Difference between revisions of "Team:Toulouse/Safety"

Line 13: Line 13:
  
 
<script>
 
<script>
−
var myurl = "https://2015.igem.org/Team:Toulouse/project/strat";
+
var myurl = "https://2015.igem.org/Team:Toulouse/Descrition";
 
var elementId = "project";
 
var elementId = "project";
 
</script>
 
</script>
Line 23: Line 23:
 
   
 
   
 
     <!-- ################################################################################################  
 
     <!-- ################################################################################################  
−
<div class="wrapper row2 bgded" style="background-image:url('https://static.igem.org/mediawiki/2015/0/03/TLSE_bakcground.png');">
+
 
−
    <div id="pageintro">
+
−
<div class="title">
+
−
      <h2>Context</h2>
+
−
 
+
−
    </div>
+
−
+
−
    <div id="breadcrumb" class="clear">
+
−
      <ul>
+
−
        <li><a href="#domBee">1- Domestic bees</a></li>
+
−
        <li><a href="#CCD">2- Collapse of Bees</a></li>
+
−
        <li><a href="#varroa"><i>3- Varroa destructor</i></a></li>
+
−
        <li><a href="#outB">4- Outbreak of Varroa</a></li>
+
−
<li><a href="#fight">5- Means to fight Varroa</a></li>
+
−
      </ul>
+
−
    </div>
+
−
</div>
+
−
</div>
+
 
     <!-- ################################################################################################ -->
 
     <!-- ################################################################################################ -->
 
    
 
    
Line 47: Line 30:
 
<!-- ################################################################################################ -->
 
<!-- ################################################################################################ -->
 
<!-- ################################################################################################ -->
 
<!-- ################################################################################################ -->
−
<div class="wrapper row0 bgded" style="background-image:url('https://static.igem.org/mediawiki/2015/0/03/TLSE_bakcground.png')">
+
<div class="wrapper row0 bgded" style="background-image:url('https://static.igem.org/mediawiki/2015/3/33/TLSE_bg_7.png')">
 
    
 
    
  
Line 56: Line 39:
 
     <div class="shout-content clear">
 
     <div class="shout-content clear">
 
       <div class="title">
 
       <div class="title">
−
     <center> <h3>Strategy</h3> </center>
+
     <center> <h3>Safety</h3> </center>
 
     </div>
 
     </div>
−
  <center><img src="https://static.igem.org/mediawiki/2015/b/b9/TLSE_strategy_BG.png"></center>
+
  <center><img src="https://static.igem.org/mediawiki/2015/6/67/TLSE_BG.png"></center>
 
    
 
    
 
</div>
 
</div>
Line 75: Line 58:
 
<br>
 
<br>
 
   <main class="container clear">  
 
   <main class="container clear">  
−
   
+
 
 +
 
 +
  <div class="title">
 +
      <a href="#main1"><h3>Content</h3></a>
 +
    </div>
 
 
−
   
+
<center>
−
    <!-- container body -->
+
−
    <!-- ################################################################################################ -->
+
−
 
+
−
 
+
−
    <!-- ################################################################################################ -->
+
−
    <div class="title">
+
−
      <h3>Content</h3>
+
−
    </div>
+
−
<center>
+
 
     <div id="breadcrumb" class="clear" style="float: center;" >
 
     <div id="breadcrumb" class="clear" style="float: center;" >
 
  <ul>
 
  <ul>
−
         <li><a href="#part1">- Introducing <i>ApiColi</i></a></li>
+
         <li><a href="#part1">- INSA safety training</a></li>
−
         <li><a href="#part2">- It’s a TrapiColi!</a></li>
+
         <li><a href="#part2">- Safety in the lab</a></li>
−
         <li><a href="#part3">- <i>Apicoli</i> circadian rythm</a></li>
+
         <li><a href="#part3">- Attraction test on varroas</a></li>
 +
        <li><a href="#part4">- Mortality test on varroas</a></li>
 
       </ul>
 
       </ul>
 
     </div>
 
     </div>
−
 
−
<hr style="width:66%;height:1px;border:none;color:rgba(29, 5, 79, 1);background-color:rgba(29, 5, 79, 1);">
 
 
</center>
 
</center>
−
  <!--  ################################################################################################ -->
 
 
    
 
    
−
 
+
  <center><div class="title" >    
−
    <!-- FIRST PARAGRAPH -->
+
<h3>Team safety and training</h3>
−
<center><div class="subtitle" >    
+
−
<h3>An ounce of prevention is worth a pound of cure
+
−
</h3>
+
 
</div></center>
 
</div></center>
 +
 +
<div class="subtitle" >  
 +
<h3>INSA safety training</h3>
 +
</div>
  
 
<div class="group center">
 
<div class="group center">
−
<div class="tow_third first">
+
<p align="justify" style="font-size:15px;">
−
        <p align="justify" style="font-size:15px;">
+
During the summer, our lab was in the engineering school INSA wich posses a
 +
safety department with one prevention advisor and several prevention assistants assisting the laboratories.
 +
Their goal is to ensure the well being of the employees regarding safety rules and risk prevention.
 +
Our laboratory, the LISBP is assisted by Nathalie Doubrovine who assisted us regarding safety procedures.
  
−
+
As new interns we had to follow several trainings and tests to learn how to identify the risks and prevents
−
Given the
+
ourselves and our colleagues from harm.<br>
−
influence of the varroa mite in bee population decline,
+
We also followed several other training that allowed us to use technical apparatus such as autoclave or MNR.
−
we aimed our project to fit into the fight against varroa.
+
<i>The laboratory safety training requirements of the LISBP are detailed into the Rules of Procedures of the LISBP</i>
−
<b>Current chemical treatments</b> used to fight varroa are not
+
</p>
−
satisfying since they are <b>harmful for bees</b> and human health,
+
</div>
−
and beekeepers relate a <b>lack of effectiveness</b>.
+
 
−
</p><div id="part1"><!-- ANCHOR 1--></div>  
+
<div class="subsubtitle" >  
 +
<h3>New employees safety training</h3>
 +
</div>
 +
<div class="group center">
 
<p align="justify" style="font-size:15px;">
 
<p align="justify" style="font-size:15px;">
−
Our project, <i>ApiColi</i>, is an <b>alternative solution</b> in the  
+
Every new LISBP employee has to make this training regardless of its status (researcher, PhD student, intern, etc...).
−
fight against varroa in order to <b>establish balance</b>  
+
The training is divided into two parts, the first one is a training concerning general risk prevention in research laboratories.
−
between <i>Apis mellifera</i> and <i>Varroa destructor</i>
+
This one is made individually with the NEO software and with the explanations of the prevention assistant. It also informs about
−
and thus contribute to the preservation of ecosystems.
+
the emergency numbers to call.<br>
 +
The second one is a training about the techniques used during our stay concerning microbiological, chemical and incendiary risks.
 +
<br>
 +
We then answered a test to ensure we listened and understood the previous presentation.  
 
</p>
 
</p>
 
</div>
 
</div>
  
 +
<div class="subsubtitle" >  
 +
<h3>Autoclave</h3>
 +
</div>
 +
<div class="group center">
 +
<p align="justify" style="font-size:15px;">
 +
The whole team went through an autoclave training, detailing the explosive/implosive danger surrounding working with an under pressure apparel.<br>
 +
A lab coat, heat resistant gloves and glasses must be worn when manipulating the autoclave.
 +
</p>
 +
</div>
  
−
 
+
<div class="subsubtitle" >  
−
<div class="one_third">
+
<h3>Liquid nitrogen</h3>
−
<img src="https://static.igem.org/mediawiki/2015/1/1b/TLSE_WIP.png" style="width:70%;"/>
+
 
</div>
 
</div>
 +
<div class="group center">
 +
<div class="one_half first">
 +
<p align="justify" style="font-size:15px;">
 +
We also received a training explaining the risks of manipulating liquid nitrogen and how to manage incidents.
 +
Isothermic gloves, glasses and of course a lab coat must be worn when using it.
  
−
      </div>
+
</p>
−
  <div class="subtitle">  
+
</div>
−
<h3>Introducing <i>ApiColi</i>
+
−
</h3>
+
 
</div>
 
</div>
  
 +
<div class="subsubtitle" >  
 +
<h3>MNR</h3>
 +
</div>
 
<div class="group center">
 
<div class="group center">
−
        <p align="justify" style="font-size:15px;">
+
<div class="one_half first">
−
+
<p align="justify" style="font-size:15px;">
−
Our strategy is based on the <b>alternate production</b> of two molecules according to a <b>circadian cycle</b>, and thanks to a genetically modified <i>Escherichia coli</i> strain. <br>
+
  
−
<b>During the day</b>, while bees are entering or exiting the beehive, <b>butyrate</b> is biosynthesized by our strain, <i>ApiColi</i>, in order to <b>attract the varroa</b> which is fixed on bees. <br>
+
</p>
 +
</div>
 +
</div>
  
−
<b>By night</b>, <i>ApiColi</i> produces <b>formate</b>, a well-known molecule <b>lethal to the varroa</b> attracted during the day.<br>
+
<div class="subsubtitle" >  
 +
<h3>Legislation and French Labor Law</h3>
 +
</div>
 +
<div class="group center">
  
−
Formic acid is currently used to fight varroa but at <b>very high doses</b> that also have an <b>impact on bees</b>.
+
</div>
  
−
</p>
+
  <center><div class="title" >  
−
      </div>
+
<h3>Safety in the lab</h3>
−
+
</div></center>
−
 
+
−
 
+
−
  <div class="group center">
+
−
<br>
+
−
  <img src="https://static.igem.org/mediawiki/2015/3/38/TLSE_strategy_fig1.png" style="width:66%;height:580px;" />
+
−
</div>
+
−
<div id="part2"><!-- ANCHOR 2--></div>
+
−
<div class="group center">
+
−
<br>
+
−
<p> Figure 1: Circadian rhythm switch strategy</p>
+
−
</div>
+
  
 +
  <div class="subtitle" >  
 +
<h3>Equipment</h3>
 +
</div>
  
 +
<div class="group center">
  
−
<div class="subtitle">  
+
</div>
−
<h3>It’s a TrapiColi!
+
  
−
</h3>
+
<div class="subtitle" >  
 +
<h3>Waste</h3>
 
</div>
 
</div>
  
 
<div class="group center">
 
<div class="group center">
−
        <p align="justify" style="font-size:15px;">
+
 
−
In our project, the engineered bacteria <i>ApiColi</i> will be placed at the bottom of a trap, called <b>TrApiColi</b>,
+
−
positioned at the entrance of the hive. Varroas will be <b>attracted</b> and killed there, leaving the <b>bee colony
+
−
less exposed </b>to both chemicals, and particularly formic acid.
+
−
</p>
+
 
</div>
 
</div>
−
<div id="part3"><!-- ANCHOR 3--></div>
+
 
 +
<div class="subtitle" >  
 +
<h3>Storage</h3>
 +
</div>
 +
 
 
<div class="group center">
 
<div class="group center">
−
  <p align="justify" style="font-size:15px;">
 
−
Furthermore, at the moment, beehives are only treated with formic acid during <b>spring</b> and <b>fall</b>, when no
 
−
honey is being made. This is due to the fact that formic acid weakens bees but also that when varroa have entered the
 
−
brood, they are <b>not affected by it</b>. <br>
 
−
The attraction power of <i>ApiColi</i> will enable us to prevent most varroas from entering the beehive and reaching the brood.
 
−
This way, our treatment would be <b>useable</b> even <b>during the summer</b>.
 
−
</p>
 
−
      </div>
 
  
−
<div class="subtitle">  
+
</div>
−
<h3><i>Apicoli</i> circadian rythm
+
  
−
</h3>
+
<div class="subtitle" >  
 +
<h3>Rules</h3>
 
</div>
 
</div>
  
 
<div class="group center">
 
<div class="group center">
−
        <p align="center" style="font-size:15px;">
+
 
−
The synthetic regulation system used in <i>ApiColi</i> is based on a <b>chimeric red light receptor</b> that makes our bacterium <b>sensible to daylight</b>. <br>
+
−
</p>
+
 
</div>
 
</div>
  
−
<div class="group center">
+
<div class="subtitle" >  
−
<div class="one_half first">
+
<h3>Ethidium Bromide</h3>
−
<img src="https://static.igem.org/mediawiki/2015/5/57/TLSE_Attract_BG.png" style="width:40%;" />
+
 
</div>
 
</div>
−
<div class="one_half">
+
 
−
<br>
+
<div class="group">
−
<br>
+
 
−
<br>
+
−
<br>
+
−
<br>
+
−
<h3 class="nospace"><a target="_blank" href="https://2015.igem.org/Team:Toulouse/project/attract">During the day</a></h3>
+
−
            <p align="center" style="font-size:15px;">Formate expression is repressed and <b>butyrate synthesis</b> inhibition is stopped.
+
−
<br>Thus <i>Apicoli</i> produces only <b>butyrate</b>.
+
−
</p>
+
−
</div>
+
−
</div>
+
−
<div class="group center">
+
 
<div class="one_half first">
 
<div class="one_half first">
−
<img src="https://static.igem.org/mediawiki/2015/0/0b/TLSE_Eradicate_BG.png" style="width:60%;" />
+
<center>
 +
<div class="subsubtitle" >  
 +
<h3>Dark room</h3>
 +
</div>
 +
</center>
 
</div>
 
</div>
 +
 
<div class="one_half">
 
<div class="one_half">
−
<br>
+
<center>
−
<br>
+
<div class="subsubtitle" >  
−
<br>
+
<h3>Waste</h3>
−
<br>
+
</div>  
−
<br>
+
</center>
−
<h3 class="nospace"><a target="_blank" href="https://2015.igem.org/Team:Toulouse/project/eradicate">During the night</a></h3>
+
−
            <p align="center" style="font-size:15px;">The system reverses, and <b>formate</b> is synthesized while butyrate synthesis is inhibited.
+
−
 
+
−
</p>
+
−
</div>
+
 
</div>
 
</div>
 +
  
  
 
</div>
 
</div>
 +
 
   </main>
 
   </main>
 
</div>
 
</div>
Line 254: Line 232:
 
<br>
 
<br>
 
<div class="clear">
 
<div class="clear">
−
<ul>
 
−
<li>
 
−
[1] Yves Le Conte, Marion Ellis, Wolfgang RITTER. Varroa mites and honey bee health: can Varroa explain part of the colony losses? Apidologie, Springer Verlag (Germany), 2010, 41 (3), <10.1051/apido/2010017>.</li>
 
−
 
−
<li>
 
−
[2] Sammataro, D., Gerson, U., Needham, G., 2000. Parasitic mites of honey bees life history implications and impact. Annual Review of Entomology 45, 519-548
 
−
</li>
 
−
 
−
<li>
 
−
[3] Peng, Y-S., Fang, Y., Xu, S., Ge, L., 1987. The resistance mechanism of the Asian honey bee, Apis cerana Fabr., to an ectoparasitic mite, Varroa jacobsoni Oudemans. Journal of invertebrate pathology 49, 54-60.
 
−
</li>
 
−
 
−
<li>
 
−
[4] S, L, P Wendling. 2012. Varroa destructor (ANDERSON ET TRUEMAN, 2000), UN ACARIEN ECTOPARASITE DE L’ABEILLE DOMESTIQUE Apis mellifera LINNAEUS, 1758. REVUE BIBLIOGRAPHIQUE ET CONTRIBUTION À L’ÉTUDE DE SA REPRODUCTION.</li>
 
  
−
</ul>
 
 
</div>
 
</div>
 
<br>
 
<br>

Revision as of 12:19, 3 September 2015

iGEM Toulouse 2015

Safety


Content

Team safety and training

INSA safety training

During the summer, our lab was in the engineering school INSA wich posses a safety department with one prevention advisor and several prevention assistants assisting the laboratories. Their goal is to ensure the well being of the employees regarding safety rules and risk prevention. Our laboratory, the LISBP is assisted by Nathalie Doubrovine who assisted us regarding safety procedures. As new interns we had to follow several trainings and tests to learn how to identify the risks and prevents ourselves and our colleagues from harm.
We also followed several other training that allowed us to use technical apparatus such as autoclave or MNR. The laboratory safety training requirements of the LISBP are detailed into the Rules of Procedures of the LISBP

New employees safety training

Every new LISBP employee has to make this training regardless of its status (researcher, PhD student, intern, etc...). The training is divided into two parts, the first one is a training concerning general risk prevention in research laboratories. This one is made individually with the NEO software and with the explanations of the prevention assistant. It also informs about the emergency numbers to call.
The second one is a training about the techniques used during our stay concerning microbiological, chemical and incendiary risks.
We then answered a test to ensure we listened and understood the previous presentation.

Autoclave

The whole team went through an autoclave training, detailing the explosive/implosive danger surrounding working with an under pressure apparel.
A lab coat, heat resistant gloves and glasses must be worn when manipulating the autoclave.

Liquid nitrogen

We also received a training explaining the risks of manipulating liquid nitrogen and how to manage incidents. Isothermic gloves, glasses and of course a lab coat must be worn when using it.

MNR

Legislation and French Labor Law

Safety in the lab

Equipment

Waste

Storage

Rules

Ethidium Bromide

Dark room

Waste

References