Difference between revisions of "Team:SDU-Denmark/Tour41"
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<td><b>000</b></td> | <td><b>000</b></td> | ||
<td><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/e/e8/SDU_2015_Protocol_000_-_Template.pdf" target="_blank">Template</a></td> | <td><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/e/e8/SDU_2015_Protocol_000_-_Template.pdf" target="_blank">Template</a></td> | ||
− | <td></td> | + | <td>Template for all the protocols made by SDU iGEM 2015.</td> |
</tr> | </tr> | ||
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<td><b>001</b></td> | <td><b>001</b></td> | ||
<td><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/3/39/SDU_2015_Protocol_001_-_Linker_GFP.pdf" target="_blank">Linker GFP</a></td> | <td><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/3/39/SDU_2015_Protocol_001_-_Linker_GFP.pdf" target="_blank">Linker GFP</a></td> | ||
− | <td></td> | + | <td>Assembly of a biobrick that contains the Linker-GFP gene.</td> |
</tr> | </tr> | ||
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<td><b>002</b></td> | <td><b>002</b></td> | ||
<td><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/9/9a/SDU_2015_Protocol_002_-_Control_of_LeuZ.pdf" target="_blank">Control of Leucine Zipper</a></td> | <td><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/9/9a/SDU_2015_Protocol_002_-_Control_of_LeuZ.pdf" target="_blank">Control of Leucine Zipper</a></td> | ||
− | <td></td> | + | <td>To conjugate a leucin zipper to the T18 and T25 domain as used in the bacterial two-hybrid |
+ | system and to furthermore verify that the bacterial two-hybrid system works</td> | ||
</tr> | </tr> | ||
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<td><b>003</b></td> | <td><b>003</b></td> | ||
<td><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/5/5a/SDU_2015_Protocol_003_-_Interlab_Study.pdf" target="_blank">Interlab Study</a></td> | <td><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/5/5a/SDU_2015_Protocol_003_-_Interlab_Study.pdf" target="_blank">Interlab Study</a></td> | ||
− | <td></td> | + | <td>Measurement of GFP under influence of different promoters.</td> |
</tr> | </tr> | ||
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<td><b>004</b></td> | <td><b>004</b></td> | ||
<td><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/b/ba/SDU_2015_Protocol_004_-_cAMP_Indicator.pdf" target="_blank">cAMP Indicator</a></td> | <td><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/b/ba/SDU_2015_Protocol_004_-_cAMP_Indicator.pdf" target="_blank">cAMP Indicator</a></td> | ||
− | <td></td> | + | <td>To create a brick that can indicate the activation of CyaA in the two hybrid system, by colour |
− | + | expression when increased presence of cAMP.</td> | |
+ | </tr> | ||
<tr> | <tr> | ||
<td><b>005</b></td> | <td><b>005</b></td> | ||
<td><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/c/c5/SDU_2015_Protocol_005_-_CyaA_Knockout.pdf" target="_blank">CyaA Knockout</a></td> | <td><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/c/c5/SDU_2015_Protocol_005_-_CyaA_Knockout.pdf" target="_blank">CyaA Knockout</a></td> | ||
− | <td></td> | + | <td>To create a knockout of the CyaA gene in MG1655. This is done to get a bacterial strain which we |
+ | can use to use and test the Two-Hybrid System.</td> | ||
</tr> | </tr> | ||
Revision as of 23:44, 15 September 2015
Complete Journal
Underneath is the highlighted moments of this summer. All our successes and major events we attended this summer with the team. We also listed the most important iGEM dates as well as protocols and primer designs. Feel free to click through the boxes, check out our primer designs or read through the protocols for the Lab.
Major events
Month: |
Date: |
Event: |
Febuary | 23.02.15 | First meeting |
March | 20.03.15- 22.03.15 |
Trip to Langeland |
24.03.15 | Project idea chosen | |
25.03.15 | Visiting Knud Villy Christiansen on TEK | |
April | 01.04.15 | First day in Lab |
10.04.15 | CFO Easter Party | |
15.04.15 | Lasagna night | |
24.04.15- 26.04.15 |
Danish Meetup | |
July | 24.07.15- 26.07.15 |
Nordic Jamboree |
28.07.15 | Presentation for UNF-camp | |
August | 03.08.15 | Visiting animal testing facility |
05.08.15 | Wiki and Wine | |
September | 14.09.15 | iGEM Alumne - Dinner with the old iGEM members |
??.09.15 | Sleepover in the "Lab" | |
??.09.15 | Movie Night: Back to the Future! | |
19.09.15 | Wiki-freeze Brunch | |
19.09.15- 20.09.15 |
Wiki-freeze Afterparty |
Lab successes
Month: |
Date: |
Succes: |
April | ||
May | ||
June | ||
July | ||
August | ||
September |
iGEM deadlines
Month: |
Date: |
Deadline: |
March | 31.03.15 | Team registration closes |
July | 15.07.15 | Project Description |
31.07.15 | Giant Jamboree registration closes | |
August | 07.08.15 | Track Selection |
07.08.15 | Project title and abstract | |
28.08.15 | Interlab Study data due | |
September | 18.09.15 | Parts Submission |
18.09.15 | Wiki-freeze | |
24.09.15- 28.09.15 |
Giant Jamboree |
Protocols
Number: |
Name: |
Description |
000 | Template | Template for all the protocols made by SDU iGEM 2015. |
001 | Linker GFP | Assembly of a biobrick that contains the Linker-GFP gene. |
002 | Control of Leucine Zipper | To conjugate a leucin zipper to the T18 and T25 domain as used in the bacterial two-hybrid system and to furthermore verify that the bacterial two-hybrid system works |
003 | Interlab Study | Measurement of GFP under influence of different promoters. |
004 | cAMP Indicator | To create a brick that can indicate the activation of CyaA in the two hybrid system, by colour expression when increased presence of cAMP. |
005 | CyaA Knockout | To create a knockout of the CyaA gene in MG1655. This is done to get a bacterial strain which we can use to use and test the Two-Hybrid System. |
Primers
Number: |
Name: |
Sequence: |
001 | pKT25_BB_F | 5’-ATATGAATTCGCGGCCGCTTCTAGAGCTGGCACGACAGGTTTCCCG-3’ |
002 | pKT25_BB_R | 5’-ATATCTGCAGCGGCCGCTACTACTAGGATCCAGCCCGCCGCGT-3’ |
003 | pUT18_BB_R | 5’-ATATCTGCAGCGGCCGCTACTAGTAGGATCCGCGTTCCACTGCGCCC-3’ |
004 | Linker-GFP_F | 5’-CGCTTCTAGCGGGATCCGAAAATTTGTATTTTCAATCTGGTATGCGTAAAGGAGAAGAACTTTTCACT GG-3’ |
005 | Con-DsRed_F | 5’-CGCTTCTAGAGTTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCAAAGAGGAGAAATACTAGATGG ATAGCACTGAGAACGTCATCAAGC-3’ |
006 | Con-DsRed_R | 5’-ATATCTGCAGCGGCCGCTACTAGTAGCGAAAAAACCCCGCCGAAGCGGGGTTTTTTGCGTTATTACTG GAACAGGTGGTGGCGG-3’ |
007 | GFP-mCherry_R/ GFP-DsRed_R |
5’-GAGCTAGCCGTAAACTAGTATATAAACGCAGAAAGGCCCACCC-3’ |
008 | GFP-mCherry_F/ GFP-DsRed_F |
5’-CTGCGTTTATATACTAGTTTACGGCTAGCTCAGTCCTAGGG-3’ |
009 | Con-mCherry_R | 5’-ATATCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCC-3’ |
010 | Con-mCherry_F | 5’-CGCTTCTAGCGTTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCAAAGAGGAGAAATACTAGATGG TGAGCAAGG-3’ |
011 | cyaA_F | 5’-GAGGTCTGGCAGTGGATCCT-3’ |
012 | cyaA_R | 5’-GAGTGGATTTGTGATCGCAGCG-3’ |
013 | CyaA_del_F | 5’-GGAATTTACAGAGAATAAACGGTGCTACACTTGTATGTAGCGCATCTTTCTGTGTAGGCTGGAGCTGC TTC-3’ |
014 | cyaA_del_R | 5’-GTTTCCGCTAAGATTGCATGCCGGATAAGCCTCGCTTTCCGGCACGTTCACATATGAATATCCTCCTT AG-3’ |
015 | FWLeuZ-F | 5’-CCCCGGATCCAAACCGTTCCAGTGCCG-3’ |
016 | RSLeuZ-R | 5’-ATATCTGCAGCGGCCGCTACTAGTAGAGATCTACACTAGCACTATCAGAGTTATTAAGC-3’ |
017 | iGEM VF2 | 5’-CCACCTGACGTCTAAGAAAC-3’ |
018 | iGEM VR | 5’-ATTACCGCCTTTGAGTGAGC-3’ |
019 | Zip_F | 5’-CGCTTCTAGAGGGATCCGAAAATTTGTATTTTCAATCTGGTATGAAACAGCTGGAAGACAAAGTTGA-3’ |
020 | Zip_R | 5’-ATATCTGCAGCGGCCGCTACTAGTAACGTTCACCAACCAGTTTTTTCAGA-3’ |
021 | H1F_CyaA_del | 5’-GCATCCAGACTGTCATAGTTATTGGAC-3’ |
022 | H1R_CyaA_del | 5’-GAAAGATGCGCTACATACAAGTGTAGC-3’ |
023 | H2F_CyaA_del | 5’-TGAACGTGCCGGAAAGCGAG-3’ |
024 | H2R_CyaA_del | 5’-AAGTGAGGAAATGCTGCGAGTATAAC-3’ |
025 | GFP_LVA_F | 5’-GCGCCGTCTAGAAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACT-3’ |
026 | GFP_LVA_F | 5’-GCCGGACTGCAGCGGCCGCTACTAGTATTAAGCTACTAAAGCGTAGTTTTCGTCG-3’ |