Difference between revisions of "Team:TU Darmstadt/Notebook/sec1/K1602043"
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| − | K1602040 was digested with XbaI and PstI and ligated into T7-pSB1A2. Competent E. coli Top 10 were transformed via heat shock. The plasmids were extracted and digested with EcoRI and PstI and cloned into pSB1C3. Competent E. coli Top 10 were transformed and colonies were screened by colony PCR. Plasmids were extracted and used for protein expression in E. coli strain BL21. | + | <a href=" https://2015.igem.org/Team:TU_Darmstadt/Notebook/sec1/K1602040 " title="Opens internal link in current window" class="internal link">K1602040</a> was digested with XbaI and PstI and ligated into T7-pSB1A2. Competent E. coli Top 10 were transformed via heat shock. The plasmids were extracted and digested with EcoRI and PstI and cloned into pSB1C3. Competent E. coli Top 10 were transformed and colonies were screened by colony PCR. Plasmids were extracted and used for protein expression in E. coli strain BL21. |
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Revision as of 20:08, 16 September 2015
K1602043 - T7-B0034-enhanced yellow fluorescent protein-GBD-ligand (T7-B0034-eYFP-GBDlig)
K1602040 was digested with XbaI and PstI and ligated into T7-pSB1A2. Competent E. coli Top 10 were transformed via heat shock. The plasmids were extracted and digested with EcoRI and PstI and cloned into pSB1C3. Competent E. coli Top 10 were transformed and colonies were screened by colony PCR. Plasmids were extracted and used for protein expression in E. coli strain BL21.