Difference between revisions of "Team:Oxford/Notebook/Week1"

Revision as of 10:58, 2 July 2015

Date

Researcher(s)

Content

22/06/2015

Whole Team
(Introductory Session)

The gBlocks ordered from IDT arrived in the form of vials of 200ng solid DNA powder.

(refer to BioBricks page for information on DNA sequences)

The gBlocks were made into 10ng/µl stock solutions in Milli-Q water for storage:

mass / ng	conc / ngµl^-1	final volume / µl
200	10	20

The forward and reverse primers ordered from IDT came in 32.4nmol and 34.3nmol of solid respectively.

(Sequences: Forward - CTTTTTTGCCGGACTGC; Reverse - ATGATTTCTGGAATTCGC)

The primers were made into 100µM stock solutions in Milli-Q water for storage:

amt / 10^-9 mol	conc / 10^-6 M	final volume / 10^-6 L
32.4	100	324
34.3	100	343

@@ Line 115: / Line 115: @@
    <tr>
      <td>22/06/2015</td>
-     <td>Whole Team</td>
+     <td><p>Whole Team<br>
+                  (Introductory Session)</td>
      <td>
        <h4>Preparation of Stock Solutions</h4><a name="2206a"></a></h4>
@@ Line 126: / Line 127: @@
          <tr>
            <th>mass / ng</th>
-           <th>conc / ngµl-1</th>
+           <th>conc / ngµl<sup>-1</sup></th>
            <th>final volume / µl</th>
          <tr>
@@ Line 141: / Line 142: @@
        <table>
          <tr>
-           <th>amt / 10-9 mol</th>
+           <th>amt / 10<sup>-9</sup> mol</th>
-           <th>conc / 10-6 M</th>
+           <th>conc / 10<sup>-6</sup> M</th>
-           <th>final volume / 10-6 L</th>
+           <th>final volume / 10<sup>-6</sup> L</th>
          <tr>
          <tr>