Difference between revisions of "Team:Tokyo-NoKoGen"

 
(41 intermediate revisions by 2 users not shown)
Line 1: Line 1:
 
{{TeamTokyoNokogen}}
 
{{TeamTokyoNokogen}}
−
<!DOCTYPE html>
 
−
<html xmlns="http://www.w3.org/1999/xhtml">
 
−
<head>
 
  
−
<STYLE TYPE="text/css"><!--
+
<html lang="ja">
−
DIV.tuzukiwaku{border:1px solid #badefe;padding:3px;margin:3px;display:none;
+
−
}-->
+
−
</STYLE>
+
−
+
−
+
−
<SCRIPT language="JavaScript"><!--
+
−
function hiraku(geID){
+
−
document.getElementById(geID).style.display = "block";
+
−
}
+
−
function tatamu(geID){
+
−
document.getElementById(geID).style.display = "none";
+
−
}
+
−
//-->
+
−
</SCRIPT>
+
−
 
+
−
<NOSCRIPT>
+
−
 
+
−
<STYLE TYPE="text/css"><!--
+
−
DIV.tuzukiwaku{display:block;color:#ffffff;}
+
−
--></STYLE>
+
−
</NOSCRIPT>
+
−
<meta http-equiv="Content-Type" content="text/html; charset=utf-8"/>
+
−
 
+
−
    <title>折りたたみ</title>
+
−
</head>
+
−
<body>
+
−
<dl>
+
−
<dt><b>Metabolic pathway in the termite</b></dt>
+
−
<dd>
+
−
Most of the insects store their energy source in the form of the disaccharide trehalose in their blood.<br>
+
−
They utilize trehalose in their cell membrane by reconverting it into glucose by the enzyme, trehalase (α-glucoside-1-glucohydrolase), which hydrolyze trehalose. [1]<br>
+
−
Additionally, termites has another metabolic pathway. Termites feed on woods, papers and whatever containing cellulose. They can digest cellulose into glucose in their gut using the characteristic enzyme, cellulase.<br>
+
−
ExTermite Coli targets both of the pathway in the termite to cut their energy resource and exterminate termites.<br>
+
−
</dd>
+
−
</dl>
+
−
<A HREF="JavaScript:hiraku('tuzuki1');">< Details：Higher termite and Lower termite ></A><BR>
+
−
<DIV CLASS="tuzukiwaku" ID="tuzuki1">
+
−
<dl>
+
−
<dt><b>Higher termite and Lower termite</b></dt>
+
−
<dd>
+
−
  Glucose, end products of cellulose digestion, is used as the energy sources by termites. However, the method of digestion varies depending on the species of termites. <br>
+
−
　Termites can be classified as higher termites and lower termites. In higher termites, cellulose digestion appears to be mediated solely by cellulase secreted by the termites. On the other hand, lower termites digest cellulose by cellulase secreted both by termites and by the protozoa. [2]<br>
+
−
  As for our keeping termites, Coptotermes formosanus and Reticulitermes speratus are categorized as lower termites. ExTermite Coli targets the inhibition of the cellulose activity no matter where the enzyme secreted. In other words, ExTermite Coli can affect both of the higher termites and the lower termites.<br>
+
−
</dd>
+
−
</dl>
+
−
<A href="JavaScript:tatamu('tuzuki1');">たたむ</A>
+
−
</DIV>
+
−
<dl>
+
−
<dt><b>Inhibition of two enzymes activity by one enzyme</b></dt>
+
−
<dd>
+
−
From the above reasons, we have to inhibit the activity of trehalase and cellulose. To achieve the purpose, we planed to use the enzyme, glucoside-3-dehydrogenase ( G3DH ). G3DH  is an enzyme which oxidize third hydroxyl group of pyranose. Since this enzyme has wide substrate specificity, it can catalyze the oxidization of many monosaccharides (like glucose and methyl-α-D-glucoside) and disaccharides (like trehalose and cellobiose).<br>
+
−
We focused on G3DH as the enzyme which can produce two materials to inhibit the metabolic pathway of the termite. One is inhibitor of trehalase from trehalose from the specific characteristic of G3DH. The other is inhibitor of cellulase from cellobiose which is one possibility that G3DH can produce.
+
−
　　According to our hypothesis, we did our experiments using G3DH. <br>
+
−
</dd>
+
−
</dl>
+
−
<A HREF="JavaScript:hiraku('tuzuki2');">< Details：Our hypothesis about products of G3DH></A><BR>
+
−
<DIV CLASS="tuzukiwaku" ID="tuzuki2">
+
−
<dl>
+
−
<dt><b>Our hypothesis about products of G3DH</b></dt>
+
−
<dd>
+
−
  3,3’-diketotorehalose (3,3’dkT) is a trehalose derivative based on enzymatic oxidation of trehalose by G3DH. Both third hydroxyl group of glucopyranosides of 3,3’-dkT were oxidized. 3,3’dkT was revealed to show an inhibitory effect toward pig kidney and Bombyx mori trehalase. [6] Therefore, 3,3’dkT probably show an inhibit effect of 3,3’dkT toward termite trehalase. Moreover, Cellobiose derivative based on enzymatic oxidation by G3DH maybe show inhibit effect toward cellulase.
+
−
  First of all, we purified G3DH from cultivated host strain.
+
−
</dd>
+
−
</dl>
+
−
<A href="JavaScript:tatamu('tuzuki2');">たたむ</A>
+
−
</DIV>
+
−
 
+
−
</body>
+
−
</html>
+
−
 
+
−
 
+
−
 
+
−
 
+
−
<html>
+
 
<style>
 
<style>
 
html, body{
 
html, body{
Line 102: Line 25:
 
position:absolute;
 
position:absolute;
  
−
         top:0px;
+
         top:-10px;
  
 
}
 
}
 +
 
div#box1 /*1段目の箱*/ {
 
div#box1 /*1段目の箱*/ {
 
width:900px;
 
width:900px;
Line 117: Line 41:
 
width:600px;
 
width:600px;
 
height:300px;
 
height:300px;
−
margin-top:10px;
+
margin-top:4px;
 
margin-left:10px;
 
margin-left:10px;
 
background:#FFFFCC;
 
background:#FFFFCC;
−
     
 
 
display: inline-block; _display: inline;
 
display: inline-block; _display: inline;
 
float:left;
 
float:left;
 
cursor:pointer;
 
cursor:pointer;
−
}
+
}
 +
 +
.overview2{
 +
　width:500px;
 +
　height:270px;
 +
　　　 margin-top:10px;
 +
　margin-left:15px;
 +
　background:#ffffff;
 +
display: inline-block; _display: inline;
 +
float:left;
 +
cursor :pointer;
 +
z-index:3;
 +
}
  
 
.team {
 
.team {
 
width:260px;
 
width:260px;
−
height:135px;
+
height:115px;
 
       position:relative;
 
       position:relative;
 
left:15px;
 
left:15px;
Line 143: Line 78:
 
         .notebook  {
 
         .notebook  {
 
width:260px;
 
width:260px;
−
height:140px;
+
height:130px;
 
position:relative;
 
position:relative;
 
margin-top:5px;
 
margin-top:5px;
Line 167: Line 102:
 
       left:0px;
 
       left:0px;
 
}
 
}
−
 
+
.white{
 +
height:135px;
 +
background:#ffffff;
 +
}
 +
   
 +
 
 
.facebook {
 
.facebook {
 
width:200px;
 
width:200px;
Line 196: Line 136:
 
cursor:pointer;
 
cursor:pointer;
 
     　 　}
 
     　 　}
 +
   
  
 
.project {
 
.project {
 
width:650px;
 
width:650px;
−
height:300px;
+
height:260px;
−
margin-top:-300px;
+
margin-top:-290px;
−
margin-right:10px;
+
margin-right:0px;
 
background: #79d480;
 
background: #79d480;
 
float:right;
 
float:right;
Line 262: Line 203:
 
     line-height:2;  
 
     line-height:2;  
 
   }
 
   }
−
h2 {
+
h2{
−
     font-size:20px;
+
     font-size:25px;
 +
    font-family:fantasy;
 +
    text-decoration:underline;
 +
    }
 +
h1{
 +
    font-size:14px;
 +
    font-family:fantasy;
 +
    text-decoration:none;
 +
    line-height: 120%;
 +
    }
 +
 
 +
h3{
 +
    font-size:14px;
 
     font-family:fantasy;
 
     font-family:fantasy;
 
     text-decoration:none;
 
     text-decoration:none;
−
  }
+
    line-height: 120%;
 +
    }
  
 
div#main {
 
div#main {
Line 318: Line 272:
 
     width:30%;
 
     width:30%;
 
     padding:5%;
 
     padding:5%;
−
     border:solid 1px #CCC;
+
     border:solid 0px #CCC;
 
     margin:0px auto 20px;
 
     margin:0px auto 20px;
 
}
 
}
Line 331: Line 285:
 
}
 
}
 
</style>
 
</style>
−
 
 
<div id="wrap">
 
<div id="wrap">
  
Line 340: Line 293:
  
 
　　<div class="overview">
 
　　<div class="overview">
−
　　 <h2>Introduction</h2>
+
  　　　　　　　 <div class="overview2">
−
  　　　　　<!--　　  <img src="http://junk-blog.com/wp-content/uploads/2014/09/sample_image.jpg" alt="サンプル画像" width="550" height="250"alt="Overview"/> -->
+
<div style="padding: 5px; border: none; background-color: #ffffff; margin: 10px; "> <h2>Introduction</h2>
−
  　　
+
<img src="https://static.igem.org/mediawiki/2015/e/e6/NK-image1.jpeg" align=right width=150px; height=120px;>
−
  　　　　　　　  <a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Overview" target="_blank" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7 ></a>
+
<h3>What kind  image do you have when you hear the word “termite”??<br><br>
−
                         
+
Actually, this tiny insect has unimaginable voracity. <br>
−
   　　　　　　　  
+
They cause great damage for human life, but there is no way to exterminate terites completely and safely. <br><br>
−
　　　　</div>
+
To solve this problem, <br>Tokyo-NoKoGen “ExTermite Coli”, a new pesticides for Termites !!<br>
 +
</h3>
 +
 +
</div>  　　　　　　　   
 +
<a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Overview" target="_blank" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor='#e7e7e7' ></a>
 +
   　　　　　　　 </div>
 +
　　　　 </div>
 +
　　　　
 
　　　　
 
　　　　
 
　　<div class="team">
 
　　<div class="team">
−
　  <h2>Team</h2>
+
<div style="padding: 5px; border: none; background-color: #ffffff; margin: 10px;"> <h2>Team</h2>
−
　<a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Team" target="_blank" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7 ></a>
+
<img src="https://static.igem.org/mediawiki/2015/0/0f/NK-image23.jpeg"  width=50px; height=50px;>
−
       　　 </div>  
+
</div>
 +
　 <a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Team" target="_blank" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7 ></a>
 +
       　　 </div>  
 
       　　  
 
       　　  
 
               <div class="notebook">
 
               <div class="notebook">
−
　<h2>Notebook</h2>
+
<div style="padding: 5px; border: none; background-color: #ffffff; margin: 10px;">
 +
  <h2>Protocol</h2>
 +
<img src="https://static.igem.org/mediawiki/2015/5/5e/NK-image11.jpeg" width=50px; height=50px;>
 +
<br>
 +
</div>
 
　<a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Notebook" target="_blank" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7 ></a>
 
　<a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Notebook" target="_blank" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7 ></a>
 
       　　  </div>
 
       　　  </div>
Line 362: Line 328:
 
<div id="box2"/*見えない2段目のボックス*/>　
 
<div id="box2"/*見えない2段目のボックス*/>　
 
<div class="facebook">
 
<div class="facebook">
−
   　　　　　　　 <h2>facebook</h2>
+
   　　　　<p style="position: relative;">
−
   　　　　　　　<a href="https://www.facebook.com/tokyo.nokogen" target="_blank"></a>
+
   　　　　　　<a href="https://www.facebook.com/tokyo.nokogen" target="_blank">
−
   　　　　　　　
+
  　　　　　　 <img src="https://static.igem.org/mediawiki/2015/c/cc/NK-image17.jpeg" style ="position:absolute; top:0px;left:20px;" width="150" height="100"><br/>
 +
  　　　　　　<span style="position: absolute; top: 0px; left: 5px; width: 10 px;">
 +
  　　　　　   </span>
 +
  　　　　　　</a>
 +
              </p>
 
   　　</div>
 
   　　</div>
−
  　　<div class="twitter">
+
　　<div class="twitter">
−
   　　　　　　　 <h2>twitter</h>
+
<div style="border: none; background-color: #ffffff; margin: 10px;">    
−
  　　　　　　　 <a href="https://twitter.com/iGEM_Nokogen" target="_blank"></a>
+
　　  　　　　　<h2>Twitter</h2>
−
  　　　　　　　
+
  　　　　　 <p style="position: relative;">
 +
                      <a href="https://twitter.com/iGEM_Nokogen" target="_blank">
 +
                      <img src="https://static.igem.org/mediawiki/2015/8/80/NKtwitter.jpeg" style ="position:absolute;top:-70px;left:20px;" width="135" height="90">
 +
                      </a>
 +
                    </p>
 +
                </div>
 
   　　</div>
 
   　　</div>
−
   　　
+
   　　  　　
 
  　　 　<div class="project">
 
  　　 　<div class="project">
−
  　　　　　　<h2>project</h2>　
+
　　 　 <div style="padding: 0px; border: none; background-color: #ffffff; margin: 4px;">
−
  　　　　　　<a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Description" target="_blank"></a> 　　　　　　
+
<h2>project</h2>
 +
  <img src="https://static.igem.org/mediawiki/2015/2/23/NK-image7.jpeg" align=right width=130px; height=80px;>
 +
 
 +
<h3>The exterminateon of termites by ExTermite Coli involves three steps.<br>
 +
We use “pseudo egg” to distribute the engineered E.coli.  Using this unique tool, the engineered E.coli carried into the termite’s body. <br>
 +
In the termite’s body, it  produces toxic material to inhibit the metabolic pathway of the termites and exterminate termites.
 +
After extermination of the termites, ExTermite Coli loses its function by light-induced lysis. <br>
 +
  On the project page, we explain about the details of our project, ExTermite Coli.</h3>
 +
 
 +
                          <span style="position: absolute; top: 5px; left: 5px; width: 10px;"></span>　　　　　　  
 +
  　　　</div>
 +
  　　　<a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Description" target="_blank"></a>  
 +
 
 
   　　　</div>
 
   　　　</div>
 
      
 
      
 
       <div id="box3"/*見えない3段目のボックス*/>　　
 
       <div id="box3"/*見えない3段目のボックス*/>　　
 
   　　<div class="Ache">
 
   　　<div class="Ache">
−
   　　　　　　　 <h2>Achievement</h2>
+
  　　　　　　　<div style="padding: 5px; border: none; background-color: #ffffff; margin: 10px;">
 +
   　　　　　　　<h2>Achievement</h2>
 +
  　　　　　　　  　　　　　　　  　　</div>
 
   　　　　　　　 <a href="https://2015.igem.org/Team:Tokyo-NoKoGen/achievement" target="_blank"></a>  
 
   　　　　　　　 <a href="https://2015.igem.org/Team:Tokyo-NoKoGen/achievement" target="_blank"></a>  
−
  　　</div>
+
</div>
 +
  　　
 +
  　　
 
   　　<div class="Human">
 
   　　<div class="Human">
 +
  　　　　　　　 <div style="padding: 5px; border: none; background-color: #ffffff; margin: 10px;">
 
   　　　　　　　 <h2>Human practice</h2>
 
   　　　　　　　 <h2>Human practice</h2>
 +
  　　　　　　　 　</div>
 
   　　　　　　　  <a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Practices" target="_blank"></a>
 
   　　　　　　　  <a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Practices" target="_blank"></a>
 
   　　</div>
 
   　　</div>
 +
  　　
 
   　　<div class="parts">
 
   　　<div class="parts">
 +
  　　　　　　　 <div style="padding: 5px; border: none; background-color: #ffffff; margin: 10px;">
 
   　　　　　　　 <h2>Parts</h2>
 
   　　　　　　　 <h2>Parts</h2>
 +
</div>
 
   　　　　　　　  <a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Parts" target="_blank"></a>
 
   　　　　　　　  <a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Parts" target="_blank"></a>
 
   　　</div>
 
   　　</div>
 +
  　　
 +
  　　
 
   　　 <div class="safety">
 
   　　 <div class="safety">
 +
  　　　　　　　 <div style="padding: 5px; border: none; background-color: #ffffff; margin: 10px;">
 
   　　　　　　　 <h2>Safety</h2>
 
   　　　　　　　 <h2>Safety</h2>
 +
</div>
 
   　　　　　　　  <a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Safety" target="_blank"></a>
 
   　　　　　　　  <a href="https://2015.igem.org/Team:Tokyo-NoKoGen/Safety" target="_blank"></a>
−
  　　</div>
+
  　　</div>
 
   　　
 
   　　
 
        
 
        
Line 467: Line 467:
  
  
−
/*footer*/
+
<!--footer-->
 
<style>
 
<style>
 
div#wrapper2 {
 
div#wrapper2 {
Line 479: Line 479:
 
   
 
   
  
−
div#footer {
+
div#footer {
−
    margin:0 auto;
+
−
    position:absolute;
+
 
     width:900px;
 
     width:900px;
−
 
+
    position:relative;
−
　　left : -200px ;
+
    left:-12%;
−
     bottom:-1300px;
+
     bottom:-1200px;
 +
    background-color:#ffffff;
 +
    border-width: thick;
 +
border-color: #ADFF2F;
 +
border-style:inset;
 
         }
 
         }
−
 
+
div#footer img {
−
 
+
     height:50px;
−
div#footer img {
+
−
     width:200px;
+
 
         }
 
         }
 
p {
 
p {
Line 497: Line 497:
 
     line-height:2;  
 
     line-height:2;  
 
   }
 
   }
−
h2 {
+
 
−
    font-size:20px;
+
 
−
    font-family:fantasy;
+
</style>
−
    text-decoration:none;
+
−
  }
+
−
</style>
+
  
  
 
<body>
 
<body>
  
−
<div id="wrapper">
+
<div id="wrapper2">
 
<div id="footer">
 
<div id="footer">
 +
        <a href="http://www.asante.co.jp"><img src="https://static.igem.org/mediawiki/2015/a/a8/NK-image25.jpeg"></a>
 
<a href="http://www.idtdna.com/site"><img src="https://static.igem.org/mediawiki/2014/5/5e/Noko14_IDT.png"></a>
 
<a href="http://www.idtdna.com/site"><img src="https://static.igem.org/mediawiki/2014/5/5e/Noko14_IDT.png"></a>
−
<a href="http://www.ultizyme.jp/"><img src="https://static.igem.org/mediawiki/2014/c/ce/Noko14_Image91.jpg"></a>
+
<a href="http://www.ultizyme.jp/"><img src="https://static.igem.org/mediawiki/2014/c/ce/Noko14_Image91.jpg"></a><br>
 
<a href="http://www.cosmobio.co.jp/index_e.asp"><img src="https://static.igem.org/mediawiki/2014/e/e5/Noko14_Logo_e.jpg"></a>
 
<a href="http://www.cosmobio.co.jp/index_e.asp"><img src="https://static.igem.org/mediawiki/2014/e/e5/Noko14_Logo_e.jpg"></a>
 
<a href="http://www.mbl.co.jp/e/index.html"><img src="https://static.igem.org/mediawiki/2014/4/4f/Noko14_MBL.gif"></a>
 
<a href="http://www.mbl.co.jp/e/index.html"><img src="https://static.igem.org/mediawiki/2014/4/4f/Noko14_MBL.gif"></a>
Line 521: Line 519:
 
</body>
 
</body>
  
−
/*footer*/
+
<!--footer-->
 +
 
 +
 
 
</html>
 
</html>

Latest revision as of 18:32, 23 November 2015

　　
　 　　
　　　　　　　

Introduction

What kind image do you have when you hear the word “termite”??

Actually, this tiny insect has unimaginable voracity.
They cause great damage for human life, but there is no way to exterminate terites completely and safely.

To solve this problem,
Tokyo-NoKoGen “ExTermite Coli”, a new pesticides for Termites !!

　　　　　　　 　　　　　　　
　　　　
　　　　 　　　　 　　

Team

　 　　
　　

Protocol


　 　　
　 　　 　　 　　 　　 　
　　 　

project

The exterminateon of termites by ExTermite Coli involves three steps.
We use “pseudo egg” to distribute the engineered E.coli. Using this unique tool, the engineered E.coli carried into the termite’s body.
In the termite’s body, it produces toxic material to inhibit the metabolic pathway of the termites and exterminate termites. After extermination of the termites, ExTermite Coli loses its function by light-induced lysis.
On the project page, we explain about the details of our project, ExTermite Coli.

　　　　　　 　　　
　　　 　　　
　　 　　
　　　　　　　
　　　　　　　

Achievement

　　　　　　　 　　　　　　　 　　
　　　　　　　
　　 　　 　　
　　　　　　　
　　　　　　　

Human practice

　　　　　　　 　
　　　　　　　 　　
　　 　　
　　　　　　　
　　　　　　　

Parts

　　　　　　　 　　
　　 　　 　　
　　　　　　　
　　　　　　　

Safety