Team:SDU-Denmark/Tour12

"The next best thing to knowing something is knowing where to find it." - Samuel Johnson

Attributions

Despite our tireless efforts we could not have done this on our own. Therefore we would like to express our gratitude and thank all.

Sponsors

Laboratory support

  • Our instructors Postdoc and Ph. D. Mikkel Girke Jørgensen, Academic Assistant Tina Kronborg, Ph. D. student Thøger Jensen Krogh and Stud.cand.scient Patrick Rosendahl Andreassen
  • Medical Laboratory Technician Simon Rose who provided a security course
  • Postdoc Anders Boysen helped with troubleshooting in the lab
  • Thermo Fisher Scientific

General support

  • DTU iGEM team – for arranging the anually bio brick workshop
  • Vilnius iGEM team for hosting our visit for three days
  • Uppsala iGEM team for arranging and hosting the Nordic Meetup
  • Associate professor, MD Søren Thue Lillevang, Department of Clinical Immunology, Odense University Hospital (OUH). For attributing to the discussion about medical aspects
  • Associate Professor Jan Nehlin, Department of Clinical Immunology, Odense University Hospital (OUH) for discussing patents and medical aspects, and suggestions to future work
  • Nancy Rasmussen for helping us proof reading our wiki
  • Members of the previous SDU-Denmark iGEM teams for giving feedback on our presentation and cooking for us
  • Robert William Naur for proof reading on The Little Curios Story about the Curios Little Being
  • Anna Valgreen for providing the illustrations, The Curios Little Story about a Curious Little Being

Technical support

  • Ph. D. Thøger Jensen Krogh helped us with any problem regarding our wiki site.
  • The SDU iGEM team 2013 for helping us with our wiki.

Litterature support

Antibody issue

  • Lubert Stryer JMB, John L. Tymoczko. The Immune System. Biochemistry. 7 ed: W. H. Freeman and Company; 2012.
  • Cowan MK. Host Defenses II - Specific Immunity and Immunization. Microbiology - A Systems Approach. 3 ed: McGraw-Hill; 2012.
  • Chippaux JP, Goyffon M. Venoms, antivenoms and immunotherapy. Toxicon. 1998;36(6):823-46.
  • Clem AS. Fundamentals of vaccine immunology. J Glob Infect Dis. 2011;3(1):73-8.
  • Siddiqui MZ. Monoclonal antibodies as diagnostics; an appraisal. Indian J Pharm Sci. 2010;72(1):12-7.
  • Berger M, Shankar V, Vafai A. Therapeutic applications of monoclonal antibodies. Am J Med Sci. 2002;324(1):14-30.
  • Scott AM, Wolchok JD, Old LJ. Antibody therapy of cancer. Nat Rev Cancer. 2012;12(4):278-87.
  • BioPharma P. What Are Humanized Monoclonal Antibodies 2015 [cited 2015 08/08]. (Link)
  • Payne WJ, Jr., Marshall DL, Shockley RK, Martin WJ. Clinical laboratory applications of monoclonal antibodies. Clin Microbiol Rev. 1988;1(3):313-29.
  • Hnasko RM, Stanker LH. Hybridoma Technology. Methods Mol Biol. 2015;1318:15-28.
  • Stills HF, Jr. Adjuvants and antibody production: dispelling the myths associated with Freund's complete and other adjuvants. ILAR journal / National Research Council, Institute of Laboratory Animal Resources. 2005;46(3):280-93.
  • Chames P, Van Regenmortel M, Weiss E, Baty D. Therapeutic antibodies: successes, limitations and hopes for the future. Br J Pharmocol. 2009;157(2):220-33.
  • Descartes Rene. Les Méditations métaphysiques’, 1647
  • Bentham Jeremy, Introduction to the Principles of Morals and Legislation, 1789
  • Three RS (Link)
  • Cruelty Free International (Link)
  • Nelson PN, Reynolds GM, Waldron EE, Ward E, Giannopoulos K, Murray PG. Demystified …: Monoclonal antibodies. Molecular Pathology. 2000;53(3):111-7.
  • Colas P. The eleven-year switch of peptide aptamers. Journal of biology. 2008;7(1):2.
  • Colas P, Cohen B, Jessen T, Grishina I, McCoy J, Brent R. Genetic selection of peptide aptamers that recognize and inhibit cyclin-dependent kinase 2. Nature. 1996;380(6574):548-50.
  • Borghouts C, Kunz C, Delis N, Groner B. Monomeric recombinant peptide aptamers are required for efficient intracellular uptake and target inhibition. Molecular cancer research : MCR. 2008;6(2):267-81.
  • Evans D, Johnson S, Laurenson S, Davies AG, Ko Ferrigno P, Wälti C. Electrical protein detection in cell lysates using high-density peptide-aptamer microarrays. Journal of biology. 2008;7(1):3.
  • Li J, Tan S, Chen X, Zhang CY, Zhang Y. Peptide aptamers with biological and therapeutic applications. Current medicinal chemistry. 2011;18(27):4215-22.
  • Swiss Institute of Bioinformatics. 2015. (Link)
  • Key Benefits: Affimers have some simple but essential advantages over antibodies: Avacta Life Sciences; [updated 19-08-2015]. (Link)

P.A.S.T

  • Karimova G, Pidoux J, Ullmann A, Ladant D. A bacterial two-hybrid system based on a reconstituted signal transduction pathway. Proceedings of the National Academy of Sciences of the United States of America. 1998;95(10):5752-6.(Link)
  • Battesti A, Bouveret E. The bacterial two-hybrid system based on adenylate cyclase reconstitution in Escherichia coli. Methods (San Diego, Calif). 2012;58(4):325-34.(Link)
  • SIMON L. DOVE, J. KEITH JOUNG* & ANN HOCHSCHILD - ‘Activation of prokaryotic transcription through arbitrary protein–protein contacts’ - Nature 386, 627-630 (10 April 1997).(Link)
  • J. Keith Joung, Elizabeth I. Ramm, and Carl O. Pabo - ‘A bacterial two-hybrid selection system for studying protein–DNA and protein–protein interactions’ - Proceedings of the National Academy of Science of the United States. vol. 97, (20 june 2000).(Link)

Process

  • World health organization, third edition, 2004 - Visited: 16.09.15.(Link)
  • Lov om miljø og genteknologi - Visited: 16.09.15.(Link)
  • Arbejdstilsynet - Visited: 16.09.15.(Link)
  • E.coli Genome Project, University of Wisconsin - Visited: 16.09.15.(Link)
  • NIH Guidelines for research involving - Visited: 16.09.15.(Link)
  • WO 2001073108 A2 - Patent - Visited: 16.09.15.(Link)
  • Europa-parlementets og rådets direktiv 2009/41/EF - Visited: 16.09.15.(Link)

Results

Practices

What's Next

  • Karimova G, Pidoux J, Ullmann A, Ladant D. - A bacterial two-hybrid system based on a reconstituted signal transduction pathway. Proceedings of the National Academy of Sciences of the United States of America. 1998;95(10):5752-6.(Link)