Difference between revisions of "Team:San Andres/Experiments"

(Blanked the page)
 
(9 intermediate revisions by 2 users not shown)
Line 1: Line 1:
<html>
+
 
<head>
+
  <meta content="text/html; charset=ISO-8859-1"
+
http-equiv="content-type">
+
  <title></title>
+
</head>
+
<body>
+
<meta content="text/html; charset=ISO-8859-1"
+
http-equiv="content-type">
+
<title></title>
+
<img src="https://static.igem.org/mediawiki/2015/0/09/Firma_800.jpg"
+
gwd-img-541d="">
+
<title>wiki 2</title>
+
<meta http-equiv="Content-Type"
+
content="text/html; charset=utf-8">
+
<meta name="generator"
+
content="Google Web Designer 1.3.2.0521">
+
<style type="text/css">
+
html, body {
+
width: 100%;
+
height: 10%;
+
margin: 0px;
+
}
+
body {
+
-webkit-transform: perspective(1400px) matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1);
+
-webkit-transform-style: preserve-3d;
+
background-color: transparent;
+
}
+
.gwd-table-nb4m {
+
font-size: 24px;
+
-webkit-transform: translate3d(0px, -2px, 0px);
+
-webkit-transform-style: preserve-3d;
+
}
+
.gwd-td-b6db {
+
border: 1px solid black;
+
}
+
.gwd-a-g1jl {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-o5pu {
+
border: 1px solid black;
+
}
+
.gwd-a-7xj9 {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-jbkf {
+
border: 1px solid black;
+
}
+
.gwd-td-f88u {
+
border: 1px solid black;
+
}
+
.gwd-a-8khq {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-j5rf {
+
border: 1px solid black;
+
}
+
.gwd-a-50lt {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-3tnb {
+
border: 1px solid black;
+
}
+
.gwd-a-oa0r {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-ki1q {
+
border: 1px solid black;
+
}
+
.gwd-a-3fxh {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-0vlu {
+
border: 1px solid black;
+
}
+
.gwd-a-mwsi {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-zyck {
+
border: 1px solid black;
+
}
+
.gwd-a-j8kh {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-uz3t {
+
border: 1px solid black;
+
}
+
.gwd-a-83hm {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-fnbn {
+
border: 1px solid black;
+
}
+
.gwd-a-80zp {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-asn4 {
+
border: 1px solid black;
+
}
+
.gwd-a-chbv {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-8c55 {
+
border: 1px solid black;
+
}
+
.gwd-a-rf1i {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-jce5 {
+
border: 1px solid black;
+
}
+
.gwd-a-bm82 {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-7vtt {
+
border: 1px solid black;
+
}
+
.gwd-a-ffa8 {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-aqks {
+
border: 1px solid black;
+
}
+
.gwd-a-1thu {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-dgpg {
+
border: 1px solid black;
+
}
+
.gwd-a-cpqy {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-788u {
+
border: 1px solid black;
+
}
+
.gwd-a-z37b {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-div-2ed9 {
+
position: absolute;
+
width: 50px;
+
height: 31px;
+
left: 713px;
+
top: 92px;
+
font-family: 'Times New Roman';
+
text-align: left;
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-1it6 {
+
border: 2px solid black;
+
}
+
.gwd-a-cfz2 {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-gm83 {
+
border: 2px solid black;
+
}
+
.gwd-a-d494 {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-werd {
+
border: 2px solid black;
+
}
+
.gwd-a-feiv {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-pvfp {
+
border: 2px solid black;
+
}
+
.gwd-a-laum {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-yx5y {
+
border: 2px solid black;
+
}
+
.gwd-a-iekk {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-844w {
+
border: 2px solid black;
+
}
+
.gwd-a-njb2 {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-g48r {
+
border: 2px solid black;
+
}
+
.gwd-a-r862 {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-sdu6 {
+
border: 2px solid black;
+
}
+
.gwd-a-tjrm {
+
color: rgb(0, 0, 0);
+
}
+
.gwd-td-4dr4 {
+
border: 2px solid black;
+
}
+
.gwd-a-yt4x {
+
color: rgb(0, 0, 0);
+
}
+
</style><!--navigation menu -->
+
<table class="gwd-table-nb4m editable" width="100%">
+
  <tbody>
+
    <tr heigth="15px">
+
    </tr>
+
    <tr heigth="75px">
+
      <td style="border: 2px solid black;"
+
onmouseover="this.bgColor='#ffba00'"
+
onmouseout="this.bgColor='#fcff00'" align="center"
+
bgcolor="#fcff00" height="45">
+
      <a href="https://2015.igem.org/Team:San_Andres"
+
style="color: rgb(0, 0, 0);">Home </a> </td>
+
      <td style="border: 2px solid black;"
+
onmouseover="this.bgColor='#ffba00'"
+
onmouseout="this.bgColor='#fcff00'" align="center"
+
bgcolor="#fcff00" height="45">
+
      <a href="https://2015.igem.org/Team:San_Andres/Team"
+
style="color: rgb(0, 0, 0);"> Team </a> </td>
+
      <td style="border: 2px solid black;"
+
onmouseover="this.bgColor='#ffba00'"
+
onmouseout="this.bgColor='#fcff00'" align="center"
+
bgcolor="#fcff00" height="45">
+
      <a href="https://2015.igem.org/Team:San_Andres/Description"
+
style="color: rgb(0, 0, 0);"> Project </a>
+
      </td>
+
      <td style="border: 2px solid black;"
+
onmouseover="this.bgColor='#ffba00'"
+
onmouseout="this.bgColor='#fcff00'" align="center"
+
bgcolor="#fcff00" height="45">
+
      <a href="https://2015.igem.org/Team:San_Andres/Notebook"
+
style="color: rgb(0, 0, 0);"> Statistics</a>
+
      </td>
+
      <td style="border: 2px solid black;"
+
onmouseover="this.bgColor='#ffba00'"
+
onmouseout="this.bgColor='#fcff00'" align="center"
+
bgcolor="#fcff00" height="45">
+
      <a href="https://2015.igem.org/Team:San_Andres/Experiments"
+
style="color: rgb(0, 0, 0);"> Enzymes</a>
+
      </td>
+
      <td style="border: 2px solid black;"
+
onmouseover="this.bgColor='#ffba00'"
+
onmouseout="this.bgColor='#fcff00'" align="center"
+
bgcolor="#fcff00" height="45">
+
      <a href="https://2015.igem.org/Team:San_Andres/Parts"
+
style="color: rgb(0, 0, 0);"> Parts</a>
+
      </td>
+
      <td style="border: 2px solid black;"
+
onmouseover="this.bgColor='#ffba00'"
+
onmouseout="this.bgColor='#fcff00'" align="center"
+
bgcolor="#fcff00" height="45">
+
      <a href="https://2015.igem.org/Team:San_Andres/Modeling"
+
style="color: rgb(0, 0, 0);"> Metodology</a>
+
      </td>
+
      <td style="border: 2px solid black;"
+
onmouseover="this.bgColor='#ffba00'"
+
onmouseout="this.bgColor='#fcff00'" align="center"
+
bgcolor="#fcff00" height="45">
+
      <a href="https://2015.igem.org/Team:San_Andres/Results"
+
style="color: rgb(0, 0, 0);"> Results </a>
+
      </td>
+
      <td style="border: 2px solid black;"
+
onmouseover="this.bgColor='#ffba00'"
+
onmouseout="this.bgColor='#fcff00'" align="center"
+
bgcolor="#fcff00" height="45">
+
      <a href="https://2015.igem.org/Team:San_Andres/Collaborations"
+
style="color: rgb(0, 0, 0);"> Future Projections </a>
+
      </td>
+
      <td align="center">
+
      <a href="https://igem.org/Team_List?year=2015"><img
+
src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"
+
width="55"></a></td>
+
    </tr>
+
  </tbody>
+
</table>
+
<div class="gwd-div-2ed9"></div>
+
<div class="gwd-div-7xzo gwd-a-1thu">
+
<h1>Enzymes</h1>
+
<big>During our investigation we sought the perfect enzyme to
+
degrade the gluten, and we found:<br>
+
</big>
+
<ul>
+
  <li><big>Prolyl Endopeptidase: It is a kind of serine
+
protease capable of breaking peptide bonds following to the group of
+
terminal carboxyl of a PROLINE residue. While he was a candidate for a
+
possible treatment failed to meet expectations, because its activity is
+
a high pH, which is not suitable for an average digestive. Another
+
reason was that it degrades slowly, which would have resulted in a
+
longer and inefficient treatment.</big></li>
+
</ul>
+
<div style="text-align: center;"><img
+
alt="File:Prolil.jpg"
+
src="https://static.igem.org/mediawiki/2015/c/c0/Prolil.jpg"
+
height="376" width="565"><br>
+
<br>
+
<div style="text-align: left;">
+
<ul>
+
  <li><big>Kumamolisin As: It is the first known example of
+
a collagenase derived from the family of the sedolisin. This operates
+
at high temperatures and low pH levels. Its characteristics, together
+
with those predicted are measured by comparison between a collagenase
+
and a peptidase from serine, which are related to the enzyme
+
preference, to thus Digest collagen as gluten.</big></li>
+
</ul>
+
<div style="text-align: center;"><img
+
alt="File:2-2-2 2.jpg"
+
src="https://static.igem.org/mediawiki/2015/b/be/2-2-2_2.jpg"
+
height="243" width="470"><br>
+
<ul style="text-align: left;">
+
  <li><big>KumaMax (G319S, D358G, D368H, N281D): It is a
+
mutation of the Kumamolisin As, which is designed to digest way more
+
efficient gluten, because that can work at pH levels much more lower
+
than the original enzyme (a pH of 4.0) which is excellent for the
+
average digestive system. <span class="hps">It was
+
created by the team IGEM Washington 2011</span>. Other advantages
+
are:</big></li>
+
</ul>
+
<ol style="text-align: left;">
+
  <li><big>It is resistant to high temperatures and acidity
+
of the stomach.</big></li>
+
  <li><big>It is heat stable, in others words, it is
+
resistant to all changes in their physical and chemical structure.</big></li>
+
  <li><big>It is easily repairable and creable.</big></li>
+
</ol>
+
<div style="text-align: center;"><img
+
alt="File:175px-Washington Bottle.jpg"
+
src="https://static.igem.org/mediawiki/2015/d/d0/175px-Washington_Bottle.jpg"
+
height="263" width="175"> <img
+
alt="File:250px-Washington Kumamolisin VS SC-PEP.png"
+
src="https://static.igem.org/mediawiki/2015/1/1b/250px-Washington_Kumamolisin_VS_SC-PEP.png"
+
height="213" width="250">&nbsp;<img
+
alt="File:250px-Washington Kuma Bonded triad.png"
+
src="https://static.igem.org/mediawiki/2015/6/62/250px-Washington_Kuma_Bonded_triad.png"
+
height="193" width="250"></div>
+
<br>
+
<div style="text-align: left;"></div>
+
</div>
+
</div>
+
</div>
+
<div style="text-align: left;"><br>
+
<div style="text-align: center;"><br>
+
<div style="text-align: left;"><big><br>
+
</big>
+
<div style="text-align: center;">
+
<div style="text-align: center;"><span id="result_box"
+
class="" lang="en"><span class="hps"></span><span
+
class="hps"></span></span><br>
+
</div>
+
<span id="result_box" class="" lang="en"><span
+
class="hps"></span></span></div>
+
<span id="result_box" class="" lang="en"><span
+
class="hps"><br>
+
</span></span></div>
+
</div>
+
</div>
+
</div>
+
<div style="text-align: center;"><br>
+
</div>
+
<div class="gwd-div-a9c8"></div>
+
<br>
+
<br>
+
</body>
+
</html>
+

Latest revision as of 02:02, 23 July 2015