Difference between revisions of "Team:SDU-Denmark/Tour41"
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<td><li>To create a knockout of the CyaA gene in MG1655. This is done to get a bacterial<br> strain which we | <td><li>To create a knockout of the CyaA gene in MG1655. This is done to get a bacterial<br> strain which we | ||
can use to use and test the Two-Hybrid System.</li></td> | can use to use and test the Two-Hybrid System.</li></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><b><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/d/dd/SDU_2015_Protocol_006_-_Characterization_of_PcstA_Promoter.pdf" target="_blank">006</a></b></td> | ||
+ | <td><b>Characterization of PcstA</b></td> | ||
+ | <td><li>To better characterize the cAMPinducable promotor PcstA. Three experiments are designed and executed in this protocol.</li></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><b><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/a/ac/SDU_2015_Protocol_007_-_Two-hybrid_system.pdf" target="_blank">007</a></b></td> | ||
+ | <td><b>The Two Hybrid System</b></td> | ||
+ | <td><li>To construct two bricks that interact with each other allowing CyaA activation. The activation | ||
+ | only occurs if the proteins bound to CyaA interact with each other. This interaction allows CyaA | ||
+ | to produce cAMP from ATP.</li></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><b><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/5/59/SDU_2015_Protocol_008_-_Peptide_Aptamer_Screening.pdf" target="_blank">008</a></b></td> | ||
+ | <td><b>Peptide Aptamer Screening</b></td> | ||
+ | <td><li>To screen for peptide aptamers using the twohybrid system</li></td> | ||
</tr> | </tr> | ||
Revision as of 00:46, 16 September 2015
Complete Journal
Underneath is the highlighted moments of this summer. All our successes and major events we attended this summer with the team. We also listed the most important iGEM dates as well as protocols and primer designs. Feel free to click through the boxes, check out our primer designs or read through the protocols for the Lab. The protocols can be accessed by clicking on the protocol number.
Major events
Month: |
Date: |
Event: |
Febuary | 23.02.15 | First meeting |
March | 20.03.15- 22.03.15 |
Trip to Langeland |
24.03.15 | Project idea chosen | |
25.03.15 | Visiting Knud Villy Christiansen on TEK | |
April | 01.04.15 | First day in Lab |
10.04.15 | CFO Easter Party | |
15.04.15 | Lasagna night | |
24.04.15- 26.04.15 |
Danish Meetup | |
July | 24.07.15- 26.07.15 |
Nordic Jamboree |
28.07.15 | Presentation for UNF-camp | |
August | 03.08.15 | Visiting animal testing facility |
05.08.15 | Wiki and Wine | |
September | 14.09.15 | iGEM Alumne - Dinner with the old iGEM members |
16.09.15 | Sleepover 1.0 in the "Lab" | |
??.09.15 | Movie Night: Back to the Future! | |
19.09.15 | Wiki-freeze Brunch | |
19.09.15- 20.09.15 |
Wiki-freeze Afterparty |
Lab successes
Month: |
Date: |
Succes: |
April | ||
May | ||
June | ||
July | ||
August | ||
September |
iGEM deadlines
Month: |
Date: |
Deadline: |
March | 31.03.15 | Team registration closes |
July | 15.07.15 | Project Description |
31.07.15 | Giant Jamboree registration closes | |
August | 07.08.15 | Track Selection |
07.08.15 | Project title and abstract | |
28.08.15 | Interlab Study data due | |
September | 18.09.15 | Parts Submission |
18.09.15 | Wiki-freeze | |
24.09.15- 28.09.15 |
Giant Jamboree |
Protocols
Number: |
Name: |
Description |
000 | Template | |
001 | Linker GFP | |
002 | Control of Leucine Zipper | two-hybrid system and to furthermore verify that the bacterial two-hybrid system works |
003 | Interlab Study | |
004 | cAMP Indicator | system, by colour expression when increased presence of cAMP. |
005 | CyaA Knockout | strain which we can use to use and test the Two-Hybrid System. |
006 | Characterization of PcstA | |
007 | The Two Hybrid System | |
008 | Peptide Aptamer Screening |
Primers
Number: |
Name: |
Sequence: |
001 | pKT25_BB_F | 5’-ATATGAATTCGCGGCCGCTTCTAGAGCTGGCACGACAGGTTTCCCG-3’ |
002 | pKT25_BB_R | 5’-ATATCTGCAGCGGCCGCTACTACTAGGATCCAGCCCGCCGCGT-3’ |
003 | pUT18_BB_R | 5’-ATATCTGCAGCGGCCGCTACTAGTAGGATCCGCGTTCCACTGCGCCC-3’ |
004 | Linker-GFP_F | 5’-CGCTTCTAGCGGGATCCGAAAATTTGTATTTTCAATCTGGTATGCGTAAAGGAGAAGAACTTTTCACT GG-3’ |
005 | Con-DsRed_F | 5’-CGCTTCTAGAGTTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCAAAGAGGAGAAATACTAGATGG ATAGCACTGAGAACGTCATCAAGC-3’ |
006 | Con-DsRed_R | 5’-ATATCTGCAGCGGCCGCTACTAGTAGCGAAAAAACCCCGCCGAAGCGGGGTTTTTTGCGTTATTACTG GAACAGGTGGTGGCGG-3’ |
007 | GFP-mCherry_R/ GFP-DsRed_R |
5’-GAGCTAGCCGTAAACTAGTATATAAACGCAGAAAGGCCCACCC-3’ |
008 | GFP-mCherry_F/ GFP-DsRed_F |
5’-CTGCGTTTATATACTAGTTTACGGCTAGCTCAGTCCTAGGG-3’ |
009 | Con-mCherry_R | 5’-ATATCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCC-3’ |
010 | Con-mCherry_F | 5’-CGCTTCTAGCGTTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCAAAGAGGAGAAATACTAGATGG TGAGCAAGG-3’ |
011 | cyaA_F | 5’-GAGGTCTGGCAGTGGATCCT-3’ |
012 | cyaA_R | 5’-GAGTGGATTTGTGATCGCAGCG-3’ |
013 | CyaA_del_F | 5’-GGAATTTACAGAGAATAAACGGTGCTACACTTGTATGTAGCGCATCTTTCTGTGTAGGCTGGAGCTGC TTC-3’ |
014 | cyaA_del_R | 5’-GTTTCCGCTAAGATTGCATGCCGGATAAGCCTCGCTTTCCGGCACGTTCACATATGAATATCCTCCTT AG-3’ |
015 | FWLeuZ-F | 5’-CCCCGGATCCAAACCGTTCCAGTGCCG-3’ |
016 | RSLeuZ-R | 5’-ATATCTGCAGCGGCCGCTACTAGTAGAGATCTACACTAGCACTATCAGAGTTATTAAGC-3’ |
017 | iGEM VF2 | 5’-CCACCTGACGTCTAAGAAAC-3’ |
018 | iGEM VR | 5’-ATTACCGCCTTTGAGTGAGC-3’ |
019 | Zip_F | 5’-CGCTTCTAGAGGGATCCGAAAATTTGTATTTTCAATCTGGTATGAAACAGCTGGAAGACAAAGTTGA-3’ |
020 | Zip_R | 5’-ATATCTGCAGCGGCCGCTACTAGTAACGTTCACCAACCAGTTTTTTCAGA-3’ |
021 | H1F_CyaA_del | 5’-GCATCCAGACTGTCATAGTTATTGGAC-3’ |
022 | H1R_CyaA_del | 5’-GAAAGATGCGCTACATACAAGTGTAGC-3’ |
023 | H2F_CyaA_del | 5’-TGAACGTGCCGGAAAGCGAG-3’ |
024 | H2R_CyaA_del | 5’-AAGTGAGGAAATGCTGCGAGTATAAC-3’ |
025 | GFP_LVA_F | 5’-GCGCCGTCTAGAAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACT-3’ |
026 | GFP_LVA_F | 5’-GCCGGACTGCAGCGGCCGCTACTAGTATTAAGCTACTAAAGCGTAGTTTTCGTCG-3’ |