Difference between revisions of "Team:Tokyo-NoKoGen/Safety"

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<h2>Safety in iGEM</h2>
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h4>Safe Project Design</h4>
 
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
  
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h4>Safe Lab Work</h4>
 
  
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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          <m1>Safety</m1><br>
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    　<m2>------<b><u>What is the risk of our project?</b></u>-------<br></m2>
  
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<h4>Safe Shipment</h4>
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<m3>　　In our project, we expect the termites to eat the trehalase inhibitor, 3,3’-dkT, synthesized by our engineered <i>E. coli</i> on a solid medium. However, one of our possible problem is that <i>E. coli</i> on the surface of the termite’s body or legs may be carried outside of the trap and diffused. And termites have a habit to groom each other, so engineered <i>E. coli</i> can <u>diffuse from a termite to termites.</u><br>
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At the end, the <i>E. coli</i> may potentially spread in the environment and affect the ecosystem by acting on other insects!<br>
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　　To help prevent the unwanted spread of our engineered <i>E. coli</i>, we expect the future addition of a light-inducible lysis device. This lysis system would allow the <i>E. coli</i> to grow well and produce the insecticide only under the condition at colony in the dark. However, <i>E. coli</i> that exits at the termite’s surface would die once it’s exposed to light. A possible arrangement may involve placing <b>colicin-E1 under the control of the light inducible CcaS/CcaR two-component regulation system.</b><br></m3>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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<m2><u><b>Our training</b></u><br></m2>
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<m3>We have already had safety training.<br>
  
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　All members are given a detailed training about <u>basic lab safety</u>, for example, how to handle reagents and equipment (autoclave, burners, etc.) safely. Every week, we have a <u>short meeting to discuss any safety issues</u> that come up. We also learn how to handle genetically modified organisms during experiments and how to treat before disposing them.<br>
  
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><a href="http://www.tuat.ac.jp/~kankyou/index.shtml">Laboratory safety training requirements of our institution (Japanese only)</a><br>
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<br>
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<m2>Our local rules and regulations<br></m2>
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<m3>　Dr. Akinori Koukitu is responsible for the biological safety at our institution.<br>
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We submitted the details of our projects to him. We follow his advice.<br>
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><a href="http://www.tuat.ac.jp/~idenshi/Japanese%20Files/Kitei_Folder/kitei.html">Biosafety guidelines of our institution(Japanese only)</a><br>
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><a href="http://www.lifescience.mext.go.jp/bioethics/anzen.html#kumikae">The regulations that govern biosafety in research laboratories. (Japanese only)</a><br></m3>
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<br>
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<m2><u><b>Risks of our project</b></u><br></m2>
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<m3>　The safety and health of team members or other people working in the lab will <b>not be affected by our project.</b><br>
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　The only species we use is <i>Escherichia coli</i>, all of which are classified as risk group1 and they do not harm human health. <br>
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But <u>termites give the damage to buildings</u> <b>if they escaped from out cages.</b> We used termites carefully in the experiments so as not to escape to the outside. And we took care of the condition of living termite, <u><b>especially temperature.</b></u> Our using termites have a potential to get wings. So we had to keep them under 25 ℃ at all times.<br><br>
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　(1)To minimize risks to team members, we have a strict policy that anyone who enters the lab must wear goggles and lab coat, and anyone doing experiments also must wear gloves. Eating and drinking is strictly forbidden in our lab.<br>
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　(2)To minimize risks to public health and environment, we ensure that all biological materials are promptly autoclaved after using and disposed of appropriately. Furthermore, we follow our University and country regulations and guidelines for collecting and disposing of any toxic waste generated; our facility have already been equipped to handle such wastes.<br>
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　Biological materials and reagents used in our experiments are never brought outside the laboratory.<br>
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　(3)To minimize the risks of malicious misuse, the lab facilities are only unlocked when experiments are carried out, so our Biobrick parts and reagents cannot be stolen.<br></m3>
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Latest revision as of 16:11, 23 November 2015

　

Safety
　------What is the risk of our project?-------
 　　In our project, we expect the termites to eat the trehalase inhibitor, 3,3’-dkT, synthesized by our engineered E. coli on a solid medium. However, one of our possible problem is that E. coli on the surface of the termite’s body or legs may be carried outside of the trap and diffused. And termites have a habit to groom each other, so engineered E. coli can diffuse from a termite to termites.
At the end, the E. coli may potentially spread in the environment and affect the ecosystem by acting on other insects!
　　To help prevent the unwanted spread of our engineered E. coli, we expect the future addition of a light-inducible lysis device. This lysis system would allow the E. coli to grow well and produce the insecticide only under the condition at colony in the dark. However, E. coli that exits at the termite’s surface would die once it’s exposed to light. A possible arrangement may involve placing colicin-E1 under the control of the light inducible CcaS/CcaR two-component regulation system.
Our training
 We have already had safety training.
　All members are given a detailed training about basic lab safety, for example, how to handle reagents and equipment (autoclave, burners, etc.) safely. Every week, we have a short meeting to discuss any safety issues that come up. We also learn how to handle genetically modified organisms during experiments and how to treat before disposing them.
>Laboratory safety training requirements of our institution (Japanese only)

Our local rules and regulations
 　Dr. Akinori Koukitu is responsible for the biological safety at our institution.
We submitted the details of our projects to him. We follow his advice.
>Biosafety guidelines of our institution(Japanese only)
>The regulations that govern biosafety in research laboratories. (Japanese only)
Risks of our project
 　The safety and health of team members or other people working in the lab will not be affected by our project.
　The only species we use is Escherichia coli, all of which are classified as risk group1 and they do not harm human health.
But termites give the damage to buildings if they escaped from out cages. We used termites carefully in the experiments so as not to escape to the outside. And we took care of the condition of living termite, especially temperature. Our using termites have a potential to get wings. So we had to keep them under 25 ℃ at all times.

　(1)To minimize risks to team members, we have a strict policy that anyone who enters the lab must wear goggles and lab coat, and anyone doing experiments also must wear gloves. Eating and drinking is strictly forbidden in our lab.
　(2)To minimize risks to public health and environment, we ensure that all biological materials are promptly autoclaved after using and disposed of appropriately. Furthermore, we follow our University and country regulations and guidelines for collecting and disposing of any toxic waste generated; our facility have already been equipped to handle such wastes.
　Biological materials and reagents used in our experiments are never brought outside the laboratory.
　(3)To minimize the risks of malicious misuse, the lab facilities are only unlocked when experiments are carried out, so our Biobrick parts and reagents cannot be stolen.