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| <h1 align="center">Main Goal</h1> | | <h1 align="center">Main Goal</h1> |
| <p aling="center">For the iGEM competition, the team aims to engineer a biological system, enabling it to degrade glucose in order to produce and export into the medium a biodegradable plastic called PLA.</p> | | <p aling="center">For the iGEM competition, the team aims to engineer a biological system, enabling it to degrade glucose in order to produce and export into the medium a biodegradable plastic called PLA.</p> |
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| <table> | | <table> |
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| <tr> | | <tr> |
| <td>[1]</td> | | <td>[1]</td> |
− | <td><p>El Banco Mundial, 2014. Una bolsa de plástico para asfixiar el mar. [online] <http://www.bancomundial.org/es/news/feature/2014/12/08/bolsa-de-plastico-asfixiar-planeta> [consulted: 14-07-2015]<p/></td> | + | <td>El Banco Mundial, 2014. Una bolsa de plástico para asfixiar el mar. [online] <http://www.bancomundial.org/es/news/feature/2014/12/08/bolsa-de-plastico-asfixiar-planeta> [consulted: 14-07-2015]</td> |
| </tr> | | </tr> |
| | | |
| <tr> | | <tr> |
| <td>[2]</td> | | <td>[2]</td> |
− | <td><p>Garlotta, 2002. A Literature Review of PolyLactic Acid. Journal of Polymers and the Environment, Vol. 9, No. 2.</p></td> | + | <td>Garlotta, 2002. A Literature Review of PolyLactic Acid. Journal of Polymers and the Environment, Vol. 9, No. 2.</td> |
| </tr> | | </tr> |
| | | |
| <tr> | | <tr> |
− | <td>HTML</td> | + | <td>[3] </td> |
− | <td>20</td> | + | <td> Alla Katsnelson. News Feature: Microplastics present pollution puzzle. Proceedings of the National Academy of Sciences (2015) vol. 112 no. 18, p5547-5549</td> |
− | <td>16:00 - 20:00</td>
| + | |
| </tr> | | </tr> |
| | | |
| <tr> | | <tr> |
− | <td>Dreamweaver</td> | + | <td>[4] </td> |
− | <td>60</td> | + | <td> El Tiempo, 2014. Plásticos matan al año 1,5 millones de animales marinos. [online] <http://www.eltiempo.com/estilo-de-vida/ciencia/muerte-de-animales-por-plasticos-lanzados-al-mar/14710998> [consulted: 14-07-2015]</td> |
− | <td>16:00 - 20:00</td>
| + | |
| </tr> | | </tr> |
− | </table>
| |
| | | |
| + | <tr> |
| + | <td>[5] </td> |
| + | <td>La Tercera, 2015. Cristina Espinoza. Hasta 25 mil toneladas de plástico anuales se arrojan al mar desde Chile. [online] <http://www.latercera.com/noticia/tendencias/2015/05/659-627978-9-hasta-25-mil-toneladas-de-plasticos-anuales-se-arrojan-al-mar-desde-chile.shtml> [consulted: 14-07-2015] </td> |
| + | </tr> |
| | | |
| + | <tr> |
| + | <td>[6] </td> |
| + | <td> 5 Gyres. [online] <http://www.5gyres.org/the-plastic-problem> [consulted: 13-09-2015]</td> |
| + | </tr> |
| | | |
| + | <tr> |
| + | <td>[7] </td> |
| + | <td> Chilean Senate, 2014. [online] <http://www.senado.cl/prohibicion-de-bolsas-plasticas-en-la-patagonia-votaran-idea-de-legislar/prontus_senado/2014-10-23/122842.html> [consulted: 14-07-2015]</td> |
| + | </tr> |
| | | |
| + | <tr> |
| + | <td>[8] </td> |
| + | <td> PlasticsEurope. Plásticos - Situación en 2011. Análisis de la producción, la demanda y la recuperación de plásticos en Europa en 2010. [online] <http://www.plasticseurope.org/documents/document/20111107102611-pe_factsfigures_es_2011_lr_final041111.pdf> [consulted: 15-07-2015]</td> |
| + | </tr> |
| | | |
− | <br>[3] Alla Katsnelson. News Feature: Microplastics present pollution puzzle. Proceedings of the National Academy of Sciences (2015) vol. 112 no. 18, p5547-5549 | + | <tr> |
− | <br>[4] El Tiempo, 2014. Plásticos matan al año 1,5 millones de animales marinos. [online] <http://www.eltiempo.com/estilo-de-vida/ciencia/muerte-de-animales-por-plasticos-lanzados-al-mar/14710998> [consulted: 14-07-2015] | + | <td>[9] </td> |
| + | <td> Serna et al. Ácido Poliláctico (PLA): Propiedades y Aplicaciones. Ingeniería y Competitividad (2003), Vol.5, 16-26.</td> |
| + | </tr> |
| | | |
− | <br>[5] La Tercera, 2015. Cristina Espinoza. Hasta 25 mil toneladas de plástico anuales se arrojan al mar desde Chile. [online] <http://www.latercera.com/noticia/tendencias/2015/05/659-627978-9-hasta-25-mil-toneladas-de-plasticos-anuales-se-arrojan-al-mar-desde-chile.shtml> [consulted: 14-07-2015] | + | <tr> |
| + | <td>[10] </td> |
| + | <td>The Economist. The price of making a plastic bottle. 2014 <http://www.economist.com/news/economic-and-financial-indicators/21632569-price-making-plastic-bottle> [consulted: 13-09-2015] </td> |
| + | </tr> |
| | | |
− | <br>[6] 5 Gyres. [online] <http://www.5gyres.org/the-plastic-problem> [consulted: 13-09-2015] | + | <tr> |
| + | <td>[11] </td> |
| + | <td> Facts on Pet. Husky's guide to PET Bottles. http://www.factsonpet.com/Articles/Facts%20on%20PET%20Flyer_June18%20PRINT.pdf [consulted: 13-09-2015]</td> |
| + | </tr> |
| | | |
− | <br>[7] Chilean Senate, 2014. [online] <http://www.senado.cl/prohibicion-de-bolsas-plasticas-en-la-patagonia-votaran-idea-de-legislar/prontus_senado/2014-10-23/122842.html> [consulted: 14-07-2015] | + | <tr> |
| + | <td>[12] </td> |
| + | <td> The Field Position. The Importance of Corn.2012. < http://www.thefieldposition.com/2012/06/the-importance-of-corn/> [consulted: 13-09-2015]</td> |
| + | </tr> |
| | | |
− | <br>[8] PlasticsEurope. Plásticos - Situación en 2011. Análisis de la producción, la demanda y la recuperación de plásticos en Europa en 2010. [online] <http://www.plasticseurope.org/documents/document/20111107102611-pe_factsfigures_es_2011_lr_final041111.pdf> [consulted: 15-07-2015] | + | <tr> |
| + | <td>[13] </td> |
| + | <td> Yale iGEM Team 2013. <https://2013.igem.org/Team:Yale> [consulted: 13-09-2015]</td> |
| + | </tr> |
| | | |
− | <br>[9] Serna et al. Ácido Poliláctico (PLA): Propiedades y Aplicaciones. Ingeniería y Competitividad (2003), Vol.5, 16-26. | + | <tr> |
| + | <td>[14] </td> |
| + | <td> Jung et al. Metabolic Engineering of Escherichia coli for the Production of Polylactic Acid and Its Copolymers. (2010) Biotechnology and Bioengineering, Vol. 105, No. 1</td> |
| + | </tr> |
| | | |
− | <br>[10] The Economist. The price of making a plastic bottle. 2014 <http://www.economist.com/news/economic-and-financial-indicators/21632569-price-making-plastic-bottle> [consulted: 13-09-2015] | + | <tr> |
− | <br>[11] Facts on Pet. Husky's guide to PET Bottles. http://www.factsonpet.com/Articles/Facts%20on%20PET%20Flyer_June18%20PRINT.pdf [consulted: 13-09-2015] | + | <td>[15] </td> |
| + | <td> Yang TH et al. Biosynthesis of polylactic acid and its copolymers using evolved propionate CoA transferase and PHA synthase. (2010) Biotechnol Bioeng 105:150–160</td> |
| + | </tr> |
| | | |
− | <br>[12] The Field Position. The Importance of Corn.2012. < http://www.thefieldposition.com/2012/06/the-importance-of-corn/> [consulted: 13-09-2015] | + | <tr> |
− | <br>[13] Yale iGEM Team 2013. <https://2013.igem.org/Team:Yale> [consulted: 13-09-2015] | + | <td>[16] </td> |
− | <br>[14] Jung et al. Metabolic Engineering of Escherichia coli for the Production of Polylactic Acid and Its Copolymers. (2010) Biotechnology and Bioengineering, Vol. 105, No. 1 | + | <td> Park SJ et al. Mutants of PHA synthase from Pseudomonas sp.6-19 and method for preparing lactate homopolymer or copolymer using the same. (2008b) WO/2008/062999</td> |
− | <br>[15] Yang TH et al. Biosynthesis of polylactic acid and its copolymers using evolved propionate CoA transferase and PHA synthase. (2010) Biotechnol Bioeng 105:150–160
| + | </tr> |
− | <br>[16] Park SJ et al. Mutants of PHA synthase from Pseudomonas sp.6-19 and method for preparing lactate homopolymer or copolymer using the same. (2008b) WO/2008/062999
| + | |
− | <br>[17] Mahishi et al. Poly(3-hydroxybutyrate) (PHB) synthesis by recombinant Escherichia coli harbouring Streptomyces aureofaciens PHB biosynthesis genes: Effect of various carbon and nitrogen sources. Microbiol. Res. (2003) 158, 19–27 | + | |
− | <br>[18] Jacquel N, Lo C-W, Wei Y-H, Wu H-S, Wang SS: Isolation and purification of bacterial poly(3-hydroxyalkanoates). Biochem Eng J 2008, 39:15–27 | + | |
− | <br>[19] Asif Rahman. Secretion of polyhydroxybutyrate in Escherichia coli using a synthetic biological engineering approach. (2013) Journal of Biological Engineering 2013, 7:24
| + | |
− | <br>[20] Maria Enquist-Newman. Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform. (2014) Nature. Vol 505. p239-243
| + | |
− | <br>[21] Wargacki et al. An Engineered Microbial Platform for Direct Biofuel Production from Brown Macroalgae. (2012). Science. Vol 335 p308-313
| + | |
− | <br>[22] Hannover, 2001. The Hannover Principles. Design Guidelines for EXPO 2000. The Word’s Fair
| + | |
− | <br>[23] Dartmouth Undergradruate Journal of Science. Biodegradable Plastic: Its Promises and Consequences. 2013. [online] <http://dujs.dartmouth.edu/applied_sciences/biodegradable-plastic-its-promises-and-consequences#.VfRA2BF_Oko> [consulted: 12-09-2015]
| + | |
| | | |
− | <br>[24] M. Gumel, M. Annuar , Y. Chisti. Recent Advances in the Production, Recovery and Applications of Polyhydroxyalkanoates. (2012) Journal of Polymers & the Environment., Vol. 21. p580 | + | <tr> |
− | <br>[25] Y. Kohara, K. Akiyama, K. Isono. The physical map of the whole E. coli chromosome: Application of a new strategy for rapid analysis and sorting of a large genomic library. (1987) Cell, Vol 50 (3), 495:508 | + | <td>[17] </td> |
− | <br>[26] 8.Jong et al. Production of recombinant proteins by high cell density culture of Escherichia coli. Chemical Engineering Science (2006). Vol. 61, Issue 3, 876–885. | + | <td> Mahishi et al. Poly(3-hydroxybutyrate) (PHB) synthesis by recombinant Escherichia coli harbouring Streptomyces aureofaciens PHB biosynthesis genes: Effect of various carbon and nitrogen sources. Microbiol. Res. (2003) 158, 19–27</td> |
− | <br>[27] AQUA. Analizan potencialidad del cultivo de algas pardas en la Región de Coquimbo. 2007. [online] <http://www.aqua.cl/2007/10/22/analizan-potencialidad-del-cultivo-de-algas-pardas-en-la-region-de-coquimbo/> [consulted: 12-09-2015] | + | </tr> |
− | <br>[28] Subsecretaría de Pesca y Acuicultura. Propuesta Plan de Manejo de la Pesquería de Algas Pardas Región de Arica y Parinacota. [online] <http://www.subpesca.cl/institucional/602/articles-60006_recurso.pdf> [consulted: 12-09-2015] | + | |
− | <br>[29] Ministerio De Economía, Fomento Y Turismo. Informe Tecnico (R. Pesq.) N°74 - 2010 Acoge Medidas Administrativas Para El Recurso Algas Pardas En Zonas De Libre Acceso De Las Regiones De Los Ríos Y Los Lagos Recomendadas Mediante Informe Técnico Dzp N° 005/2010</p> | + | <tr> |
− |
| + | <td>[18] </td> |
− |
| + | <td>Jacquel N, Lo C-W, Wei Y-H, Wu H-S, Wang SS: Isolation and purification of bacterial poly(3-hydroxyalkanoates). Biochem Eng J 2008, 39:15–27 </td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td>[19] </td> |
| + | <td>Asif Rahman. Secretion of polyhydroxybutyrate in Escherichia coli using a synthetic biological engineering approach. (2013) Journal of Biological Engineering 2013, 7:24 </td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td>[20] </td> |
| + | <td> Maria Enquist-Newman. Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform. (2014) Nature. Vol 505. p239-243</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td> [21]</td> |
| + | <td> Wargacki et al. An Engineered Microbial Platform for Direct Biofuel Production from Brown Macroalgae. (2012). Science. Vol 335 p308-313</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td>[22] </td> |
| + | <td> Hannover, 2001. The Hannover Principles. Design Guidelines for EXPO 2000. The Word’s Fair></td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td>[23] </td> |
| + | <td> Dartmouth Undergradruate Journal of Science. Biodegradable Plastic: Its Promises and Consequences. 2013. [online] <http://dujs.dartmouth.edu/applied_sciences/biodegradable-plastic-its-promises-and-consequences#.VfRA2BF_Oko> [consulted: 12-09-2015]</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td>[24] </td> |
| + | <td> M. Gumel, M. Annuar , Y. Chisti. Recent Advances in the Production, Recovery and Applications of Polyhydroxyalkanoates. (2012) Journal of Polymers & the Environment., Vol. 21. p580</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td>[25] </td> |
| + | <td> Y. Kohara, K. Akiyama, K. Isono. The physical map of the whole E. coli chromosome: Application of a new strategy for rapid analysis and sorting of a large genomic library. (1987) Cell, Vol 50 (3), 495:508</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td>[26] </td> |
| + | <td> 8.Jong et al. Production of recombinant proteins by high cell density culture of Escherichia coli. Chemical Engineering Science (2006). Vol. 61, Issue 3, 876–885.</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td>[27] </td> |
| + | <td> AQUA. Analizan potencialidad del cultivo de algas pardas en la Región de Coquimbo. 2007. [online] <http://www.aqua.cl/2007/10/22/analizan-potencialidad-del-cultivo-de-algas-pardas-en-la-region-de-coquimbo/> [consulted: 12-09-2015]</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td>[28] </td> |
| + | <td> Subsecretaría de Pesca y Acuicultura. Propuesta Plan de Manejo de la Pesquería de Algas Pardas Región de Arica y Parinacota. [online] <http://www.subpesca.cl/institucional/602/articles-60006_recurso.pdf> [consulted: 12-09-2015]</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td>[29]</td> |
| + | <td> Ministerio De Economía, Fomento Y Turismo. Informe Tecnico (R. Pesq.) N°74 - 2010 Acoge Medidas Administrativas Para El Recurso Algas Pardas En Zonas De Libre Acceso De Las Regiones De Los Ríos Y Los Lagos Recomendadas Mediante Informe Técnico Dzp N° 005/2010</td> |
| + | </tr> |
| + | |
| + | </table> |
| + | |
| + | |
| </div> | | </div> |
| </article> | | </article> |
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| <h1>How does our PLAdora system work?</h1> | | <h1>How does our PLAdora system work?</h1> |
| <!-------COLUMNA 1--> | | <!-------COLUMNA 1--> |
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| <p>When HSL molecules diffuse from bacteria 1 to bacteria 2, will bind LuxR protein (completing quorum sensing) and will induced plux promoter so pCoAT and phaC enzyme and phasin-HIyA protein will be expressed at the same time. This system depend of the first system, because if pH is lower than 5.5 (e.g. due to a low consumption of lactate from medium by the second system), there is no production either of lactate and HSL in the first system so there is no induction of plux promoter by LuxR-HSL complex, thereby second system would be OFF.</p> | | <p>When HSL molecules diffuse from bacteria 1 to bacteria 2, will bind LuxR protein (completing quorum sensing) and will induced plux promoter so pCoAT and phaC enzyme and phasin-HIyA protein will be expressed at the same time. This system depend of the first system, because if pH is lower than 5.5 (e.g. due to a low consumption of lactate from medium by the second system), there is no production either of lactate and HSL in the first system so there is no induction of plux promoter by LuxR-HSL complex, thereby second system would be OFF.</p> |
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− | <img id="Esquema" width=150 alt="Bacteria SuperHeroe" src="https://static.igem.org/mediawiki/2015/c/ca/UChile-Openbio-DES_PLAdora1_pag4.png" /><br>
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− | <img id="Esquema" class="middle top" width=150 alt="Bacteria SuperHeroe" src="https://static.igem.org/mediawiki/2015/6/68/UChile-Openbio-DES_PLAdora2_pag4.png" /><br>
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− | <img id="Esquema" class="middle top" width=150 alt="Bacteria SuperHeroe" src="https://static.igem.org/mediawiki/2015/1/1a/UChile-Openbio-DES_PLAdora4_pag4.png" /><br>
| + | <td><img id="Esquema" class="middle top" width=350 alt="Bacteria SuperHeroe" src="https://static.igem.org/mediawiki/2015/c/ca/UChile-Openbio-DES_PLAdora1_pag4.png" /></td> |
− | </div>
| + | <td><img id="Esquema" class="middle top" width=350alt="Bacteria SuperHeroe" src="https://static.igem.org/mediawiki/2015/6/68/UChile-Openbio-DES_PLAdora2_pag4.png" /></td> |
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− | <img id="Esquema" class="middle top" width=150 alt="Bacteria SuperHeroe" src="https://static.igem.org/mediawiki/2015/7/78/UChile-Openbio-DES_PLAdora3_pag4.png" /><br>
| + | <td><img id="Esquema" class="middle top" width=350alt="Bacteria SuperHeroe" src="https://static.igem.org/mediawiki/2015/7/78/UChile-Openbio-DES_PLAdora3_pag4.png" /></td> |
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| <h2 ALIGN="center">Represantations of interaction of the different modules of the second system</h2> | | <h2 ALIGN="center">Represantations of interaction of the different modules of the second system</h2> |
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| </article> | | </article> |
| </section> | | </section> |
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| <img id="Esquema" class="middle top" alt="Modules" src="https://static.igem.org/mediawiki/2015/e/e9/UChile-Openbio-DES_Safety_pag6.png" /> | | <img id="Esquema" class="middle top" alt="Modules" src="https://static.igem.org/mediawiki/2015/e/e9/UChile-Openbio-DES_Safety_pag6.png" /> |
| + | <br> |
| </div> | | </div> |
| </div> | | </div> |
| </article> | | </article> |
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| </section> | | </section> |
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