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| − | <h3> K1602045 - T7-B0034-enhanced yellow fluorescent protein-PDZ-ligand (T7-B0034-eYFP-PDZlig)</h3>
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| − | <a href=" https://2015.igem.org/Team:TU_Darmstadt/Notebook/sec1/K1602042 " title="Opens internal link in current window" class="internal link">K1602042</a> was digested with <em>Xba</em>I and <em>Pst</em>I and ligated into T7-pSB1A2. Competent <em>E. coli</em> Top 10 were transformed via heat shock. The plasmids were extracted and digested with <em>EcoR</em>I and <em>Pst</em>I and cloned into pSB1C3. Competent <em>E. coli</em> Top 10 were transformed and colonies were screened by colony PCR. Plasmids were extracted and used for protein expression in <em>E. coli</em> strain BL21.
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| − | </div></html>
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