|
|
| Line 1: |
Line 1: |
| − | {{:Team:Oxford/Templates/Nav}}
| |
| − | <html>
| |
| | | | |
| − | <div class="container-fluid page-heading">
| |
| − | <h3>Interlab Study</h3>
| |
| − | </div>
| |
| − |
| |
| − | <div class="container-fluid content-container">
| |
| − | <div class="row">
| |
| − | <div class="col-md-9">
| |
| − | <div class="slim">
| |
| − | <div class="section" id="Introduction">
| |
| − | <h2>Introduction</h2>
| |
| − | <div class="section" id="Overview">
| |
| − | <h3>Overview</h3>
| |
| − | <p>
| |
| − | One of the fundamental principles of synthetic biology is the characterization of standard biological parts. iGEM HQ's
| |
| − | International InterLab Measurement Study is an endeavour at achieving the large-scale characterization of a set of
| |
| − | biological devices which has been previously designed and catalogued in the BioBricks Registry. In this study, iGEM teams
| |
| − | from across the world set out to measure the fluorescence of model organisms which have been transformed with devices
| |
| − | comprising the same green fluorescent protein (GFP) gene expressed at different levels by being fused to promoters of
| |
| − | different strengths. This would provide a large dataset from which a more accurate analysis that accounts for lab-to-lab
| |
| − | variations can be drawn.
| |
| − | </p>
| |
| − | <p>
| |
| − | The promoters characterized in the 2015 InterLab Study are three constitutive promoters of different strengths found
| |
| − | within the <a href="http://parts.igem.org/Promoters/Catalog/Anderson">Anderson Promoter Collection</a>, which were included
| |
| − | in the 2015 iGEM Distribution Kit. We assembled each of these three promoters upstream of a given GFP-encoding BioBrick and
| |
| − | transformed each of the composite parts into three different strains of E. coli to measure their respective fluorescence levels.
| |
| − | We measured fluorescence levels using a 96-well microplate reader, a flow cytometer, as well as a confocal microscope.
| |
| − | </p>
| |
| − | </div>
| |
| − | <div class"section" id="KeyFindings">
| |
| − | <h3>Key Results and Findings</h3>
| |
| − | <p>
| |
| − | Through the data obtained from our microplate reader, we found that the strengths of the three promoters investigated are in the same order as Anderson et al's initial characterization
| |
| − | across the three E. coli strains which we studied. The relative fluorescence magnitudes we measured however do not agree with
| |
| − | the ratios presented in the Anderson study.
| |
| − | </p>
| |
| − | <p>
| |
| − | To eliminate the dependence on equipment parameters associated with arbitrary fluorescence units, we calibrated our fluorescence
| |
| − | readings against different concentrations of a fluorescent chemical standard, sodium fluorescein, and expressed fluorescence
| |
| − | intensity in units of its concentration.
| |
| − | </p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="col-md-3 contents-sidebar">
| |
| − | <ul id="sidebar" class="nav nav-stacked" data-spy="affix">
| |
| − | <li>
| |
| − | <a href="#Introduction">Introduction</a>
| |
| − | <ul class="nav nav-stacked">
| |
| − | <li><a href="#Overview">Overview</a></li>
| |
| − | <li><a href="#KeyFindings">Key Results and Findings</a></li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </html>
| |
| − | {{:Team:Oxford/Templates/Foot}}
| |