Difference between revisions of "Team:Toronto/Composite Part"

 
(6 intermediate revisions by the same user not shown)
Line 13: Line 13:
 
<div id="navigation">
 
<div id="navigation">
 
<ul>
 
<ul>
−
<li><a href="https://2015.igem.org/Team:Toronto">home</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto"><span>home</span><i class="fa fa-home"></i></a></li>
−
<li><a href="https://2015.igem.org/Team:Toronto/Team">team</a></li>
+
</li>
−
<li><a href="#">project</a>
+
<li><a href="https://2015.igem.org/Team:Toronto/Team"><span>team</span><i class="fa fa-users"></i></a></li>
 +
</li>
 +
<li><a href="#"><span>project</span><i class="fa fa-info-circle"></i></a>
 
<ul>
 
<ul>
−
<li><a href="https://2015.igem.org/Team:Toronto/Description">description</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Description"><span>description</span></a></li>
−
<li><a href="https://2015.igem.org/Team:Toronto/Experiments">experiments</a></li>
+
</li>
−
<li><a href="https://2015.igem.org/Team:Toronto/Results">results</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Experiments"><span>experiments</span></a></li>
−
<li><a href="https://2015.igem.org/Team:Toronto/Design">design</a></li>
+
</li>
 +
<li><a href="https://2015.igem.org/Team:Toronto/Results"><span>results</span></a></li>
 +
</li>
 +
<li><a href="https://2015.igem.org/Team:Toronto/Design"><span>design</span></a></li>
 +
</li>
 
</ul>
 
</ul>
−
</li><li class="active"><a href="#">parts</a>
+
<li><a href="https://2015.igem.org/Team:Toronto/Parts"><span>parts</span><i class="fa fa-gear"></i></a></li>
 +
</li>
 +
<li><a href="https://2015.igem.org/Team:Toronto/Notebook"><span>notebook</span><i class="fa fa-book"></i></a></li>
 +
</li>
 +
<li><a href="https://2015.igem.org/Team:Toronto/Attributions"><span>attributions</span><i class="fa fa-life-saver"></i></a></li>
 +
</li>
 +
<li><a href="https://2015.igem.org/Team:Toronto/Collaborations"><span>collaborations</span><i class="fa fa-users"></i></a></li>
 +
</li>
 +
<li><a href="#"><span>practices</span><i class="fa fa-tree"></i></a>
 
<ul>
 
<ul>
−
<li><a href="https://2015.igem.org/Team:Toronto/Parts">parts</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Practices"><span>human practices</span></a></li>
−
<li><a href="https://2015.igem.org/Team:Toronto/Basic_Part">basic parts</a></li>
+
</li>
−
<li class="active"><a href="https://2015.igem.org/Team:Toronto/Composite_Part">composite parts</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Alberta-Oil-Stories"><span>alberta oil stories</span></a></li>
−
<li><a href="https://2015.igem.org/Team:Toronto/Part_Collection">part collection</a></li>
+
</li>
 +
<li><a href="https://2015.igem.org/Team:Toronto/Community-Outreach"><span>community outreach</span></a></li>
 +
</li>
 +
<li><a href="https://2015.igem.org/Team:Toronto/Technology-Applications"><span>technology applications</span></a></li>
 +
</li>
 +
<li><a href="https://2015.igem.org/Team:Toronto/TechnoMoral-Scenarios"><span>technoMoral scenarios</span></a></li>
 +
</li>
 
</ul>
 
</ul>
−
</li><li><a href="https://2015.igem.org/Team:Toronto/Notebook">notebook</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Safety"><span>safety</span><i class="fa fa-fire"></i></a></li>
−
<li><a href="https://2015.igem.org/Team:Toronto/Attributions">attributions</a></li>
+
</li>
−
<li><a href="https://2015.igem.org/Team:Toronto/Collaborations">collaborations</a></li>
+
<li><a href="#"><span>software</span><i class="fa fa-code"></i></a>
−
<li><a href="https://2015.igem.org/Team:Toronto/Practices">human practices</a></li>
+
<ul>
−
<li><a href="https://2015.igem.org/Team:Toronto/Safety">safety</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Software"><span>software</span></a></li>
−
<li><a href="https://2015.igem.org/Team:Toronto/Modeling">modeling</a></li>
+
</li>
−
<li><a href="https://2015.igem.org/Team:Toronto/Measurement">measurement</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Demo"><span>demo</span></a></li>
−
<li><a href="https://2015.igem.org/Team:Toronto/Software">software</a></li>
+
</li>
−
<li><a href="https://2015.igem.org/Team:Toronto/Entrepreneurship">entrepreneurship</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Generator"><span>wiki generator</span></a></li>
 +
</li>
 +
</ul>
 +
<li><a href="https://2015.igem.org/Team:Toronto/Entrepreneurship"><span>entrepreneurship</span><i class="fa fa-institution"></i></a></li>
 +
</li>
 
</ul>
 
</ul>
 
</div>
 
</div>
−
<div class="content">
+
<div class="content" id="content-main"><div class="row"><div class="col col-lg-8 col-md-12"><div class="content-main"></div></div><div id="tableofcontents" class="tableofcontents affix sidebar col-lg-4 hidden-xs hidden-sm hidden-md visible-lg-3"><ul class="nav"></div></div></div>
−
<h2 id="tode-and-todf-plasmid-design">TodE and TodF Plasmid Design</h2>
+
−
<p>Pseudomonas Putida F1 species degrade toluene with the help of tod operon. Toluene dioxygenase and toluene cis-dihydrodiol dehydroxygenase transcribed from transcribed from todC1C2BA and todD genes respectively facilitate break down of toluene to 3-methylcatechol. Gerben J. Zylstra et al successfully constructed the plasmid pTDG602 containing todC1C2BAD genes. To achieve complete degradation of toluene down to carbon dioxide and water, we focused on designing a plasmid containing todE and todF genes. After cotransformation of DH10B cells with pTDG602 and our plasmid, E.Coli theoretically should contain all the genes required to achieve complete degradation.</p>
+
−
<p><img src="https://github.com/igemuoftATG/wiki2015/blob/master/images/Pathway.png" alt="alt text"></p>
+
−
<p>In order to achieve the best protein production efficiency, we codon optimized our todE and todF sequences. We used Salis RBS calculator to optimize translation efficiency. We used a tunable promoter to determine optimum transcription for DH10B cells.</p>
+
−
<p><img src="https://github.com/igemuoftATG/wiki2015/blob/master/images/biobrick%20plasmid.png" alt="alt text"></p>
+
−
<p>We also used BioBricks in the registry to design plasmids with different efficiency by using RDP kit as shown below. In this process, we used promoter BBa_J23102 and RBS BBa_B0030.</p>
+
−
<p><img src="file://localhost/Users/joannadowdell/Downloads/TodEplasmid.webp" alt="alt text"></p>
+
−
<p><img src="https://github.com/igemuoftATG/wiki2015/blob/master/images/TodFplasmid.png" alt="alt text"></p>
+
−
<p>Note: Links to iGem registry
+
−
BBa_J23102: <a href="http://parts.igem.org/Part:BBa_J23102">http://parts.igem.org/Part:BBa_J23102</a>
+
−
BBa_0030: <a href="http://parts.igem.org/Part:BBa_B0030">http://parts.igem.org/Part:BBa_B0030</a></p>
+
−
 
+
−
</div>
+
 
</html>
 
</html>
 
{{Toronto/footer}}
 
{{Toronto/footer}}

Latest revision as of 04:47, 20 November 2015