Team:SDU-Denmark/Tour12

"The next best thing to knowing something is knowing where to find it." - Samuel Johnson

Attributions

Despite our tireless efforts we could not have done this on our own. Therefore we would like to express our gratitude and thank all.

Sponsors

Laboratory support

  • Our instructors Postdoc and Ph. D. Mikkel Girke Jørgensen, Academic Assistant Tina Kronborg, Ph. D. student Thøger Jensen Krogh and Stud.cand.scient Patrick Rosendahl Andreassen
  • Medical Laboratory Technician Simon Rose who provided a security course
  • Postdoc Anders Boysen helped with troubleshooting in the lab

General support

  • DTU iGEM team – for arranging the anually bio brick workshop
  • Vilnius iGEM team for hosting our visit for three days
  • Uppsala iGEM team for arranging and hosting the Nordic Meetup
  • Associate Professor, MD Søren Thue Lillevang, Department of Clinical Immunology, Odense University Hospital, for attributing to the discussion about medical aspects
  • Associate Professor Jan Nehlin, Department of Clinical Immunology, Odense University Hospital, for discussing patents and medical aspects, and suggestions to future work
  • Nancy Rasmussen for helping us proof reading our wiki
  • Members of the previous SDU-Denmark iGEM teams for giving feedback on our presentation and cooking for us
  • Robert William Naur for proof reading on The Little Curios Story about the Curios Little Being
  • Anna Valgreen for providing the illustrations, The Curios Little Story about a Curious Little Being
  • Torben Hansson, Associated Professor, Institude of Chemical Engineering, Biotechnology and Enviromental Technology - for helping us with the Process Flow Diagram.
  • Knud Villy Christensen, Associate Professor, MSc. Chem. Eng. PhD in Chem. Eng. - for helping advice on the Process Flow Diagram.

Technical support

  • Ph. D. Thøger Jensen Krogh helped us with any problem regarding our wiki site.
  • The SDU iGEM team 2013 for helping us with our wiki.

Litterature support

Antibody issue

  • Liu JK1 (2014) The history of monoclonal antibody development - Progress, remaining challenges and future innovations. Sep 11;3(4):113-6. DOI: 10.1016/j.amsu.2014.09.001. (LINK)
  • Dawn M Ecker, Susan Dana Jones & Howard L Levine (2015) The therapeutic monoclonal antibody market, mAbs, 7:1, 9-14, DOI: 10.4161/19420862.2015.989042. (LINK)
  • Lubert Stryer JMB, John L. Tymoczko. The Immune System. Biochemistry. 7 ed: W. H. Freeman and Company; 2012.
  • Cowan MK. Host Defenses II - Specific Immunity and Immunization. Microbiology - A Systems Approach. 3 ed: McGraw- Hill; 2012.
  • Chippaux JP, Goyffon M. Venoms, antivenoms and immunotherapy. Toxicon. 1998;36(6):823-46.
  • Clem AS. Fundamentals of vaccine immunology. J Glob Infect Dis. 2011;3(1):73-8.
  • Siddiqui MZ. Monoclonal antibodies as diagnostics; an appraisal. Indian J Pharm Sci. 2010;72(1):12-7.
  • Berger M, Shankar V, Vafai A. Therapeutic applications of monoclonal antibodies. Am J Med Sci. 2002;324(1):14-30.
  • Payne WJ, Jr., Marshall DL, Shockley RK, Martin WJ. Clinical laboratory applications of monoclonal antibodies. Clin Microbiol Rev. 1988;1(3):313-29.
  • Hnasko RM, Stanker LH. Hybridoma Technology. Methods Mol Biol. 2015;1318:15-28.
  • Stills HF, Jr. Adjuvants and antibody production: dispelling the myths associated with Freund's complete and other adjuvants. ILAR journal / National Research Council, Institute of Laboratory Animal Resources. 2005;46(3):280-93.
  • Rene Descartes, Meditationer over den første filosofi. Publisher: Det Lille Forlag 1. edition, published 19.04.2002.
  • Jeremy Bentham, An Introduction to the Principles of Morals and Legislation (General Historical Collections). Publisher: British Library, Historical Print Editions. Published 25.03.2011
  • Good Animal Practise in Science. Visited: 04.09.15. (LINK)
  • Cruelty Free International. Visited 04.09.15. (LINK)
  • Colas P. The eleven-year switch of peptide aptamers. Journal of biology. 2008;7(1):2.
  • Borghouts C, Kunz C, Delis N, Groner B. Monomeric recombinant peptide aptamers are required for efficient intracellular uptake and target inhibition. Molecular cancer research : MCR. 2008;6(2):267-81.
  • Evans D, Johnson S, Laurenson S, Davies AG, Ko Ferrigno P, Wälti C. Electrical protein detection in cell lysates using high-density peptide-aptamer microarrays. Journal of biology. 2008;7(1):3.
  • Li J, Tan S, Chen X, Zhang CY, Zhang Y. Peptide aptamers with biological and therapeutic applications. Current medicinal chemistry. 2011;18(27):4215-22.
  • Swiss Institute of Bioinformatics. 2015. (LINK)
  • Key Benefits: Affimers have some simple but essential advantages over antibodies: Avacta Life Sciences; [updated 19- 08-2015]. (LINK)

PAST

  • Karimova G, Pidoux J, Ullmann A, Ladant D. (1998) A bacterial two-hybrid system based on a reconstituted signal transduction pathway. 1998;95(10):5752-6. (LINK)
  • Battesti A, Bouveret E. (2012) The bacterial two-hybrid system based on adenylate cyclase reconstitution in Escherichia coli. 2012;58(4):325-34. DOI:10.1016/j.ymeth.2012.07.018. (LINK)
  • SIMON L. DOVE, J. KEITH JOUNG* & ANN HOCHSCHILD. (1997) Activation of prokaryotic transcription through arbitrary protein–protein contacts. Nature 386, 627-630 (10 April 1997). (LINK)
  • J. Keith Joung, Elizabeth I. Ramm, and Carl O. Pabo (2000) A bacterial two-hybrid selection system for studying protein–DNA and protein–protein interactions. PNAS. US. vol. 97, (20 june 2000). (LINK)
  • Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. (LINK)
  • Borghouts C, Kunz C, Delis N, Groner B. Monomeric Recombinant Peptide Aptamers Are Required for Efficient Intracellular Uptake and Target Inhibition. Molecular Cancer Research. 2008;6(2):267-81. (LINK)
  • Sigmar-Aldrich: 3xFLAG system. Visited: 16.09.15. (LINK)
  • Seider, Seader, Lewin and Widagoo. Product and Process Design Principles - Synthesis, Analysis, and Evaluation; Third Edition - International Student Version; John Wiley & Sons, Inc.; 2010
  • Available at: https://www.sigmaaldrich.com/denmark.html [accessed: 10.09.2015]
  • Available at: https://www.neb.com/products/e8036-chitin-magnetic-beads [accessed: 13.09.2015]
  • Available at: http://www.rand.org/content/dam/rand/pubs/monograph_reports/MR1325/MR1325.ch9.pdf [accessed: 15.09.2015]
  • Sigma-Aldrich. Available at: http://www.sigmaaldrich.com/catalog/product/sigma/a2220?lang=en&region=DK [Accessed 1st august 2015]
  • L. Yee and H. W. Blanch. Recombinant Trypsin Production in High Cell Density Fed-Batch Cultures in Escherichia coli. Biotechnology and Bioengineering. 1993; 41: 781-790
  • Michael L. Shuler and Fikret Kargi. Bioprocess Engineering - Basic Concepts; second edition; Pearson Education International; 2002
  • IMPACT™ Kit. 2015. New England Biolabs; [accessed 2015 Sep 11] (LINK)
  • Pharmacia. Introduction to Expanded Bed Adsorption. (LINK)
  • Sigma-Aldrich. (LINK)
  • jeffrey Fu, David B. Wilson and Michael L. Shuler. Continuous, High Level Production and Excretion of a Plasmid- Encoded Protein by Escherichia coli in a Two-Stage Chemostat. Biotechnology and Bioengineering, 1993; 41: 937-946

Process

  • World health organization, third edition, 2004 - Visited: 16.09.15. (LINK)
  • Arbejdstilsynet - Visited: 16.09.15. (LINK)
  • Lov om miljø og genteknologi - Visited: 16.09.15. (LINK)
  • E.coli Genome Project, University of Wisconsin - Visited: 16.09.15. (LINK)
  • WO 2001073108 A2 - Patent - Visited: 16.09.15. (LINK)
  • The EU-parliaments directive 2009/41/EF - Visited: 16.09.15. (LINK)

Results

  • One Shot® Top10 Chemically Competent E. coli. Catalog number: C4040-10. Visited: 17.09.15. (LINK)
  • Kate B. Bechtol, David K. Hanzel, and Bee Choo Liang, Amersham Biosciences, Inc. - Understanding Fluorescence. (LINK)
  • iGEM Parts Registry - BBa_J23119:Design. Visited: 17.09.15. (LINK)
  • JCAnderson. iGEM Parts Registry - Visited: 17.09.15. (LINK)
  • Brendan P. Cormack, Raphael H. Valdiviaa, Stanley Falkow - FACS-optimized mutants of the green fluorescent protein (GFP). DOI:10.1016/0378-1119(95)00685-0. (LINK)

Practices

  • Jürgen Habermas, Diskurs Etik. Publisher: Det Lille Forlag, 1. edition, published 02.09.1998.
    ISBN-13: 9788790030445

What's Next

  • Kim CS, et al. Cellular imaging of endosome entrapped small gold nanoparticles, 2015 Jun 10;2:306-15. doi: 10.1016/j.mex.2015.06.001. (LINK)
  • Thorek DL et al. Reverse-Contrast Imaging and Targeted Radiation Therapy of Advanced Pancreatic Cancer Models. Int J Radiat Oncol Biol Phys. 2015 Jun 9. pii: S0360-3016(15)00612-4. DOI:10.1016/j.ijrobp.2015.06.001. (LINK)
  • Wacker M et al. N-linked glycosylation in Campylobacter jejuni and its functional transfer into E. coli. Science. 2002 Nov 29;298(5599):1790-3. (LINK)
  • Silberstein S, Lenz R, Xu C. Therapeutic Monoclonal Antibodies: What Headache Specialists Need to Know. Headache. 2015 Aug 28. DOI: 10.1111/head.12642. (LINK)
  • Technical Guide for ELISA. Available from KPL, Kirkegaard & Perry Laboratories. (LINK)
  • An Introduction to BiaCore's SPR Technology. Available from BiaCore.com. (LINK)
  • Open WetWare contributors, "Beta-Galactosidase Assay (A better Miller)," Open WetWare27 August 2012, 23:20 UTC. (LINK)