Difference between revisions of "Team:UMass-Dartmouth/ourproject"
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<div id="sub"> | <div id="sub"> | ||
| − | <p id="title"> | + | <p id="title"><br><br> |
OBJECTIVE: | OBJECTIVE: | ||
</p> | </p> | ||
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<div id="section1"> | <div id="section1"> | ||
<div id="sub1"> | <div id="sub1"> | ||
| − | <p id="title"> | + | <p id="title"><br><br> |
PROJECT PROPOSAL: | PROJECT PROPOSAL: | ||
</p> | </p> | ||
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| − | References | + | References<br> |
| − | 1. Scott Algood H, Cover T. “Helicobacter pylori Persistence: an Overview of Interactions between H. pylori and Host Immune Defenses.” Clinical Microbial Review. 2006; 19(4): 597-613. | + | 1. Scott Algood H, Cover T. “Helicobacter pylori Persistence: an Overview of Interactions between H. pylori and Host Immune Defenses.” Clinical Microbial Review. 2006; 19(4): 597-613. <br> |
| − | 2. Zou Q, Wei W. “Phage Therapy: Promising For H. pylori Infection.” Clinical Microbiology. 2013; 2: 112. | + | 2. Zou Q, Wei W. “Phage Therapy: Promising For H. pylori Infection.” Clinical Microbiology. 2013; 2: 112. <br> |
| − | 3. Blanke S, Ye D. Alternative Mechanisms of Protein Release. In: Mobley H, Mendz G, Hazell S, editors. Helicobacter pylori: Physiology and Genetics Washington DC: ASM Press; 2001. Chapter 20. | + | 3. Blanke S, Ye D. Alternative Mechanisms of Protein Release. In: Mobley H, Mendz G, Hazell S, editors. Helicobacter pylori: Physiology and Genetics Washington DC: ASM Press; 2001. Chapter 20.<br> |
| − | 4. Houimel M, Mach J, Corthesy-Theulaz I, Corthesy B, Fisch I. New inhibitors of Helicobacter pylori urease holoenzyme selected from phage-displayed peptide libraries. Eur J Biochem. 1999; 262:774-780. | + | 4. Houimel M, Mach J, Corthesy-Theulaz I, Corthesy B, Fisch I. New inhibitors of Helicobacter pylori urease holoenzyme selected from phage-displayed peptide libraries. Eur J Biochem. 1999; 262:774-780.<br> |
5. Pflock M, Kennard S, Delany I, Scarlato V, Beier D. Acid-Induced Activation of the Urease Promoters Is Mediated Directly by the ArsRS Two-Component System of Helicobacter pylori. Infect Immun. 2005; 73(10): 6437-6445. | 5. Pflock M, Kennard S, Delany I, Scarlato V, Beier D. Acid-Induced Activation of the Urease Promoters Is Mediated Directly by the ArsRS Two-Component System of Helicobacter pylori. Infect Immun. 2005; 73(10): 6437-6445. | ||
<br> | <br> | ||
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<div id="section"> | <div id="section"> | ||
<div id="sub2"> | <div id="sub2"> | ||
| − | <p id="title"> | + | <p id="title"><br><br> |
PARTS: | PARTS: | ||
</p> | </p> | ||
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Individual components<br> | Individual components<br> | ||
| − | Prefix (with start codon): | + | Prefix (with start codon): <p style="color:gold">GTTTCTTCGAATTCGCGGCCGCTTCTAG</p> |
| − | + | <p style="color:green">RBS | |
| − | + | BBa_B0030 : ATTAAAGAGGAGAAA</p> | |
| − | BBa_B0030 : ATTAAAGAGGAGAAA < | + | <p style="color:purple">torA <br> |
| − | + | ||
BBa_K1012002: <br> | BBa_K1012002: <br> | ||
ATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTGGCCGGGA<br> | ATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTGGCCGGGA<br> | ||
| − | TGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCG< | + | TGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCG</p> |
| − | 6-mer: TATGATTTTTATTGGTGG < | + | <p style="color:skyblue">6-mer: TATGATTTTTATTGGTGG</p> |
| − | Suffix (Double stop codon): | + | <p style="color:gold">Suffix (Double stop codon): |
| − | TAATAATACTAGTAGCGGCCGCTGCAGGAAGAAAC | + | TAATAATACTAGTAGCGGCCGCTGCAGGAAGAAAC </p><br> |
| − | KTP6merNG3 – Composite part | + | <strong style="color:black">KTP6merNG3 – Composite part</strong> |
| − | + | <p style="color:gold"><p style="gold">GTTTCTTCGAATTCGCGGCCGCTTCTAG</p>-<p style="color:green">ATTAAAGAGGAGAAA</p> | |
| + | ATGAACAATAACGATCTCTTTCAGGCATCACGT | ||
CGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTGGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACG<br> | CGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTGGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACG<br> | ||
TGCGACTGCGGCGCAAGCGTATGATTTTTATTGGTGGTAATAATACTAGTAGCGGCCGCTGCAGGAAGAAAC<br><br> | TGCGACTGCGGCGCAAGCGTATGATTTTTATTGGTGGTAATAATACTAGTAGCGGCCGCTGCAGGAAGAAAC<br><br> | ||
Revision as of 04:15, 18 September 2015
