Difference between revisions of "Team:WPI-Worcester"

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{{WPI-Worcester}}
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<html>
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<head>
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<h2> Welcome to iGEM 2015! </h2>
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<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<link rel="alternate" type="application/atom+xml" title="2014.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&amp;feed=atom" /> <title>Team:WPI-Worcester - 2014.igem.org</title>
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<h4>Before you start: </h4>
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<p> Please read the following pages:</p>
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  <div id="globalWrapper">
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<ul>
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<li>  <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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</ul>
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<div class="highlightBox">
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    <div id="top-section">
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<h4> Styling your wiki </h4>
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<div id="p-logo">
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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    <a href="/Main_Page"
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<script type="text/javascript"> if (window.isMSIE55) fixalpha(); </script>
  
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<h4> Editing your wiki </h4>
 
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
 
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<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:WPI-Worcester&action=edit"> Click here to edit this page! </a></p>
 
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<p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>
 
  
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    <div id="content">
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<a name="top" id="top"></a>
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<h1 class="firstHeading">Team:WPI-Worcester</h1>
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<h3 id="siteSub" class='noprint'>From 2014.igem.org</h3>
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<p>
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<style>
  
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<h4>Templates </h4>
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a{
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<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
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color:#AC2B37;
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<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>
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 +
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−
<h4>Tips</h4>
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a:visited{
−
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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color:#AC2B37;
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<ul>
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}
−
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
−
<li>Be clear about what you are doing and how you plan to do this.</li>
+
−
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
−
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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−
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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<li>Have lots of fun! </li>
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−
</ul>
+
  
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    padding-right: 10px;
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−
<h4>Inspiration</h4>
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tr {
−
<p> You can also view other team wikis for inspiration! Here are some examples:</p>
+
    vertical-align: top;
−
<ul>
+
}
−
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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−
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
+
−
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
+
−
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
+
−
</ul>
+
  
−
<h4> Uploading pictures and files </h4>
+
H3 {
−
<p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
+
      font-family: Georgia;
−
When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
+
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−
<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
+
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<body class="basiclayout">
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            <li><a href="https://2014.igem.org/Team:WPI-Worcester/Motivation">Motivation</a></li>
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            <li><a href="https://2014.igem.org/Team:WPI-Worcester/Beyond-the-Bench">Beyond the Bench</a></li>
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<li><a href="#"><center><img src="https://static.igem.org/mediawiki/2014/e/e9/Acknowledgements_Link.png"/></center><p>Acknowledgements</p></a>
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<li><a href="https://2014.igem.org/Team:WPI-Worcester/Sponsors">Sponsors</a></li>
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<li><a href="https://2014.igem.org/Team:WPI-Worcester/Attributions">Attributions</a></li>
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</div>
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</body>
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<h4>Building a Library of Antifreeze Proteins:</h4><p>Antifreeze proteins (AFPs) are a structurally diverse class of proteins that alter the dynamics of ice crystallization and thereby permit organisms to survive in temperatures below the freezing point of water. AFPs have tremendous promise in biotechnology applications including cryogenic tissue preservation, freeze tolerance in crops, and improvement in the quality of frozen foods. AFPs have been utilized in a limited way by a number of previous iGEM teams, though very few AFPs have been analyzed thoroughly for use in E. coli, and rarely have they been explored comparatively. One of the most exciting new applications for APFs has only recently been discovered: Heisig et al. (2014) showed that a tick antifreeze glycoprotein, IAFGP, was disruptive to the formation of biofilms--cooperative structures formed by bacteria that contribute to serious problems in manufacturing, food processing, and human health. It remains unknown whether this biofilm-inhibiting property is a common feature of APFs or is specific to AFPs with particular structures or sequences. </p>
 +
 
 +
<p>Our aim is to create a library of antifreeze proteins collected from diverse kingdoms of life and, by engineering E. coli to express them, test their antifreeze and biofilm-disruption properties. In addition, we hope to determine whether expressing multiple antifreeze proteins confers a cooperative advantage over single proteins. Finally, we plan to use the information learned to design synthetic antifreeze proteins for E. coli, idealized for either surviving low temperatures or disrupting biofilms. Our project will create a new toolkit for the synthetic biology community that will facilitate the development of synthetic systems that must survive in subzero temperatures, in addition to providing a potentially novel method for the control of biofilm dynamics.
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Revision as of 16:19, 15 July 2015

Team:WPI-Worcester - 2014.igem.org

 

Team:WPI-Worcester

From 2014.igem.org


Building a Library of Antifreeze Proteins:

Antifreeze proteins (AFPs) are a structurally diverse class of proteins that alter the dynamics of ice crystallization and thereby permit organisms to survive in temperatures below the freezing point of water. AFPs have tremendous promise in biotechnology applications including cryogenic tissue preservation, freeze tolerance in crops, and improvement in the quality of frozen foods. AFPs have been utilized in a limited way by a number of previous iGEM teams, though very few AFPs have been analyzed thoroughly for use in E. coli, and rarely have they been explored comparatively. One of the most exciting new applications for APFs has only recently been discovered: Heisig et al. (2014) showed that a tick antifreeze glycoprotein, IAFGP, was disruptive to the formation of biofilms--cooperative structures formed by bacteria that contribute to serious problems in manufacturing, food processing, and human health. It remains unknown whether this biofilm-inhibiting property is a common feature of APFs or is specific to AFPs with particular structures or sequences.

Our aim is to create a library of antifreeze proteins collected from diverse kingdoms of life and, by engineering E. coli to express them, test their antifreeze and biofilm-disruption properties. In addition, we hope to determine whether expressing multiple antifreeze proteins confers a cooperative advantage over single proteins. Finally, we plan to use the information learned to design synthetic antifreeze proteins for E. coli, idealized for either surviving low temperatures or disrupting biofilms. Our project will create a new toolkit for the synthetic biology community that will facilitate the development of synthetic systems that must survive in subzero temperatures, in addition to providing a potentially novel method for the control of biofilm dynamics.