|
|
| (7 intermediate revisions by the same user not shown) |
| Line 1: |
Line 1: |
| − | <html>
| |
| | | | |
| − | <head data-gwd-animation-mode="quickMode">
| |
| − | <title>wiki</title>
| |
| − | <meta http-equiv="Content-Type" content="text/html; charset=utf-8">
| |
| − | <meta name="generator" content="Google Web Designer 1.3.2.0521">
| |
| − | <style type="text/css">
| |
| − | html, body {
| |
| − | width: 100%;
| |
| − | height: 100%;
| |
| − | margin: 0px;
| |
| − | }
| |
| − | body {
| |
| − | -webkit-transform: perspective(1400px) matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1);
| |
| − | -webkit-transform-style: preserve-3d;
| |
| − | background-color: transparent;
| |
| − | }
| |
| − | </style>
| |
| − | </head>
| |
| − |
| |
| − | <body>
| |
| − | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
| |
| − | <title></title>
| |
| − | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
| |
| − | <title></title>
| |
| − | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
| |
| − | <title></title>
| |
| − | <img style="width: 959px; height: 359px;" src="https://static.igem.org/mediawiki/2015/0/09/Firma_800.jpg" gwd-img-541d="" class="gwd-img-voqe">
| |
| − | <title>wiki 2</title>
| |
| − | <meta http-equiv="Content-Type" content="text/html; charset=utf-8">
| |
| − | <meta name="generator" content="Google Web Designer 1.3.2.0521">
| |
| − | <style type="text/css" class="">
| |
| − | html, body {
| |
| − | width: 100%;
| |
| − | height: 10%;
| |
| − | margin: 0px;
| |
| − | }
| |
| − | body {
| |
| − | -webkit-transform: perspective(1400px) matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1);
| |
| − | -webkit-transform-style: preserve-3d;
| |
| − | background-color: transparent;
| |
| − | }
| |
| − | .gwd-table-nb4m {
| |
| − | font-size: 24px;
| |
| − | -webkit-transform: translate3d(0px, -2px, 0px);
| |
| − | -webkit-transform-style: preserve-3d;
| |
| − | background-image: none;
| |
| − | background-color: rgb(255, 255, 255);
| |
| − | }
| |
| − | .gwd-td-b6db {
| |
| − | border: 1px solid black;
| |
| − | }
| |
| − | .gwd-a-g1jl {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-o5pu {
| |
| − | border: 1px solid black;
| |
| − | }
| |
| − | .gwd-a-7xj9 {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-jbkf {
| |
| − | border: 1px solid black;
| |
| − | }
| |
| − | .gwd-td-f88u {
| |
| − | border: 1px solid black;
| |
| − | }
| |
| − | .gwd-a-8khq {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-j5rf {
| |
| − | border: 1px solid black;
| |
| − | }
| |
| − | .gwd-a-50lt {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-3tnb {
| |
| − | border: 1px solid black;
| |
| − | }
| |
| − | .gwd-a-oa0r {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-ki1q {
| |
| − | border: 1px solid black;
| |
| − | }
| |
| − | .gwd-a-3fxh {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-0vlu {
| |
| − | border: 1px solid black;
| |
| − | }
| |
| − | .gwd-a-mwsi {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-zyck {
| |
| − | border: 1px solid black;
| |
| − | }
| |
| − | .gwd-a-j8kh {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-uz3t {
| |
| − | border: 1px solid black;
| |
| − | }
| |
| − | .gwd-a-83hm {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-fnbn {
| |
| − | border: 1px solid black;
| |
| − | }
| |
| − | .gwd-a-80zp {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-asn4 {
| |
| − | border: 1px solid black;
| |
| − | }
| |
| − | .gwd-a-chbv {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-8c55 {
| |
| − | border: 1px solid black;
| |
| − | }
| |
| − | .gwd-a-rf1i {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-jce5 {
| |
| − | border: 1px solid black;
| |
| − | }
| |
| − | .gwd-a-bm82 {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-7vtt {
| |
| − | border: 1px solid black;
| |
| − | }
| |
| − | .gwd-a-ffa8 {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-aqks {
| |
| − | border: 1px solid black;
| |
| − | }
| |
| − | .gwd-a-1thu {
| |
| − | color: rgb(0, 0, 0);
| |
| − | background-image: none;
| |
| − | background-color: rgb(255, 255, 255);
| |
| − | }
| |
| − | .gwd-td-dgpg {
| |
| − | border: 1px solid black;
| |
| − | }
| |
| − | .gwd-a-cpqy {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-788u {
| |
| − | border: 1px solid black;
| |
| − | }
| |
| − | .gwd-a-z37b {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-div-2ed9 {
| |
| − | position: absolute;
| |
| − | width: 50px;
| |
| − | height: 31px;
| |
| − | left: 713px;
| |
| − | top: 92px;
| |
| − | font-family: 'Times New Roman';
| |
| − | text-align: left;
| |
| − | color: rgb(0, 0, 0);
| |
| − | background-image: none;
| |
| − | }
| |
| − | .gwd-td-1it6 {
| |
| − | border: 2px solid black;
| |
| − | }
| |
| − | .gwd-a-cfz2 {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-gm83 {
| |
| − | border: 2px solid black;
| |
| − | }
| |
| − | .gwd-a-d494 {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-werd {
| |
| − | border: 2px solid black;
| |
| − | }
| |
| − | .gwd-a-feiv {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-pvfp {
| |
| − | border: 2px solid black;
| |
| − | }
| |
| − | .gwd-a-laum {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-yx5y {
| |
| − | border: 2px solid black;
| |
| − | }
| |
| − | .gwd-a-iekk {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-844w {
| |
| − | border: 2px solid black;
| |
| − | }
| |
| − | .gwd-a-njb2 {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-g48r {
| |
| − | border: 2px solid black;
| |
| − | }
| |
| − | .gwd-a-r862 {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-sdu6 {
| |
| − | border: 2px solid black;
| |
| − | }
| |
| − | .gwd-a-tjrm {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-td-4dr4 {
| |
| − | border: 2px solid black;
| |
| − | }
| |
| − | .gwd-a-yt4x {
| |
| − | color: rgb(0, 0, 0);
| |
| − | }
| |
| − | .gwd-div-oiqr {
| |
| − | background-image: none;
| |
| − | background-color: rgb(255, 255, 255);
| |
| − | }
| |
| − | .gwd-img-voqe {
| |
| − | background-image: none;
| |
| − | }
| |
| − | </style>
| |
| − | <!--navigation menu -->
| |
| − | <table class="gwd-table-nb4m editable" width="100%">
| |
| − | <tbody>
| |
| − | <tr heigth="15px">
| |
| − | </tr>
| |
| − | <tr heigth="75px">
| |
| − | <td style="border: 2px solid black;" align="center" bgcolor="FF8600" height="45" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#FFBA00'">
| |
| − | <a href="https://2015.igem.org/Team:San_Andres" style="color: rgb(0, 0, 0);">Home </a>
| |
| − | </td>
| |
| − | <td style="border: 2px solid black;" align="center" bgcolor="#FF8600" height="45" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
| |
| − | <a href="https://2015.igem.org/Team:San_Andres/Team" style="color: rgb(0, 0, 0);"> Team </a>
| |
| − | </td>
| |
| − | <td style="border: 2px solid black;" align="center" bgcolor="#FF8600" height="45" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
| |
| − | <a href="https://2015.igem.org/Team:San_Andres/Description" style="color: rgb(0, 0, 0);"> Project </a>
| |
| − | </td>
| |
| − | <td style="border: 2px solid black;" align="center" bgcolor="#FF8600" height="45" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
| |
| − | <a href="https://2015.igem.org/Team:San_Andres/Notebook" style="color: rgb(0, 0, 0);"> Statistics</a>
| |
| − | </td>
| |
| − | <td style="border: 2px solid black;" align="center" bgcolor="#FF8600" height="45" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
| |
| − | <a href="https://2015.igem.org/Team:San_Andres/Experiments" style="color: rgb(0, 0, 0);"> Enzymes</a>
| |
| − | </td>
| |
| − | <td style="border: 2px solid black;" align="center" bgcolor="#FF8600" height="45" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
| |
| − | <a href="https://2015.igem.org/Team:San_Andres/Parts" style="color: rgb(0, 0, 0);"> Parts</a>
| |
| − | </td>
| |
| − | <td style="border: 2px solid black;" align="center" bgcolor="#FF8600" height="45" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
| |
| − | <a href="https://2015.igem.org/Team:San_Andres/Modeling" style="color: rgb(0, 0, 0);"> Metodology</a>
| |
| − | </td>
| |
| − | <td style="border: 2px solid black;" align="center" bgcolor="#FF8600" height="45" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
| |
| − | <a href="https://2015.igem.org/Team:San_Andres/Results" style="color: rgb(0, 0, 0);"> Results </a>
| |
| − | </td>
| |
| − | <td style="border: 2px solid black;" align="center" bgcolor="#FF8600" height="45" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
| |
| − | <a href="https://2015.igem.org/Team:San_Andres/Collaborations" style="color: rgb(0, 0, 0);"> Future Projections </a>
| |
| − | </td>
| |
| − | <td align="center">
| |
| − | <a href="https://igem.org/Team_List?year=2015">
| |
| − | <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55">
| |
| − | </a>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody>
| |
| − | </table>
| |
| − | <div class="gwd-div-2ed9"></div>
| |
| − | <div class="gwd-div-7xzo gwd-a-1thu">
| |
| − | <h1>Enzymes</h1>
| |
| − | <big>During our investigation we sought the perfect enzyme to
| |
| − | degrade the gluten, and we found:<br>
| |
| − | </big>
| |
| − | <ul>
| |
| − | <li><big>Prolyl Endopeptidase: It is a kind of serine
| |
| − | protease capable of breaking peptide bonds following to the group of
| |
| − | terminal carboxyl of a PROLINE residue. While he was a candidate for a
| |
| − | possible treatment failed to meet expectations, because its activity is
| |
| − | a high pH, which is not suitable for an average digestive. Another
| |
| − | reason was that it degrades slowly, which would have resulted in a
| |
| − | longer and inefficient treatment.</big></li>
| |
| − | </ul>
| |
| − | <div style="text-align: center;"><img
| |
| − | alt="File:Prolil.jpg"
| |
| − | src="https://static.igem.org/mediawiki/2015/c/c0/Prolil.jpg"
| |
| − | height="376" width="565"><br>
| |
| − | <br>
| |
| − | <div style="text-align: left;">
| |
| − | <ul>
| |
| − | <li><big>Kumamolisin As: It is the first known example of
| |
| − | a collagenase derived from the family of the sedolisin. This operates
| |
| − | at high temperatures and low pH levels. Its characteristics, together
| |
| − | with those predicted are measured by comparison between a collagenase
| |
| − | and a peptidase from serine, which are related to the enzyme
| |
| − | preference, to thus Digest collagen as gluten.</big></li>
| |
| − | </ul>
| |
| − | <div style="text-align: center;"><img
| |
| − | alt="File:2-2-2 2.jpg"
| |
| − | src="https://static.igem.org/mediawiki/2015/b/be/2-2-2_2.jpg"
| |
| − | height="243" width="470"><br>
| |
| − | <ul style="text-align: left;">
| |
| − | <li><big>KumaMax (G319S, D358G, D368H, N281D): It is a
| |
| − | mutation of the Kumamolisin As, which is designed to digest way more
| |
| − | efficient gluten, because that can work at pH levels much more lower
| |
| − | than the original enzyme (a pH of 4.0) which is excellent for the
| |
| − | average digestive system. <span class="hps">It was
| |
| − | created by the team IGEM Washington 2011</span>. Other advantages
| |
| − | are:</big></li>
| |
| − | </ul>
| |
| − | <ol style="text-align: left;">
| |
| − | <li><big>It is resistant to high temperatures and acidity
| |
| − | of the stomach.</big></li>
| |
| − | <li><big>It is heat stable, in others words, it is
| |
| − | resistant to all changes in their physical and chemical structure.</big></li>
| |
| − | <li><big>It is easily repairable and creable.</big></li>
| |
| − | </ol>
| |
| − | <div style="text-align: center;"><img
| |
| − | alt="File:175px-Washington Bottle.jpg"
| |
| − | src="https://static.igem.org/mediawiki/2015/d/d0/175px-Washington_Bottle.jpg"
| |
| − | height="263" width="175"> <img
| |
| − | alt="File:250px-Washington Kumamolisin VS SC-PEP.png"
| |
| − | src="https://static.igem.org/mediawiki/2015/1/1b/250px-Washington_Kumamolisin_VS_SC-PEP.png"
| |
| − | height="213" width="250"> <img
| |
| − | alt="File:250px-Washington Kuma Bonded triad.png"
| |
| − | src="https://static.igem.org/mediawiki/2015/6/62/250px-Washington_Kuma_Bonded_triad.png"
| |
| − | height="193" width="250"></div>
| |
| − | <br>
| |
| − | <div style="text-align: left;"></div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div style="text-align: left;"><br>
| |
| − | <div style="text-align: center;"><br>
| |
| − | <div style="text-align: left;"><big><br>
| |
| − | </big>
| |
| − | <div style="text-align: center;">
| |
| − | <div style="text-align: center;"><span id="result_box"
| |
| − | class="" lang="en"><span class="hps"></span><span
| |
| − | class="hps"></span></span><br>
| |
| − | </div>
| |
| − | <span id="result_box" class="" lang="en"><span
| |
| − | class="hps"></span></span></div>
| |
| − | <span id="result_box" class="" lang="en"><span
| |
| − | class="hps"><br>
| |
| − | </span></span></div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div style="text-align: center;"><br>
| |
| − | </div>
| |
| − | <div class="gwd-div-a9c8"></div>
| |
| − | <br>
| |
| − | <br>
| |
| − | </body>
| |
| − | </html>
| |