Difference between revisions of "Team:WashU StLouis/Safety"

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<h2>Safety in iGEM</h2>
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        <a class="navbar-brand page-scroll" href="#page-top">Nitrogenius</a>
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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            <a class="page-scroll" href="#safety">Safety</a>
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            <a href="mailto:ayekedavidr@wustl.edu?subject=Bug">Report Bugs</a>
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        <h1>Safety</h1>
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        <p>Our labs are at Risk Level 1, meaning all reagents used in lab are well characterized and known not to cause disease within humans. These labs are equipped with safety showers, eye washes, and fire extinguishers if any emergency were to occur. All members have gone through extensive training to work in these labs, which included a detailed tour of facilities and numerous quizzes over safety standards. No member was allowed to be in lab alone unless under supervision of a graduate student. </p>
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        <p>Our labs used the following E. coli strains: DH10B, BL21, MG1655, JM109, and WM1788. All strains are Group I organisms, which have been proven not to cause disease in humans. In working with these organisms, we used fume hoods and ensured no contamination occurred through bleaching any area these organisms come into contact with. Gloves were worn at all times while in lab. Group members disposed reagents used in experiments into their respective waste bins for proper disposal. </p>
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        <p>We wanted to develop nitrogen fixation within E. coli to understand how this biochemical process could be translated to plants. To date, hazardous effects of introducing these genes into E. coli have not been observed. </p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h4>Safe Project Design</h4>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h4>Safe Lab Work</h4>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h4>Safe Shipment</h4>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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{{:Team:WashU_StLouis/Footer}}

Latest revision as of 00:41, 18 September 2015

Washington University - Penn State iGEM

Welcome To Our Website!
WashU and Penn State   iGEM 2015
Project Description Let's Talk Apply for the 2016 iGEM Team!

Safety

Our labs are at Risk Level 1, meaning all reagents used in lab are well characterized and known not to cause disease within humans. These labs are equipped with safety showers, eye washes, and fire extinguishers if any emergency were to occur. All members have gone through extensive training to work in these labs, which included a detailed tour of facilities and numerous quizzes over safety standards. No member was allowed to be in lab alone unless under supervision of a graduate student.

Our labs used the following E. coli strains: DH10B, BL21, MG1655, JM109, and WM1788. All strains are Group I organisms, which have been proven not to cause disease in humans. In working with these organisms, we used fume hoods and ensured no contamination occurred through bleaching any area these organisms come into contact with. Gloves were worn at all times while in lab. Group members disposed reagents used in experiments into their respective waste bins for proper disposal.

We wanted to develop nitrogen fixation within E. coli to understand how this biochemical process could be translated to plants. To date, hazardous effects of introducing these genes into E. coli have not been observed.