Difference between revisions of "Team:WashU StLouis"
Ayekedavid (Talk | contribs) |
|||
(2 intermediate revisions by 2 users not shown) | |||
Line 1: | Line 1: | ||
{{:Team:WashU_StLouis/Header}} | {{:Team:WashU_StLouis/Header}} | ||
<html> | <html> | ||
− | + | <!-- Navigation --> | |
− | + | ||
<nav class="navbar navbar-default navbar-fixed-top"> | <nav class="navbar navbar-default navbar-fixed-top"> | ||
<div class="container"> | <div class="container"> | ||
Line 57: | Line 56: | ||
<div class="container"> | <div class="container"> | ||
+ | <div class="col-lg-12 text-center"> | ||
+ | <h3 class="section-subheading text-muted"><a class="page-scroll" href="https://static.igem.org/mediawiki/2015/d/d2/2016_WashU_iGEM_Application.pdf">Click here to apply for the 2016 iGEM Team!</a></h3> | ||
+ | </div> | ||
Line 1,023: | Line 1,025: | ||
</p> | </p> | ||
<p dir="ltr"> | <p dir="ltr"> | ||
− | - | + | -Take the bottle to an autoclave |
</p> | </p> | ||
<p dir="ltr"> | <p dir="ltr"> | ||
− | - | + | -Autoclave bottle on agar cycle, loose cap, for two hours |
</p> | </p> | ||
<br/> | <br/> | ||
Line 1,073: | Line 1,075: | ||
</p> | </p> | ||
<p dir="ltr"> | <p dir="ltr"> | ||
− | (NOTE: | + | (NOTE: % glycerol in each bottle is roughly 4.4%) |
</p> | </p> | ||
<p dir="ltr"> | <p dir="ltr"> | ||
− | -Bottles were placed back into centrifuge for another | + | -Bottles were placed back into centrifuge for another 25 minutes at 4 deg C |
</p> | </p> | ||
<p dir="ltr"> | <p dir="ltr"> | ||
Line 1,111: | Line 1,113: | ||
<br/> | <br/> | ||
<p dir="ltr"> | <p dir="ltr"> | ||
− | - | + | -Obtain plasmid DNA |
</p> | </p> | ||
<p dir="ltr"> | <p dir="ltr"> | ||
Line 1,120: | Line 1,122: | ||
</p> | </p> | ||
<p dir="ltr"> | <p dir="ltr"> | ||
− | - | + | -Pipet entirety of electrocompetent cell mixture into electro cuvette |
</p> | </p> | ||
<p dir="ltr"> | <p dir="ltr"> | ||
− | - | + | -Shock cell/DNA mixture using an Eporator |
</p> | </p> | ||
<p dir="ltr"> | <p dir="ltr"> | ||
− | - | + | -Take out strain and pour into 100 uL of LB to be grown for 1 hour |
</p> | </p> | ||
<p dir="ltr"> | <p dir="ltr"> | ||
− | -After 1 hour of growth, strain | + | -After 1 hour of growth, plate strain onto LB/Agar/Antibiotic |
</p> | </p> | ||
<br/> | <br/> | ||
Line 1,137: | Line 1,139: | ||
<br/> | <br/> | ||
<p dir="ltr"> | <p dir="ltr"> | ||
− | - | + | -Mix 500 uL of cell culture with 500 uL of 30% glycerol |
</p> | </p> | ||
<p dir="ltr"> | <p dir="ltr"> | ||
− | - | + | -Store in -80 deg C freezer |
</p> | </p> | ||
<br/> | <br/> | ||
Line 1,528: | Line 1,530: | ||
<p class="muted">XKCD</p> | <p class="muted">XKCD</p> | ||
</a> | </a> | ||
− | + | <p>The overarching goal behind our project is to introduce nitrogen fixation within plants. Through the introduction of this technology, the need for fertilizers would be greatly diminished, which would provide many positive environmental impacts and cut costs.</p> | |
+ | <p>Most of our fertilizer today is produced through the Haber Process. The Haber Process is widely believed to have triggered a population explosion, fueling the growth of human population around the world from 1.6 million to 7 million.<a href="#" data-container="body" data-toggle="popover" data-placement="top" data-content="Smil, Vaclav Detonator of the population explosion" ><sup>[1]</sup></a> However, there is evidence for detrimental effects surrounding the use of nitrogen compounds. Near areas of heavy industrialization, the development of dead zones have been observed.<a href="#" data-container="body" data-toggle="popover" data-placement="top" data-content="Howarth, Robert Coastal nitrogen pollution: A review of sources and trends globally and regionally" ><sup>[2]</sup></a> Eutrophication results in the development of harmful algal blooms, triggering hypoxia and hurting the delicate balance of aquatic ecosystems. </p> | ||
+ | <p>Through our project, we hope to undercut the production of fertilizer and reduce its harmful environmental impact. Through having plants synthesize their own nitrogen compounds we eliminate the need for that artificial nitrogen source. By working in E. coli, it is the team’s hope to develop tools for experimentation with nitrogen fixation in other organisms. E. coli holds the distinct advantage of having rapid growth cycles, enabling quick experimentation with different biological technologies. </p> | ||
+ | <h4>References</h4> | ||
+ | <ol> | ||
+ | <li><a href="http://www.nature.com/nature/journal/v400/n6743/full/400415a0.html">http://www.nature.com/nature/journal/v400/n6743/full/400415a0.html</a></li> | ||
+ | <li><a href="http://www.sciencedirect.com/science/article/pii/S1568988308001078">http://www.sciencedirect.com/science/article/pii/S1568988308001078</a></li> | ||
+ | </ol> | ||
<button type="button" class="btn btn-primary" data-dismiss="modal"><span class="icon-remove"></span> Close Modal</button> | <button type="button" class="btn btn-primary" data-dismiss="modal"><span class="icon-remove"></span> Close Modal</button> | ||
</div> | </div> | ||
Line 1,536: | Line 1,545: | ||
</div> | </div> | ||
</div> | </div> | ||
− | |||
− | |||
− | |||
</html> | </html> | ||
{{:Team:WashU_StLouis/Footer}} | {{:Team:WashU_StLouis/Footer}} |
Latest revision as of 15:57, 27 October 2015
Team
NitroGeniuses
-
Jessica O'Callaghan
Chemical Engineering
Penn State
-
Laura Beebe
Biochemistry and Molecular Biology
Penn State
-
Blake Actkinson
Biomedical Engineering
Washington University in St. Louis
-
Charlotte Bourg
Biochemistry and Spanish
Washington University in St. Louis
-
David Ayeke
Computer Engineering
Washington University in St. Louis
-
Mike Toomey
Biomedical Engineering
Washington University in St. Louis
-
Caroline Focht
- 2014 iGEM Team MemberChemistry and Biochemistry
Washington University in St. Louis
Collaboration
Looking for Collaborators?
Vanderbilt
Our team worked closely with Vanderbilt University’s iGEM team over the summer to help them troubleshoot issues they had been having regarding a promoter and to come up with questions to ask our panel. Read more here
Help Us Out?
Washington University in St. Louis genetic engineering panel
Hey iGEM teams!
Here at Wash U we’re planning a panel on genetic engineering to be held in the fall for the Wash U community. We’re hoping to address some gray areas of synthetic biology and genetic engineering in agriculture, from safety and regulation to ethics and implications. We’re still finalizing our panelists, but hope to have experts from Monsanto and the Danforth Plant Science Center. We’re also in touch with some Wash U professors from the biology, philosophy, and anthropology departments.
We plan to start off the discussion with our own questions before opening it up to the audience. We’ve come up with a list of questions, but would love to get the input of some other iGEM teams. If you have questions you think would start interesting discussion at the panel, we’d love to hear them! Email us at
Our Amazing P.I's
The people that made it all possible
Tae Seok Moon
Faculty Advisor - Washington University
Fuzhong Zhang
Faculty Advisor - Washington University
Costas Maranas
Faculty Advisor - Pennsylvania State University
Cheryl Immethun
Washington University iGEM Advisor
Thomas Mueller
Penn State iGEM Advisor
Andrea Balassy
Washington University iGEM Advisor
Carlos Barba
Washington University iGEM Advisor
Yi Xiao
Washington University iGEM Advisor
Ray Henson
Washington University iGEM Advisor
Young Je Lee
Washington University iGEM Advisor