Difference between revisions of "Team:Pasteur Paris/Parts"

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<p> Page under construction </p>
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<h1>Parts</h1>
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<h3>BBa_K1622000</h3>
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<h5>Part Composition<h5>
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<p>This part is composed of 3 subparts :
 +
<ul>
 +
  <il>RBS: Ribosome Binding Site</il>
 +
  <il>Glycoaldehyde Dehydrogenase</il>
 +
  <il>Glycoaldehyde Reductase</il>
 +
 
 +
<h5>Glycoaldehyde Dehydrogenase</h5>
 +
<p>This enzyme catalyses the Break-down of Glycoaldehyde into Glycolate, which is the third reaction of our PET degradation chain.
 +
We obtained the original sequence of this subpart in the iGEM registry of standard parts (BBa_936011). We optimized our sequence for <i>E.coli<i> and ordered the synthesis from our sequence.</p>
 +
 
 +
<h5> Glycoaldehyde Reductase</h5>
 +
<p> The ethylene glycol to glycoaldehyde reaction is catalyzed by this enzyme. In the same manner as the Glycoaldehyde Dehydrogenase, we got the original sequence on the registry (BBa_K936023), optimized those sequences for <i>E.coli</i> and ordered their synthesis.</p>
 +
 
 +
<h5>Part Assembly</h5>
 +
<p> The subparts were assembled using Gibson Assembly. Prior to the actual assembly, we had to amplify our sequences via PCR using primers with extensions that contain overlapping sequences. </p>
 +
 
 +
<h3>BBa_K1622001</h3>
 +
 
 +
<h5>Part Composition<h5>
 +
<p>This part is a composite part composed of 2 subparts :
 +
<ul>
 +
  <il></il>
 +
  <il></il>
 +
  <il></il>
 +
 
 +
<h5>Glycoaldehyde Dehydrogenase</h5>
 +
<p>This enzyme catalyses the Break-down of Glycoaldehyde into Glycolate, which is the third reaction of our PET degradation chain.
 +
We obtained the original sequence of this subpart in the iGEM registry of standard parts (BBa_936011). We optimized our sequence for <i>E.coli<i> and ordered the synthesis from our sequence.</p>
 +
 
 +
<h5> Glycoaldehyde Reductase</h5>
 +
<p> The ethylene glycol to glycoaldehyde reaction is catalyzed by this enzyme. In the same manner as the Glycoaldehyde Dehydrogenase, we got the original sequence on the registry (BBa_K936023), optimized those sequences for <i>E.coli</i> and ordered their synthesis.</p>
 +
 
 +
<h5>Part Assembly</h5>
 +
<p> The subparts were assembled using Gibson Assembly. Prior to the actual assembly, we had to amplify our sequences via PCR using primers with extensions that contain overlapping sequences. </p>
  
 
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Revision as of 01:37, 19 September 2015

Parts

BBa_K1622000

Part Composition

This part is composed of 3 subparts :

    RBS: Ribosome Binding Site Glycoaldehyde Dehydrogenase Glycoaldehyde Reductase
    Glycoaldehyde Dehydrogenase

    This enzyme catalyses the Break-down of Glycoaldehyde into Glycolate, which is the third reaction of our PET degradation chain. We obtained the original sequence of this subpart in the iGEM registry of standard parts (BBa_936011). We optimized our sequence for E.coli and ordered the synthesis from our sequence.

    Glycoaldehyde Reductase

    The ethylene glycol to glycoaldehyde reaction is catalyzed by this enzyme. In the same manner as the Glycoaldehyde Dehydrogenase, we got the original sequence on the registry (BBa_K936023), optimized those sequences for E.coli and ordered their synthesis.

    Part Assembly

    The subparts were assembled using Gibson Assembly. Prior to the actual assembly, we had to amplify our sequences via PCR using primers with extensions that contain overlapping sequences.

    BBa_K1622001

    Part Composition

    This part is a composite part composed of 2 subparts :

      Glycoaldehyde Dehydrogenase

      This enzyme catalyses the Break-down of Glycoaldehyde into Glycolate, which is the third reaction of our PET degradation chain. We obtained the original sequence of this subpart in the iGEM registry of standard parts (BBa_936011). We optimized our sequence for E.coli and ordered the synthesis from our sequence.

      Glycoaldehyde Reductase

      The ethylene glycol to glycoaldehyde reaction is catalyzed by this enzyme. In the same manner as the Glycoaldehyde Dehydrogenase, we got the original sequence on the registry (BBa_K936023), optimized those sequences for E.coli and ordered their synthesis.

      Part Assembly

      The subparts were assembled using Gibson Assembly. Prior to the actual assembly, we had to amplify our sequences via PCR using primers with extensions that contain overlapping sequences.

      ^
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