Difference between revisions of "Team:WashU StLouis"
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<div class="container"> | <div class="container"> | ||
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− | <!-- | + | <!-- Materials and Methods --> |
<div class="col-md-4 col-sm-6 overview-item"> | <div class="col-md-4 col-sm-6 overview-item"> | ||
<a href="#overviewModal5" class="overview-link" data-toggle="modal"> | <a href="#overviewModal5" class="overview-link" data-toggle="modal"> | ||
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</a> | </a> | ||
<div class="overview-caption"> | <div class="overview-caption"> | ||
− | <h4> | + | <h4>Materials and Methods</h4> |
<p class="text-muted">Imitation is the sincerest form of flattery.</p> | <p class="text-muted">Imitation is the sincerest form of flattery.</p> | ||
</div> | </div> | ||
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<div class="timeline-panel"> | <div class="timeline-panel"> | ||
<div class="timeline-heading"> | <div class="timeline-heading"> | ||
− | <h4>Blake | + | <h4>Blake Actkinson</h4> |
<h4 class="subheading">Biomedical Engineering</h4> | <h4 class="subheading">Biomedical Engineering</h4> | ||
</div> | </div> | ||
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</div> | </div> | ||
</div> | </div> | ||
+ | |||
</li> | </li> | ||
+ | <li class="row "> | ||
+ | <div class="row timeline-image"> | ||
+ | <img class="img-circle img-responsive" src="https://static.igem.org/mediawiki/2015/4/4b/Caroline_focht.jpg" alt=""> | ||
+ | </div> | ||
+ | <div class="timeline-panel"> | ||
+ | <div class="timeline-heading"> | ||
+ | <h4>Caroline Focht</h4><span class="text-muted">- 2014 iGEM Team Member</span> | ||
+ | <h4 class="subheading">Chemistry and Biochemistry</h4> | ||
+ | </div> | ||
+ | <div class="timeline-body"> | ||
+ | <p class="text-muted">Washington University in St. Louis</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </li> | ||
<li class="row"> | <li class="row"> | ||
<div class=" col-lg-12" style="text-align:center;" > | <div class=" col-lg-12" style="text-align:center;" > | ||
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<img height="255" width="255" src="https://static.igem.org/mediawiki/2015/2/28/Moon_72.jpg" class="img-responsive img-circle" alt=""> | <img height="255" width="255" src="https://static.igem.org/mediawiki/2015/2/28/Moon_72.jpg" class="img-responsive img-circle" alt=""> | ||
<h4>Tae Seok Moon</h4> | <h4>Tae Seok Moon</h4> | ||
− | <p class="text-muted">Faculty | + | <p class="text-muted">Faculty Advisor - Washington University</p> |
<ul class="list-inline social-buttons"> | <ul class="list-inline social-buttons"> | ||
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<img height="255" width="255" src="https://static.igem.org/mediawiki/2015/3/3f/FZhang.png" class="img-responsive img-circle" alt=""> | <img height="255" width="255" src="https://static.igem.org/mediawiki/2015/3/3f/FZhang.png" class="img-responsive img-circle" alt=""> | ||
<h4>Fuzhong Zhang</h4> | <h4>Fuzhong Zhang</h4> | ||
− | <p class="text-muted">Faculty Advisor</p> | + | <p class="text-muted">Faculty Advisor - Washington University</p> |
<ul class="list-inline social-buttons"> | <ul class="list-inline social-buttons"> | ||
<li><a href="http://zhang.eece.wustl.edu/"><i class="fa fa-mail-forward"></i></a> | <li><a href="http://zhang.eece.wustl.edu/"><i class="fa fa-mail-forward"></i></a> | ||
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<img height="255" width="255" src="https://static.igem.org/mediawiki/2015/4/4b/Cmaranas.jpg" class="img-responsive img-circle" alt=""> | <img height="255" width="255" src="https://static.igem.org/mediawiki/2015/4/4b/Cmaranas.jpg" class="img-responsive img-circle" alt=""> | ||
<h4>Costas Maranas</h4> | <h4>Costas Maranas</h4> | ||
− | <p class="text-muted">Faculty Advisor</p> | + | <p class="text-muted">Faculty Advisor - Pennsylvania State University</p> |
<ul class="list-inline social-buttons"> | <ul class="list-inline social-buttons"> | ||
<li><a href="http://maranasgroup.com/index.htm"><i class="fa fa-mail-forward"></i></a> | <li><a href="http://maranasgroup.com/index.htm"><i class="fa fa-mail-forward"></i></a> | ||
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<hr> | <hr> | ||
<div class="row"> | <div class="row"> | ||
− | <div class="col-sm-4"> | + | <div class="col-sm-4 col-xs-6"> |
<div class="team-member"> | <div class="team-member"> | ||
<img height="255" width="255" src="https://static.igem.org/mediawiki/2015/0/0b/Cheryl_72.jpg" class="img-responsive img-circle" alt=""> | <img height="255" width="255" src="https://static.igem.org/mediawiki/2015/0/0b/Cheryl_72.jpg" class="img-responsive img-circle" alt=""> | ||
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</div> | </div> | ||
</div> | </div> | ||
− | <div class="col-sm-4"> | + | <div class="col-sm-4 col-xs-6"> |
<div class="team-member"> | <div class="team-member"> | ||
<img height="255" width="255" src="https://static.igem.org/mediawiki/2015/f/f2/Thomas.jpg" class="img-responsive img-circle" alt=""> | <img height="255" width="255" src="https://static.igem.org/mediawiki/2015/f/f2/Thomas.jpg" class="img-responsive img-circle" alt=""> | ||
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</div> | </div> | ||
</div> | </div> | ||
− | + | ||
+ | <div class="col-sm-4 col-xs-6"> | ||
+ | <div class="team-member"> | ||
+ | <img height="255" width="255" src="https://static.igem.org/mediawiki/2015/c/cb/Andi.jpg" class="img-responsive img-circle" alt=""> | ||
+ | <h4>Andrea Balassy</h4> | ||
+ | <p class="text-muted">Washington University iGEM Advisor</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-sm-4 col-xs-6"> | ||
+ | <div class="team-member"> | ||
+ | <img height="255" width="255" src="https://static.igem.org/mediawiki/2015/f/fe/Carlos.png" class="img-responsive img-circle" alt=""> | ||
+ | <h4>Carlos Barba</h4> | ||
+ | <p class="text-muted">Washington University iGEM Advisor</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-sm-4 col-xs-6"> | ||
+ | <div class="team-member"> | ||
+ | <img height="255" width="255" src="https://static.igem.org/mediawiki/2015/b/b3/YiXiao.png" class="img-responsive img-circle" alt=""> | ||
+ | <h4>Yi Xiao</h4> | ||
+ | <p class="text-muted">Washington University iGEM Advisor</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-sm-4 col-xs-6"> | ||
+ | <div class="team-member"> | ||
+ | <img height="255" width="255" src="https://static.igem.org/mediawiki/2015/d/d0/Ray.jpg" class="img-responsive img-circle" alt=""> | ||
+ | <h4>Ray Henson</h4> | ||
+ | <p class="text-muted">Washington University iGEM Advisor</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-sm-4 col-xs-6"> | ||
+ | <div class="team-member"> | ||
+ | <img height="255" width="255" src="https://static.igem.org/mediawiki/2015/9/9c/Ryan_photo_small.jpg" class="img-responsive img-circle" alt=""> | ||
+ | <h4>Young Je Lee</h4> | ||
+ | <p class="text-muted">Washington University iGEM Advisor</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
<div class="row"> | <div class="row"> | ||
<div class="col-lg-8 col-lg-offset-2 text-center"> | <div class="col-lg-8 col-lg-offset-2 text-center"> | ||
− | <p class="large text-muted"> | + | <p class="large text-muted"></p> |
</div> | </div> | ||
</div> | </div> | ||
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<h2>2014 iGEM Project</h2> | <h2>2014 iGEM Project</h2> | ||
<p class="item-intro text-muted">Fixing Nitrogen and Sensing Light</p> | <p class="item-intro text-muted">Fixing Nitrogen and Sensing Light</p> | ||
− | <img class="img-responsive img-centered" src=" | + | <img class="img-responsive img-centered" src="https://static.igem.org/mediawiki/2014/4/4e/WashU_Project_Overview.png" alt=""> |
<h4> What was our goal?</h4> | <h4> What was our goal?</h4> | ||
<p>There were two key components of our project: </p> | <p>There were two key components of our project: </p> | ||
− | <p>Richard and Caroline were working in the <a href="https://pages.wustl.edu/photo.synth.bio">Pakrasi </a> lab, and had been using genes from cyanobacteria to get nitrogen fixation working in <em> E. coli</em>. They were testing nitrogen fixation by running acetylene reduction assays and designing experiments to test the optimal criteria (ie. <em> E. coli</em> strains, temperature | + | <p>Richard and Caroline were working in the <a href="https://pages.wustl.edu/photo.synth.bio">Pakrasi </a>lab, and had been using genes from cyanobacteria to get nitrogen fixation working in <em>E. coli</em>. They were testing nitrogen fixation by running acetylene reduction assays and designing experiments to test the optimal criteria (ie. <em>E. coli</em> strains, temperature, nitrogen source) to get maximal results. |
</p> | </p> | ||
<strong> Read more <a href="https://2014.igem.org/Team:WashU_StLouis/Project/nif"> here </a> </strong> | <strong> Read more <a href="https://2014.igem.org/Team:WashU_StLouis/Project/nif"> here </a> </strong> | ||
− | <p>Ben and Jeffery | + | <p>Ben and Jeffery worked in the <a href="http://moon.eece.wustl.edu/"> Moon </a> lab, and cloned plasmids to create a system that represses and expresses a fluorescent protein with the presence and absence of light, and were running experiments to test the induction levels compared to various positive and negative controls.</p> |
<strong> Read more <a href="https://2014.igem.org/Team:WashU_StLouis/Project/light"> here </a> </strong> | <strong> Read more <a href="https://2014.igem.org/Team:WashU_StLouis/Project/light"> here </a> </strong> | ||
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</video> | </video> | ||
</div> | </div> | ||
− | <p> The goal of this year's project is to take the nitrogen fixing nif cluster from | + | <span>Caroline Focht</span><p class="text-muted">- 2014 Washington University iGEM member</p> |
+ | <a href="https://www.youtube.com/watch?v=5B73_8vcENI&list=PL6fJjAsbg4Sz1V-Xu0moedDxG9Hbbqx8N">More Videos!!!</a> | ||
+ | <p> The goal of this year's project is to take the nitrogen fixing <em>nif</em> cluster from <em>Cyanothece</em>, a cyanobacterium, minify it, and make it work in <em>E. coli</em>. </p> | ||
<ul style="text-align:left;"> | <ul style="text-align:left;"> | ||
− | <li><a href="#">Designing the plasmid </a> | + | <li><a href="#">Designing the plasmid TODO ADD LINkS</a> |
− | <li><a href="#">Making it work in E. Coli</a> | + | <li><a href="#">Making it work in E. Coli TODO ADD LINKS</a> |
</ul> | </ul> | ||
− | <button type="button" class="btn btn-primary" data-dismiss="modal"><i class="fa fa-times"></i> Close Modal</button> | + | <button type="button" class="btn btn-primary" data-dismiss="modal"><i class="fa fa-times"></i>Close Modal</button> |
</div> | </div> | ||
</div> | </div> | ||
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<h2>The Big Picture</h2> | <h2>The Big Picture</h2> | ||
<p class="item-intro text-muted">Haber-Bosch is so 1909</p> | <p class="item-intro text-muted">Haber-Bosch is so 1909</p> | ||
− | <img class="img-responsive img-centered" src=" | + | <img class="img-responsive img-centered" src="https://static.igem.org/mediawiki/2015/6/65/Fertilizer-Manufacturing-Important-for-the-Country.jpg" alt=""> |
<h3>Why are we doing this project</h3> | <h3>Why are we doing this project</h3> | ||
− | Synthetic biology is an exciting area of research that aims to genetically improve organisms to make them more efficient and hopefully more useful to us as well. The human population in 1950 was 2.5 billion, yet it is predicted to surpass 9 billion by 2050. Even with population growth slowing, increasing life spans and standards of living will soon tax our natural resources. One of the most concerning is our food supply. The agriculture industry needs a revolution in order to keep up with our expected growth rates. Currently about 80% of chemically fixated nitrogen is used as agricultural fertilizers, the majority in developed lands . Intracellular nitrogen fixation in crops could help to sustain the burgeoning world population, especially in areas with less fertile soil without taxing the planet’s waterways. The exponential increase in nitrogen fertilizer has led to more runoff into rivers and oceans. Fertilizers then provide nutrition for algal blooms that result in hypoxia and form oceanic dead zones. These dead zones lead to the death of marine species and have potentially large economic consequences. | + | Synthetic biology is an exciting area of research that aims to genetically improve organisms to make them more efficient and hopefully more useful to us as well. The human population in 1950 was 2.5 billion, yet it is predicted to surpass 9 billion by 2050 <a href="#" data-container="body" data-toggle="popover" data-placement="top" data-content="Rogers, Oldroyd. Journal of Experimental Botany March 2014 doi:10.1093/jxb/eru098" ><sup>[1]</sup></a>". Even with population growth slowing, increasing life spans and standards of living will soon tax our natural resources. One of the most concerning is our food supply. The agriculture industry needs a revolution in order to keep up with our expected growth rates. Currently about 80% of chemically fixated nitrogen is used as agricultural fertilizers, the majority in developed lands<a href="#" data-container="body" data-toggle="popover" data-placement="top" data-content="Rogers, Oldroyd. Journal of Experimental Botany March 2014 doi:10.1093/jxb/eru098" ><sup>[1]</sup></a>. Intracellular nitrogen fixation in crops could help to sustain the burgeoning world population, especially in areas with less fertile soil without taxing the planet’s waterways. The exponential increase in nitrogen fertilizer has led to more runoff into rivers and oceans. Fertilizers then provide nutrition for algal blooms that result in hypoxia and form oceanic dead zones. These dead zones lead to the death of marine species and have potentially large economic consequences. |
The ramifications of nitrogen fertilizer runoff can be averted by genetically engineering plant crops to fix their own nitrogen. Some cyanobacteria fix nitrogen for nutritional needs, while most organisms can only acquire it from the food it consumes. Synthetic biology allows us to transfer this ability to fix nitrogen to a heterologous host that has many genetic tools, Escherichia coli, so that we can learn how to give single cell organisms, and eventually chloroplasts the ability to create their own nitrogen fertilizer. | The ramifications of nitrogen fertilizer runoff can be averted by genetically engineering plant crops to fix their own nitrogen. Some cyanobacteria fix nitrogen for nutritional needs, while most organisms can only acquire it from the food it consumes. Synthetic biology allows us to transfer this ability to fix nitrogen to a heterologous host that has many genetic tools, Escherichia coli, so that we can learn how to give single cell organisms, and eventually chloroplasts the ability to create their own nitrogen fertilizer. | ||
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<div class="embed-responsive embed-responsive-16by9"> | <div class="embed-responsive embed-responsive-16by9"> | ||
− | + | </div> | |
− | + | ||
− | + | ||
− | + | ||
<button type="button" class="btn btn-primary" data-dismiss="modal"><i class="fa fa-times"></i> Close Modal</button> | <button type="button" class="btn btn-primary" data-dismiss="modal"><i class="fa fa-times"></i> Close Modal</button> | ||
</div> | </div> | ||
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<div class="modal-body"> | <div class="modal-body"> | ||
<!-- Project Details Go Here --> | <!-- Project Details Go Here --> | ||
− | <h2> | + | <h2>Materials and Methods</h2> |
<p class="item-intro text-muted">How we did what we did</p> | <p class="item-intro text-muted">How we did what we did</p> | ||
− | <img class="img-responsive img-centered" src=" | + | <img class="img-responsive img-centered" src="https://static.igem.org/mediawiki/2014/7/7c/WashU_Engineered_Strains.jpg" alt=""> |
TODO impement this page | TODO impement this page | ||
<button type="button" class="btn btn-primary" data-dismiss="modal"><i class="fa fa-times"></i> Close Modal</button> | <button type="button" class="btn btn-primary" data-dismiss="modal"><i class="fa fa-times"></i> Close Modal</button> | ||
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<h2>Discussion</h2> | <h2>Discussion</h2> | ||
<p class="item-intro text-muted">Where do we go from here</p> | <p class="item-intro text-muted">Where do we go from here</p> | ||
− | <img class="img-responsive img-centered" src="http:// | + | <a href="https://xkcd.com/1501/"> |
+ | <img class="img-responsive img-centered" src="http://imgs.xkcd.com/comics/mysteries.png" alt=""> | ||
+ | <p class="muted">XKCD</p> | ||
+ | </a> | ||
TODO implement this | TODO implement this | ||
<button type="button" class="btn btn-primary" data-dismiss="modal"><i class="fa fa-times"></i> Close Modal</button> | <button type="button" class="btn btn-primary" data-dismiss="modal"><i class="fa fa-times"></i> Close Modal</button> |
Revision as of 00:36, 4 August 2015
Welcome To Our Website!
WashU and Penn State iGEM 2015
Project Description
Let's Talk
Apply for the 2016 iGEM Team!
Team
NitroGeniuses
-
Jessica O'Callaghan
Chemical Engineering
Penn State
-
Laura Beebe
Biochemistry and Molecular Biology
Penn State
-
Blake Actkinson
Biomedical Engineering
Washington University in St. Louis
-
Charlotte Bourg
Biochemistry and Spanish
Washington University in St. Louis
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David Ayeke
Computer Engineering
Washington University in St. Louis
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Mike Toomey
Biomedical Engineering
Washington University in St. Louis
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Caroline Focht
- 2014 iGEM Team MemberChemistry and Biochemistry
Washington University in St. Louis
Collaboration
:{ Looking for Collaborators
Vanderbilt
Our Amazing P.I's
The people that made it all possible
Tae Seok Moon
Faculty Advisor - Washington University
Fuzhong Zhang
Faculty Advisor - Washington University
Costas Maranas
Faculty Advisor - Pennsylvania State University
Cheryl Immethun
Washington University iGEM Advisor
Thomas Mueller
Penn State iGEM Advisor
Andrea Balassy
Washington University iGEM Advisor
Carlos Barba
Washington University iGEM Advisor
Yi Xiao
Washington University iGEM Advisor
Ray Henson
Washington University iGEM Advisor
Young Je Lee
Washington University iGEM Advisor
Contact Us
We can't do this alone
This form will open your default mail client (i.e Outlook). If you do not have one installed, email us at WashU iGEM washu.igem@gmail.com