Difference between revisions of "Team:William and Mary/Description"
Line 161: | Line 161: | ||
<div><p style="float: left;"><IMG SRC="https://static.igem.org/mediawiki/2015/7/74/WMraser.png"></p><p> Panel A shows an example of intrinsic and extrinsic noise; both the ratio of the fluorescence of the two reporters (intrinsic) and the absolute fluorescence of the two reporters (extrinsic) changes. Panel B shows an example of extrinsic noise only; the ratio of the fluorescence of the two reporters stays constant while the absolute fluorescence changes. Figure taken from:[2].</p></div></p> | <div><p style="float: left;"><IMG SRC="https://static.igem.org/mediawiki/2015/7/74/WMraser.png"></p><p> Panel A shows an example of intrinsic and extrinsic noise; both the ratio of the fluorescence of the two reporters (intrinsic) and the absolute fluorescence of the two reporters (extrinsic) changes. Panel B shows an example of extrinsic noise only; the ratio of the fluorescence of the two reporters stays constant while the absolute fluorescence changes. Figure taken from:[2].</p></div></p> | ||
+ | <div><p>Once individual cell measurements have been taken for both CFP and YFP expression using confocal microscopy, the intrinsic noise measurements can be calculated (link to John’s part about this) and further analyzed.</p></div> | ||
− | <div><p style="float: | + | <div><p style="float: right;"><IMG SRC="https://static.igem.org/mediawiki/2015/9/97/WMdCasDiag.jpeg"></p><p>In addition to characterizing the noise of promoters in iGEM, we contributed additional tools for manipulating their expression. CRISPR/Cas9 has been used extensively in synthetic biology, both inside and out of iGEM. In particular, new functionalizations of the Cas9 protein that remove its catalytic nuclease domain while retaining its DNA-binding activity have allowed for novel methods in molecular biology. This catalytically inactive variant of Cas9, known as dCas9, can be used to repress gene expression by targeting the promoter region of a gene of interest. This repression is mediated by the CRISPR/Cas9 complex binding to the promoter region and can block RNAP binding or prevent transcriptional elongation. All of our gRNAs prevent RNAP binding and initiation of transcription.</p></div></p> |
− | + | ||
− | + | ||
<p> | <p> |
Revision as of 11:43, 18 September 2015
Integrator Suites
Detail1
Detail2
Detail3
Detail4
text
text
Antibiotic Operons
Detail1
Detail2
Detail3
Detail4
text
text
dCas9s
Detail1
Detail2
Detail3
Detail4
text
text
gRNAs
Detail1
Detail2
Detail3
Detail4
text
text
XFPs Under Various Promoters
Detail1
Detail2
Detail3
Detail4
text
text
G^2
Detail1
Detail2
Detail3
Detail4
text
text