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| − | {{Template_All_Teams}}
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| − | <html>
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| − | </div></div></div></div></div></div></div></div></div></div>
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| − | </html>
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| − | {{Team:UMarylandClasses}}
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| − | {{Team:UMarylandMenu}}
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| − | <html>
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| | | | |
| − | <!--Attention! If you are not part of the website team, you are NOT allowed to touch anything above this line without the express permission of Best Kohai.-->
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| − |
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| − | <style type="text/css">
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| − |
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| − | h1,h2,h3,h4,p,a {
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| − | text-decoration: none;
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| − | }
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| − | h1 {
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| − | font-size: 250%;
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| − | }
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| − | h2 {
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| − | font-size: 200%;
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| − | }
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| − | h3 {
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| − | font-size: 150%;
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| − | }
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| − | h4 {
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| − | font-size: 125%;
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| − | }
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| − | p {
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| − | font-size: 100%;
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| − | }
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| − | #sidemenu {
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| − | position:fixed;
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| − | float:left;
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| − | width: 200px;
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| − | top:200px;
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| − | }
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| − |
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| − | #sidemenu,
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| − | #sidemenu ul,
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| − | #sidemenu ul li,
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| − | #sidemenu ul li a {
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| − | margin: 0;
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| − | padding: 0;
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| − | border: 0;
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| − | list-style: none;
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| − | line-height: 1;
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| − | display: block;
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| − | -webkit-box-sizing: border-box;
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| − | -moz-box-sizing: border-box;
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| − | box-sizing: border-box;
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| − | }
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| − |
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| − | #sidemenu ul ul {
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| − | display: none;
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| − | }
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| − | .align-right {
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| − | float: right;
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| − | }
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| − | #sidemenu > ul > li > a {
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| − | padding: 15px 20px;
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| − | border: 1px solid black;
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| − | cursor: pointer;
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| − | z-index: 2;
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| − | font-size: 14px;
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| − | font-weight: bold;
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| − | text-decoration: none;
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| − | color: #000000;
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| − | background-image: -webkit-linear-gradient(bottom, #ffffff 0%, #2BB1FF 100%);
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| − | box-shadow: inset 0 1px 0 rgba(255, 255, 255, 0.15);
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| − | }
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| − |
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| − | #sidemenu > ul > li > a:hover,
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| − | #sidemenu > ul > li.active > a,
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| − | #sidemenu > ul > li.open > a {
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| − | color: #000000;
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| − | background-image: -webkit-linear-gradient(top, #C9C9C9 0%, #FFFFFF 100%);
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| − | }
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| − | #sidemenu > ul > li.open > a {
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| − | box-shadow: inset 0 1px 0 rgba(255, 255, 255, 0.15), 0 1px 1px rgba(0, 0, 0, 0.15);
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| − | border-bottom: 1px solid #1682ba;
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| − | }
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| − |
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| − |
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| − | #sidemenu > ul > li > a:hover > span::after,
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| − | #sidemenu > ul > li.active > a > span::after,
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| − | #sidemenu > ul > li.open > a > span::after {
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| − | border-color: #eeeeee;
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| − | }
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| − | .holder::before {
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| − | top: 18px;
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| − | border-top: 2px solid;
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| − | border-left: 2px solid;
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| − | border-top-color: inherit;
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| − | border-left-color: inherit;
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| − | }
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| − | #sidemenu ul ul li a {
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| − | cursor: pointer;
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| − | border-bottom: 1px solid #32373e;
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| − | border-left: 1px solid #32373e;
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| − | border-right: 1px solid #32373e;
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| − | padding: 10px 20px;
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| − | z-index: 1;
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| − | text-decoration: none;
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| − | font-size: 13px;
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| − | color: #eeeeee;
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| − | background: #49505a;
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| − | box-shadow: inset 0 1px 0 rgba(255, 255, 255, 0.1);
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| − | }
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| − | #sidemenu ul ul li:hover > a,
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| − | #sidemenu ul ul li.open > a,
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| − | #sidemenu ul ul li.active > a {
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| − | background: #424852;
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| − | color: #ffffff;
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| − | }
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| − | #sidemenu ul ul li:first-child > a {
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| − | box-shadow: none;
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| − | }
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| − | #sidemenu ul ul ul li:first-child > a {
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| − | box-shadow: inset 0 1px 0 rgba(255, 255, 255, 0.1);
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| − | }
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| − | #sidemenu ul ul ul li a {
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| − | padding-left: 30px;
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| − | }
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| − | #sidemenu > ul > li > ul > li:last-child > a,
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| − | #sidemenu > ul > li > ul > li.last > a {
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| − | border-bottom: 0;
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| − | }
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| − | #sidemenu > ul > li > ul > li.open:last-child > a,
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| − | #sidemenu > ul > li > ul > li.last.open > a {
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| − | border-bottom: 1px solid #32373e;
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| − | }
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| − | #sidemenu > ul > li > ul > li.open:last-child > ul > li:last-child > a {
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| − | border-bottom: 0;
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| − | }
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| − | #sidemenu ul ul li.has-sub > a::after {
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| − | display: block;
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| − | position: absolute;
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| − | content: "";
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| − | width: 5px;
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| − | height: 5px;
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| − | right: 20px;
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| − | z-index: 10;
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| − | top: 11.5px;
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| − | border-top: 2px solid #eeeeee;
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| − | border-left: 2px solid #eeeeee;
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| − | -webkit-transform: rotate(-135deg);
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| − | -moz-transform: rotate(-135deg);
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| − | -ms-transform: rotate(-135deg);
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| − | -o-transform: rotate(-135deg);
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| − | transform: rotate(-135deg);
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| − | }
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| − | #sidemenu ul ul li.active > a::after,
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| − | #sidemenu ul ul li.open > a::after,
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| − | #sidemenu ul ul li > a:hover::after {
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| − | border-color: #ffffff;
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| − | }
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| − |
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| − | #layer1 {
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| − | overflow:hidden;
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| − | position:relative;
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| − | width:100%;
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| − | margin:0px;
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| − | padding:20px;
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| − | background-color: #89e1ff;
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| − | min-height:42px;
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| − | top:-245px;
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| − | border-top:2px solid black;
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| − | }
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| − | #layer2 {
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| − | overflow:hidden;
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| − | position:relative;
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| − | width:100%;
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| − | margin:0px;
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| − | padding:20px;
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| − | background-color:#ffffff;
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| − | min-height:42px;
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| − | top:-245px;
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| − | border-top:2px solid black;
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| − | }
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| − | #layer3 {
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| − | overflow:hidden;
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| − | position:relative;
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| − | width:100%;
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| − | margin:0px;
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| − | padding:20px;
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| − | background-color:#7fcff9;
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| − | min-height:42px;
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| − | top:-245px;
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| − | border-top:2px solid black;
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| − | }
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| − | #layer4 {
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| − | overflow:hidden;
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| − | position:relative;
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| − | width:100%;
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| − | margin:0px;
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| − | padding:20px;
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| − | background-color:#ffffff;
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| − | min-height:42px;
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| − | }
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| − | #contentbox{
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| − | width: 80%;
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| − | max-width: 1900px;
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| − | min-width: 1000px;
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| − | text-align: justify;
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| − | margin:auto;
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| − | font-family: "Palatino Linotype", "Book Antiqua", Palatino, serif;
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| − | }
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| − | #cover {
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| − | overflow:hidden;
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| − | position:relative;
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| − | width:100%;
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| − | margin:auto;
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| − | padding:0px;
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| − | background-image: url("https://static.igem.org/mediawiki/2015/9/97/Resultsq.png");
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| − | background-size: 100% ;
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| − | background-repeat: no-repeat;
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| − | height: 100%;
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| − | width:100%;
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| − | min-height:800px;
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| − | color: #0DFF00;
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| − | font-family: Verdana, Geneva, sans-serif;
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| − | font-size:xx-large;
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| − | top:-200px;
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| − | }
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| − | #buttonset {
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| − | text-align:center;
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| − | bottom:0px;
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| − | position:relative;
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| − | top:500px;
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| − | }
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| − | #button {
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| − | overflow:hidden;
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| − | display: inline-block;
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| − | height:50px;
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| − | width: 100px;
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| − | background-color: rgba(255,255,255,.5);
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| − | border: 2px solid black;
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| − | border-radius: 10px;
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| − | margin: 10px;
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| − | padding:10px;
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| − | font-size:medium;
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| − | color:black;
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| − | text-align: center;
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| − | }
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| − |
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| − | #bar{
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| − | display: inline-block;
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| − | opacity:1;
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| − | background-color: rgba(255,255,255,.5);
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| − | color:black;
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| − | border-top:4px solid black;
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| − | border-bottom:4px solid black;
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| − | width:100%;
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| − | text-align:center;
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| − | height:200px;
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| − | position:absolute;
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| − | top:200px;
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| − | text-shadow: 4px 4px 4px white;
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| − | }
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| − | </style>
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| − |
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| − | <div id='cover'>
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| − | <div id='bar'>
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| − | <p style="font-size:64px"><b>Notebook</b>
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| − | </div>
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| − | <div id='sidemenu'>
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| − | <ul>
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| − | <li><a href="week1" class="smoothScroll"><span>Week 1</span></a></li>
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| − | <li><a href="week2" class="smoothScroll"><span>week 2</span></a></li>
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| − | <li><a href='week3' class="smoothScroll"><span>week3</span></a></li>
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| − | <li><a href='#grind' class="smoothScroll"><span>The Grind</span></a></li>
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| − | <li><a href='#management' class="smoothScroll"><span>Effective Management</span></a></li>
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| − | <li><a href='#misc' class="smoothScroll"><span>Misc. Advice</span></a></li>
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| − | </ul>
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| − | </div>
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| − | <div id='buttonset'>
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| − | <a href="#Week1">
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| − | <div id='button'>
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| − | <h4>Week1</h4>
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| − | </div>
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| − | </a>
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| − |
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| − | <a href="#Week2">
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| − | <div id='button'>
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| − | <h4>Week2</h4>
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| − | </div>
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| − | </a>
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| − |
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| − | <a href="#Week3">
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| − | <div id='button'>
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| − | <h4>Week3</h4>
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| − | </div>
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| − | </a>
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| − |
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| − | <a href="#Week4">
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| − | <div id='button'>
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| − | <h4>Week4</h4>
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| − | </div>
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| − | </a>
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| − |
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| − | <a href="#Week5">
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| − | <div id='button'>
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| − | <h4>Week5</h4>
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| − | </div>
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| − | </a>
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| − |
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| − | <a href="#Week6">
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| − | <div id='button'>
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| − | <h4>Week6</h4>
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| − | </div>
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| − | </a>
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| − |
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| − |
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| − | <a href="#Week7">
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| − | <div id='button'>
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| − | <h4>Week7</h4>
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| − | </div>
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| − | </a>
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| − |
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| − | <a href="#Week8">
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| − | <div id='button'>
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| − | <h4>Week8</h4>
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| − | </div>
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| − | </a>
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| − |
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| − | <a href="#Week9">
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| − | <div id='button'>
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| − | <h4>Week9</h4>
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| − | </div>
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| − | </a>
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| − |
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| − | <a href="#Week10">
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| − | <div id='button'>
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| − | <h4>Week10</h4>
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| − | </div>
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| − | </a>
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| − |
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| − | <a href="#Week2">
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| − | <div id='button'>
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| − | <h4>Week2</h4>
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| − | </div>
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| − | </a>
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| − |
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| − | <a href="#Week11">
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| − | <div id='button'>
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| − | <h4>Week2</h4>
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| − | </div>
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| − | </a>
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| − |
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| − | <a href="#Week12">
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| − | <div id='button'>
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| − | <h4>Week12</h4>
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| − | </div>
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| − | </a>
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| − |
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| − | <a href="#Week13">
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| − | <div id='button'>
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| − | <h4>Week13</h4>
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| − | </div>
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| − | </a>
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| − | <a href="#Week14">
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| − | <div id='button'>
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| − | <h4>Week14</h4>
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| − | </div>
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| − | </a><a href="#Week15">
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| − | <div id='button'>
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| − | <h4>Week15</h4>
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| − | </div>
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| − | </a>
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| − | <a href="#Week16">
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| − | <div id='button'>
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| − | <h4>Week16</h4>
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| − | </div>
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| − | </a>
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| − |
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| − |
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − |
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| − |
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| − | <div id='layer2'>
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| − | <div id='contentbox'>
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| − | <a name="Week1">
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| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
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| − | <b>Week1</b></a>
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| − | <p style="font-size:24px;text-align:center;">
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| − | Miraculin:
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| − | ligated the pBAD promoter in PSB1C3 with the miraculin gene in PSB1C3 using 3A assembly
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| − | plate had colonies
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| − | <br>
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| − | <br>
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| − | </div>
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| − | </div>
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| − |
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| − | <div id='layer1'>
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| − | <div id='contentbox'>
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| − | <a name="Week2">
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| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
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| − | <b>Week2</b></a>
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| − | <p style="font-size:24px;text-align:center;">
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| − | Miraculin:
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| − | pBAD +miraculin construct was moved back into psb1C3 backbone and sent for sequencing
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| − | Designing gBlocks:
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| − | Designed gblocks for epsilon-cyclase (from arabidopsis), pyocin and Hok/Sok
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| − | <br>
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| − | <br>
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| − | </div>
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| − | </div>
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| − |
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| − | <div id='layer2'>
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| − | <div id='contentbox'>
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| − | <a name="Week3">
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| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
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| − | <b>Week3</b></a>
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| − | <p style="font-size:24px;text-align:center;">
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| − | Miraculin:
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| − | sequence confirmed through sequencing
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| − | failed to extract using French press, FPLC and SDS-Page
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| − | could be due to high arabinose induction (OD of 1)
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| − |
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| − | <br>
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| − | <br>
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| − | </div>
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| − | </div>
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| − | <div id='layer1'>
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| − | <div id='contentbox'>
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| − | <a name="Week4">
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| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
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| − | <b>Week4</b></a>
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| − | <p style="font-size:24px;text-align:center;">
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| − | Miraculin:
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| − | induced 3 test cultures with 0%, 0.05%, 0.1%, 0.2% arabinose with no significant results
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| − | will perform procedure again using bl21 instead of Dh5a
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| − |
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| − |
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| − | <br>
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| − | <br>
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| − | </div>
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| − | </div>
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| − |
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| − | <div id='layer2'>
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| − | <div id='contentbox'>
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| − | <a name="Week5">
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| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
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| − | <b>Week5</b></a>
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| − | <p style="font-size:24px;text-align:center;">
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| − | Miraculin
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| − | transformation in BL21 strain was successful
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| − | SDS page showed a band at roughly 25 kDa
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| − | Hok/Sok
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| − | inserted Hok/Sok gblock into PSB1C3 backbone using Gibson assembly
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| − | construct sent for sequencing
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| − | Interlab Study
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| − | transformed parts into dh5a: 3 Anderson promoters, GFP, and RFP
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| − |
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| − |
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| − | <br>
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| − | <br>
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| − | </div>
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| − | </div>
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| − | <div id='layer1'>
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| − | <div id='contentbox'>
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| − | <a name="Week4">
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| − | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">
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| − | <b>Week4</b></a>
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| − | <p style="font-size:24px;text-align:center;">
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| − | Miraculin:
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| − | induced 3 test cultures with 0%, 0.05%, 0.1%, 0.2% arabinose with no significant results
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| − | will perform procedure again using bl21 instead of Dh5a
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| − |
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| − |
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| − | <br>
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| − | <br>
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| − | </div>
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| − | </div>
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| − | </div>
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| − |
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| − | </html>
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