Team:RHIT

Welcome to Rose-Hulman's MitochONdriOFF project!

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Our project focuses on controlling aerobic respiration in the yeast Saccharomyces cerevisiae. We will address this objective by regulating the expression of mitochondrial ribosomal protein MRPS12. This protein is essential to the function of mitochondrial ribosomes, which produce key components of the electron transport chain. Without these components, the electron transport chain is disabled and aerobic respiration is not possible.

Our project used yeast strains that had their endogenous MRPS12 gene disrupted with a kanamycin gene. We showed that when the MRPS12 gene was reintroduced to this strain on a plasmid, ETC function was restored.

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