−
  /*End claer*/
 
 
#blog {
 
#blog {
−
    
+
   background-image: url("https://static.igem.org/mediawiki/2015/6/62/Nankai_partpic.jpg");
−
  margin-top: 100px;
+
 
}
 
}
−
.col-md-8, .col-md-8:before, .col-md-8:after { -webkit-box-sizing: border-box; -moz-box-sizing: border-box; box-sizing: border-box; }
 
−
 
−
 
−
.blog-posts, .blog-posts:before, .blog-posts:after { -webkit-box-sizing: border-box; -moz-box-sizing: border-box; box-sizing: border-box; }
 
−
.col-md-12, .col-md-12:before, .col-md-12:after { -webkit-box-sizing: border-box; -moz-box-sizing: border-box; box-sizing: border-box; }
 
−
.blog-post, .blog-post:before, .blog-post:after { -webkit-box-sizing: border-box; -moz-box-sizing: border-box; box-sizing: border-box; }
 
−
.col-md-6, .col-md-6:before, .col-md-6:after { -webkit-box-sizing: border-box; -moz-box-sizing: border-box; box-sizing: border-box; }
 
−
.col-md-4, .col-md-4:before, .col-md-4:after { -webkit-box-sizing: border-box; -moz-box-sizing: border-box; box-sizing: border-box; }
 
 
</style>
 
</style>
  
Line 89: Line 32:
  
 
<div class="col-md-8 blog-posts">
 
<div class="col-md-8 blog-posts">
−
<h4>What is γ-PGA?</h4>
+
<h4>Parts Abstract</h4>
−
<p>Poly-γ-glutamic acid (γ-PGA) is an important, naturally occurring polyamide consisting of D/L-glutamate monomers. Unlike typical peptide linkages, the amide linkages in γ-PGA are formed between the α-amino group and the γ-carboxyl group. γ-PGA exhibits many favorable features such as biodegradable, water soluble, edible and non-toxic to humans and the environment. Therefore, it has been widely used in fields of foods, medicines, cosmetics and agriculture and many unique applications, such as a sustained release material and drug carrier, curable biological adhesive, biodegradable fibres, and highly water absorbable hydrogels.</p>
+
<table width="544" border="1":font-family:"Gill Sans MT",Consolas,Constantia;>
−
<h4>How can we produce it?</h4>
+
  <tr>
−
<p>Strains capable for producing γ-PGA are divided into two categories based on their requirement for glutamate acid: glutamate-dependent strains and glutamate-independent strains. Glutamate-independent strains are preferable for industrial production because of their low cost and simplified fermentation process. However, compared with glutamate-dependent strains, their lower γ-PGA productivity limits their industrial application.Therefore, the construction of a glutamate-independent strain with high γ-PGA yield is important for industrial applications.</p>
+
    <th width="78" bgcolor="#999999" scope="col">Number</th>
−
<h4>Who can produce it?</h4>
+
    <th width="45" bgcolor="#999999" scope="col">Name</th>
−
<p>Bacillusamyloliquefaciens LL3, isolated from fermented food, is a glutamate-independent strain, which can produce 3-4 g/L γ-PGA with sucrose as its carbon source and ammonium sulfate as its nitrogen source. The B. amyloliquefaciens LL3 strain was deposited in the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 208109 and its whole genome has been sequenced in 2011. In this study, we aimed to improve the γ-PGA production based on the B. amyloliquefaciens NK-1 strain (a derivative of LL3 strain with its endogenous plasmid and upp gene deleted).</p>
+
    <th width="77" bgcolor="#999999" scope="col">Type</th>
−
<h4>What did we do?</h4>
+
    <th width="178" bgcolor="#999999" scope="col">Description</th>
−
<p>In order to improve γ-PGA production, we employed two strategies to fine-tune the synthetic pathways and balance the metabolism in the glutamate-independent B. amyloliquefaciens NK-1 strain. Firstly, we constructed a metabolic toggle switch in the NK-1 strain to inhibit the expression of ODHC (2-oxoglutarate dehydrogenase complex) by adding IPTG in the stationary stage and distribute the metabolic flux more frequently to be used for γ-PGA precursor-glutamate synthesis. As scientists had found that the activity of ODHC was rather low when glutamate was highly produced in a Corynebacterium glutamicum strain. Second, to balance the increase of endogenous glutamate production, we optimized the expression level of pgsBCA genes (responsible for γ-PGA synthesis) by replacing its native promoter to seven different strength of promoters. Through these two strategies, we aimed to obtain a γ-PGA production improved mutant strain.<a href="https://2015.igem.org/Team:Nankai/Experiments">Click for more detail.</a></p>
+
    <th width="71" bgcolor="#999999" scope="col">Creator</th>
−
+
    <th width="55" bgcolor="#999999" scope="col">Length</th>
 +
  </tr>
 +
  <tr>
 +
    <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628001">BBa_K1628001</a></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">P<sub>bca</sub></span></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">Promoter</span></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq"> an original promoter of coding sequence <em>pgsBCA</em> operon</span></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">Tianyi Huang</span></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">364</span></td>
 +
  </tr>
 +
  <tr>
 +
    <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002">BBa_K1628002</a></td>
 +
    <td bgcolor="#FFFFFF"><span class="zaq">P<sub>xyl</sub></span></td>
 +
    <td bgcolor="#FFFFFF"><span class="zaq">Promoter</span></td>
 +
    <td bgcolor="#FFFFFF">a promoter of xylose operon</td>
 +
    <td bgcolor="#FFFFFF"><span class="zaq">Tianyi Huang</span></td>
 +
    <td bgcolor="#FFFFFF"><span class="zaq">217</span></td>
 +
  </tr>
 +
  <tr>
 +
    <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628003">BBa_K1628003</a></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">BJ27UP</span></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">Promoter</span></td>
 +
    <td bgcolor="#CCCCCC">an artificially synthesized promoter</td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">Xinhao Song</span></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">100</span></td>
 +
  </tr>
 +
  <tr>
 +
    <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628004">BBa_K1628004</a></td>
 +
    <td bgcolor="#FFFFFF"><span class="zaq">C2up</span></td>
 +
    <td bgcolor="#FFFFFF"><span class="zaq">Promoter</span></td>
 +
    <td bgcolor="#FFFFFF">an artificially synthesized promoter</td>
 +
    <td bgcolor="#FFFFFF"><span class="zaq">Xinhao Song</span></td>
 +
    <td bgcolor="#FFFFFF"><span class="zaq">108</span></td>
 +
  </tr>
 +
  <tr>
 +
    <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628005">BBa_K1628005</a></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">A2up</span></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">Promoter</span></td>
 +
    <td bgcolor="#CCCCCC">an artificially synthesized  promoter</td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">Yibing Wei</span></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">107</span></td>
 +
  </tr>
 +
  <tr bgcolor="#FFFFFF">
 +
    <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628006">BBa_K1628006</a></td>
 +
    <td><span class="zaq">P43</span></td>
 +
    <td><span class="zaq">Promoter</span></td>
 +
    <td>a strong promoter in <em>Bacillus subtilis </em>168</td>
 +
    <td><span class="zaq">Yibing Wei</span></td>
 +
    <td><span class="zaq">445</span></td>
 +
  </tr>
 +
  <tr>
 +
    <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628007">BBa_K1628007</a></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">P<sub>amyA</sub></span></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">Promoter</span></td>
 +
    <td bgcolor="#CCCCCC">a strong promoter in <em>Bacillus amyloliquefaciens </em>LL3</td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">Zhaoran Zhang</span></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">613</span></td>
 +
  </tr>
 +
  <tr bgcolor="#FFFFFF">
 +
    <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628101">BBa_K1628101</a></td>
 +
    <td><span class="zaq"><em>pgsB</em></span></td>
 +
    <td><span class="zaq">Coding</span></td>
 +
    <td>a gene responsible for  γ-PGA synthesis in <em>pgsBCA</em> operon</td>
 +
    <td><span class="zaq">Tianyi Huang</span></td>
 +
    <td><span class="zaq">1182</span></td>
 +
  </tr>
 +
  <tr>
 +
    <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628102">BBa_K1628102</a></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq"><em>pgsCA</em></span></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">Coding</span></td>
 +
    <td bgcolor="#CCCCCC">a coding gene in <em>pgsBCA</em> operon</td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">Xinhao Song</span></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">1631</span></td>
 +
  </tr>
 +
  <tr bgcolor="#FFFFFF">
 +
    <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628201">BBa_K1628201</a></td>
 +
    <td><span class="zaq">P<sub>lacI</sub>/lacI</span></td>
 +
    <td><span class="zaq">Translational Unit </span></td>
 +
    <td>a promoter of lactose operon along with a repressor of lactose operon regulating the promoter</td>
 +
    <td><span class="zaq">Yibing Wei</span></td>
 +
    <td><span class="zaq">1386</span></td>
 +
  </tr>
 +
  <tr>
 +
    <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628202">BBa_K1628202</a></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">P<sub>grac</sub></span></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">Promoter </span></td>
 +
    <td bgcolor="#CCCCCC">a promoter of lactose operon regulated by repressor LacI</td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">Tianyi Huang</span></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">120</span></td>
 +
  </tr>
 +
  <tr bgcolor="#FFFFFF">
 +
    <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628203">BBa_K1628203</a></td>
 +
    <td><span class="zaq">xylR</span></td>
 +
    <td><span class="zaq">Coding </span></td>
 +
    <td>a repressor of xylose operon regulating promoter <span class="zaq">P<sub>xyl</sub></span></td>
 +
    <td><span class="zaq">Zhaoran Zhang</span></td>
 +
    <td><span class="zaq">1167</span></td>
 +
  </tr>
 +
  <tr>
 +
    <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628301">BBa_K1628301</a></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">P1-GFP</span></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">Device</span></td>
 +
    <td bgcolor="#CCCCCC">P3-GFP is a composite part used for the Measurement track of this year. This part consists of the promoter part of BBa_J23117, the official GFP device (with an RBS, GFP coding sequence and a set of double terminators) and a pSB1C3 backbone. We transformed this plasmid into E. coli cells and the fluorescence intensity of the GFP protein that was expressed by the bacteria was measured afterwards by a flow cytometry.</td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">Tianyi Huang</span></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">?</span></td>
 +
  </tr>
 +
  <tr bgcolor="#FFFFFF">
 +
    <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628302">BBa_K1628202</a></td>
 +
    <td><span class="zaq">P2-GFP</span></td>
 +
    <td><span class="zaq">Device</span></td>
 +
    <td>P2-GFP is a composite part used for the Measurement track of this year. This part consists of the promoter part of BBa_J23106, the official GFP device (with an RBS, GFP coding sequence and a set of double terminators) and a pSB1C3 backbone. We transformed this plasmid into <em>E. coli</em> cells and the fluorescence intensity of the GFP protein that was expressed by the bacteria was measured afterwards by a flow cytometry.</td>
 +
    <td><span class="zaq">Xinhao Song</span></td>
 +
    <td><span class="zaq">?</span></td>
 +
  </tr>
 +
  <tr>
 +
    <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628303">BBa_K1628303</a></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">P3-GFP </span></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">Device</span></td>
 +
    <td bgcolor="#CCCCCC">P3-GFP is a composite part used for the Measurement track of this year. This part consists of the promoter part of BBa_J23117, the official GFP device (with an RBS, GFP coding sequence and a set of double terminators) and a pSB1C3 backbone. We transformed this plasmid into <em>E. coli </em>cells and the fluorescence intensity of the GFP protein that was expressed by the bacteria was measured afterwards by a flow cytometry.</td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">Yibing Wei</span></td>
 +
    <td bgcolor="#CCCCCC"><span class="zaq">?</span></td>
 +
  </tr>
 +
</table>
 +
 
</div> <!-- /.col-md-8 -->
 
</div> <!-- /.col-md-8 -->
  
Line 106: Line 172:
 
<h6><a href="https://2015.igem.org/Team:Nankai/Basic_Part">Basic Parts</a></h6>
 
<h6><a href="https://2015.igem.org/Team:Nankai/Basic_Part">Basic Parts</a></h6>
 
<h6><a href="https://2015.igem.org/Team:Nankai/Composite_Part">Composite Parts</a></h6>
 
<h6><a href="https://2015.igem.org/Team:Nankai/Composite_Part">Composite Parts</a></h6>
−
<h6><a href="https://2015.igem.org/Team:Nankai/Part_Collection">Part Collection</a></h6>
+
</div> <!-- /.sidebar-widget -->
−
<ul id="flickr-feed" class="thumbs"></ul>
+
−
</div> <!-- /.sidebar-widget -->
+
 
<div class="sidebar-widget">
 
<div class="sidebar-widget">
−
<img src="https://static.igem.org/mediawiki/2015/f/f2/Nankai_projectpic3.JPG">
+
<img src="https://static.igem.org/mediawiki/2015/d/de/Partsfigure_new1.jpeg">
−
                                                 <p>Preparing for LB medium.</p>
+
                                                 <p>Have fun in iGEM 2015!</p>
−
<img src="https://static.igem.org/mediawiki/2015/6/6e/Nankai_projectpic1.JPG">
+
<img src="https://static.igem.org/mediawiki/2015/4/40/Partsfigure_new2.jpeg">
−
                                                <p>Cultured LL3.</p>
+
                      <p>Have fun in iGEM 2015!</p>
−
<img src="https://static.igem.org/mediawiki/2015/2/2d/Nankai_projectpic2.jpg">
+
<img src="https://static.igem.org/mediawiki/2015/a/ad/Partsfigure_new3.jpeg">
−
                                                <p>In the progress of fermentation.</p>
+
                      <p>Have fun in iGEM 2015!</p>
  
 
</div> <!-- /.sidebar-widget -->
 
</div> <!-- /.sidebar-widget -->
−
<h6>References</h6>
+
<h6>&nbsp;</h6>
−
<p>1. Ashiuchi, M., Misono, H., 2002. Biochemistry and molecular genetics of poly-γ-glutamate synthesis. Appl. Biochem. Biotechnol. 59, 9–14.</br>
+
−
2. Kunioka, M., 1997. Biosynthesis and chemical reactions of poly(amino acid)s from
+
−
microorganisms. Appl. Microbiol. Biotechnol. 47, 469–475.</br>
+
−
3. Shih, I.L., Van, Y.T., 2001. The production of poly(γ-glutamic acid) from microorganism and its various applications. Bioresour. Technol. 79, 207–225.</br>
+
−
4. Li, C., 2002. Poly(L-glutamic acid)--anticancer drug conjugates. Adv. Drug Deliver. Rev. 54, 695–713.</br>
+
−
5. Liang, H.F., Chen, C.T., Chen, S.C., Kulkarni, A.R., Chiu, Y.L., Chen, M.C., Sung, H.W., 2006. Paclitaxel-loaded poly(γ-glutamic acid)-poly(lactide) nanoparticles as a targeted drug delivery system for the treatment of liver cancer. Biomaterials. 27, 2051–2059.</br>
+
−
6. Richard, A., Margaritis, A., 2001. Poly (glutamic acid) for biomedical applications. Crit. Rev. Biotechnol. 21, 219–232.</br>
+
−
7. Park, Y.J., Liang, J., Yang, Z., Yang, V.C., 2001. Controlled release of clot-dissolving tissue-type plasmmogen activator from a poly(L-glutamic acid) semi-interpenetrating polymer network hydrogel. J. Control. Release. 74, 243–247.</br>
+
−
8. Cao, M.F., Geng, W.T., Liu, L., Song, C.J., Xie, H., Guo, W.B., Jin, Y.H., Wang, S.F., 2011. Glutamic acid independent production of poly-γ-glutamic acid by Bacillus amyloliquefaciens LL3 and cloning of pgsBCA genes. Bioresour. Technol. 102, 4251–4257.</br>
+
−
9. Geng, W.T., Cao, M.F., Song, C.J., Xie, H., Liu, L., Yang, C., Feng, J., Zhang, W., Jin, Y.H., Du, Y., Wang, S.F., 2011. Complete genome sequence of Bacillus amyloliquefaciens LL3, which exhibits glutamic acid-independent production of poly-γ-glutamic acid. J. Bacteriol. 193, 3393–3394.</br>
+
−
10. Feng, J., Gao, W.X., Gu, Y.Y., Zhang, W., Cao, M.F., Song, C.J., Zhang, P., Sun, M., Yang, C.,  Wang, S.F., 2014a. Functions of poly-gamma-glutamic acid (γ-PGA) degradation genes in γ-PGA synthesis and cell morphology maintenance. Appl. Microbiol. Biotechnol. 98, 6397–6407.</br>
+
−
11. Uy, D., Delaunay S., Germain, P., Engasser, J.M., Goergen, J.L. 2003. Instability of glutamate production by Corynebacterium glutamicum 2262 in continuous culture using the temperature-triggered process. J. Biotech. 104, 173-184.</p>
+
 
</div> <!-- /.sidebar -->
 
</div> <!-- /.sidebar -->
 
</div> <!-- /.col-md-4 -->
 
</div> <!-- /.col-md-4 -->
Line 142: Line 194:
  
 
<!-- Scripts -->
 
<!-- Scripts -->
 +
<p class="tail">&nbsp;<p>
 
</html>
 
</html>
 
{{Nankaifoot}}
 
{{Nankaifoot}}

Latest revision as of 21:51, 18 September 2015

Medigo Blue, free responsive template

Your place: Home > Parts

Team Parts

Parts Abstract

Number Name Type Description Creator Length
BBa_K1628001 Pbca Promoter an original promoter of coding sequence pgsBCA operon Tianyi Huang 364
BBa_K1628002 Pxyl Promoter a promoter of xylose operon Tianyi Huang 217
BBa_K1628003 BJ27UP Promoter an artificially synthesized promoter Xinhao Song 100
BBa_K1628004 C2up Promoter an artificially synthesized promoter Xinhao Song 108
BBa_K1628005 A2up Promoter an artificially synthesized promoter Yibing Wei 107
BBa_K1628006 P43 Promoter a strong promoter in Bacillus subtilis 168 Yibing Wei 445
BBa_K1628007 PamyA Promoter a strong promoter in Bacillus amyloliquefaciens LL3 Zhaoran Zhang 613
BBa_K1628101 pgsB Coding a gene responsible for γ-PGA synthesis in pgsBCA operon Tianyi Huang 1182
BBa_K1628102 pgsCA Coding a coding gene in pgsBCA operon Xinhao Song 1631
BBa_K1628201 PlacI/lacI Translational Unit a promoter of lactose operon along with a repressor of lactose operon regulating the promoter Yibing Wei 1386
BBa_K1628202 Pgrac Promoter a promoter of lactose operon regulated by repressor LacI Tianyi Huang 120
BBa_K1628203 xylR Coding a repressor of xylose operon regulating promoter Pxyl Zhaoran Zhang 1167
BBa_K1628301 P1-GFP Device P3-GFP is a composite part used for the Measurement track of this year. This part consists of the promoter part of BBa_J23117, the official GFP device (with an RBS, GFP coding sequence and a set of double terminators) and a pSB1C3 backbone. We transformed this plasmid into E. coli cells and the fluorescence intensity of the GFP protein that was expressed by the bacteria was measured afterwards by a flow cytometry. Tianyi Huang ?
BBa_K1628202 P2-GFP Device P2-GFP is a composite part used for the Measurement track of this year. This part consists of the promoter part of BBa_J23106, the official GFP device (with an RBS, GFP coding sequence and a set of double terminators) and a pSB1C3 backbone. We transformed this plasmid into E. coli cells and the fluorescence intensity of the GFP protein that was expressed by the bacteria was measured afterwards by a flow cytometry. Xinhao Song ?
BBa_K1628303 P3-GFP Device P3-GFP is a composite part used for the Measurement track of this year. This part consists of the promoter part of BBa_J23117, the official GFP device (with an RBS, GFP coding sequence and a set of double terminators) and a pSB1C3 backbone. We transformed this plasmid into E. coli cells and the fluorescence intensity of the GFP protein that was expressed by the bacteria was measured afterwards by a flow cytometry. Yibing Wei ?
Team Parts
Basic Parts
Composite Parts

Have fun in iGEM 2015!

Have fun in iGEM 2015!

Have fun in iGEM 2015!

 

 

Medigo Blue, free responsive template

Copyright © Nankai University

Nankai IGEM Team 2015