Team:Santa Clara/The Problem
The Problem
Bioreactor technology allows us, as synthetic biologists, to take our technology to the large scale. It denotes cell culture methods that allow for the mass production of organisms and their products by facilitating growth in various apparatuses. The goal of these systems is to create an environment for elevated cell growth to promote the production and/or degradation of various substances. These systems have been optimized to fit endless applications but one problem remains unresolved. In these cultures, cells are designed to reach high cell densities, which leads to an increased build up of metabolic acid. The increasing presence of acid in the environment adversely affects cell processes because weak acids can easily diffuse back through the cell membrane to inhibit and degrade DNA, as well as degrade proteins essential to cell survival. To offset this issue and maintain cell vitality, the culture must be supplemented with various basic solutions to neutralize the acid. This increases the running cost of the system, through the cost of the solutions themselves, as well as the production and implementation of systems to distribute the base. It also results in slowed cell growth due to the exposure of highly concentrated base.
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