Difference between revisions of "Team:Toulouse/Safety"

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    <center> <h3>Safety</h3> </center>
 
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      <a href="#main1"><h3>Content</h3></a>
 
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  <ul>
 
        <li><a href="#part1">- INSA safety training</a></li>
 
        <li><a href="#part2">- Safety in the lab</a></li>
 
        <li><a href="#part3">- Attraction test on varroas</a></li>
 
        <li><a href="#part4">- Mortality test on varroas</a></li>
 
      </ul>
 
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  <center><div class="title" >  
 
<h3>Team safety and training</h3>
 
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<div class="subtitle" >  
 
<h3>INSA safety training</h3>
 
</div>
 
 
<div class="group center">
 
<p align="justify" style="font-size:15px;">
 
During the summer, our lab was in the engineering school INSA wich posseses a
 
safety department with one prevention advisor and several prevention assistants assisting the laboratories.
 
Their goal is to ensure the well being of the employees regarding safety rules and risk prevention.
 
Our laboratory, the LISBP is assisted by Nathalie Doubrovine who assisted us regarding safety procedures.
 
 
As new interns we had to follow several trainings and tests to learn how to identify the risks and prevents
 
ourselves and our colleagues from harm.<br>
 
We also followed several other training that allowed us to use technical apparatus such as autoclave or MNR.<br>
 
<i>The laboratory safety training requirements of the LISBP are detailed into the Rules of Procedures of the LISBP</i>
 
</p>
 
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<div class="subsubtitle" >  
 
<h3>New employees safety training</h3>
 
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<p align="justify" style="font-size:15px;">
 
Every new LISBP employee has to make this training regardless of its status (researcher, PhD student, intern, etc...).
 
The training is divided into two parts, the first one is a training concerning general risk prevention in research laboratories.
 
This one is made individually with the NEO software and with the explanations of the prevention assistant. It also informs about
 
the emergency numbers to call.<br>
 
The second one is a training about the techniques used during our stay concerning microbiological, chemical and incendiary risks.
 
<br>
 
We then answered a test to ensure we listened and understood the previous presentation.
 
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<h3>Autoclave</h3>
 
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<p align="justify" style="font-size:15px;">
 
The whole team went through an autoclave training, detailing the explosive/implosive danger surrounding work with an under pressure apparel.<br>
 
A lab coat, heat resistant gloves and glasses must be worn when manipulating the autoclave.
 
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<h3>Liquid nitrogen</h3>
 
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<p align="justify" style="font-size:15px;">
 
We also received a training explaining the risks of manipulating liquid nitrogen and how to manage incidents.
 
Isothermic gloves, glasses and of course a lab coat must be worn when using it.
 
 
</p>
 
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<div class="subsubtitle" >  
 
<h3>MNR</h3>
 
</div>
 
<div class="group center">
 
<div class="one_half first">
 
<p align="justify" style="font-size:15px;">
 
 
</p>
 
</div>
 
</div>
 
 
<div class="subsubtitle" >  
 
<h3>Legislation and French Labor Law</h3>
 
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<div class="group center">
 
<p align="justify" style="font-size:15px;">
 
INSA Toulouse is a public school for engineers. The biosafety guidelines are not specific to our institution; the French National regulations for working conditions and for manipulating genetically modified organisms are applied.<br>
 
The regulation about the workers' prevention against risks resulting from their exposure to pathogenic biological agents (Decree No. 94-352 of 4 May 1994) includes microorganisms, cell cultures and human endoparasites which may cause infections, allergies or toxicity. <br>
 
This Decree is the French transposition of the Directive 90/679 / EEC and is also transcribed in the Labour Code (Articles L4421-1 R4421-1 to R4427-5.)<br>
 
The <a href="http://www.legifrance.gouv.fr/affichTexte.do?cidTexte=JORFTEXT000000465273&dateTexte=&categorieLien=id">Decree of the 16th July 2007</a> describes the technical preventive measuresto set up in research laboratories (including containment), education, analysis, anatomy and surgical pathology, autopsy rooms, and industrial and agricultural facilities where workers are likely to be exposed to biological pathogens.<br>
 
The rules of health, safety, and preventive medicine applied in public services in France (and thus in all public facilities working in scientific and technological domains) are set out in the <a href="http://www.legifrance.gouv.fr/affichTexte.do?cidTexte=LEGITEXT000006063791">Decree No. 82-453</a>. This decree refers to the Labour Code, Public Health Code and Environmental Code.<br>
 
The <a href="http://legifrance.gouv.fr/affichTexte.do?cidTexte=JORFTEXT000024584737&categorieLien=id">Decree No. 2011-1177</a> is related to the use of genetically modified organisms.<br>
 
<br>
 
</p>
 
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  <center><div class="title" >  
 
<h3>Safety in the lab</h3>
 
 
 
  <div class="subtitle" >  
 
<h3>Equipment</h3>
 
</div>
 
 
<div class="group center">
 
<ul align="left" style="font-size:15px;">
 
<li>A conventional lab coat, closed with long sleeves</li>
 
<li>Closed shoes</li>
 
<li>Gloves</li>
 
<li>Glasses if needed (UV exposure, hot water or chemical handling)</li>
 
</ul>
 
</div>
 
 
<div class="subtitle" >  
 
<h3>Waste</h3>
 
</div>
 
<div class="group center">
 
<table style="font-size:15px;" style="td align='left'">
 
 
          <tr>
 
            <th colspan=2><p>Different trash containers are available in the lab</p></th>
 
          </tr>
 
     
 
          <tr>
 
            <td><p>One for biological waste (yellow). This waste will be autoclaved before being thrown out</p></td>
 
<td><p><img src="https://static.igem.org/mediawiki/2015/6/6e/TLSE_safety_fig1.png" /></p></td>
 
          </tr>
 
          <tr>
 
            <td><p>One for common waste (green or orange)</p></td>
 
<td><p>PLACE PICTURE HERE</p></td>
 
          </tr>
 
     
 
      </table>
 
  </div>
 
</table>
 
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<div class="subtitle" >  
 
<h3>Storage</h3>
 
</div>
 
<div class="group center">
 
<div class="group center">
 
<table class="df" style="font-size:15px;" align="left">
 
 
          <tr>
 
            <th colspan=2><p>Different trash containers are available in the lab</p></th>
 
          </tr>
 
     
 
          <tr>
 
            <td><p>One for biological waste (yellow). This waste will be autoclaved before being thrown out</p></td>
 
<td><p><img src="https://static.igem.org/mediawiki/2015/6/6e/TLSE_safety_fig1.png" /></p></td>
 
          </tr>
 
          <tr>
 
            <td><p>One for common waste (green or orange)</p></td>
 
<td><p>PLACE PICTURE HERE</p></td>
 
          </tr>
 
     
 
      </table>
 
  </div>
 
</table>
 
</div>
 
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<div class="subtitle" >  
 
<h3>Rules</h3>
 
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<div class="group center">
 
 
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<h3>Ethidium Bromide</h3>
 
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<h3>Dark room</h3>
 
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<div class="subsubtitle" >  
 
<h3>Waste</h3>
 
</div>
 
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Latest revision as of 20:30, 17 September 2015