Difference between revisions of "Team:William and Mary/Parts"
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| − | <p> | + | <p>This plasmid (http://parts.igem.org/Part:BBa_K1795022) consists of both a CFP-LVA driven by R0010 and YFP-LVA driven by R0010. This allows future teams to use this part to investigate transcriptional noise either on a low copy number plasmid or by integrating this part using our galK integrator (http://parts.igem.org/Part:BBa_K1795023). </p> |
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Revision as of 12:17, 18 September 2015
All Parts (By Category) In deciding which parts to submit to the iGEM Registry we focused on three main aspects. First: ensuring our project is as reproducible and extensible as possible. To that end we have submitted all of new composite fluorescent protein parts that we constructed during the project. Second: Making genome integration as straightforward as possible for iGEM teams. In order to accomplish this goal we designed, tested, and validated a new integrator cassette that allows for simple genome integration using either 3A or Gibson Assembly. Third: Increasing the number of tools available for promoter-mediated regulation in synthetic biology. We created and validated an E. coli codon optimized dCas9 variant and a suite of gRNAs to target the most commonly used promoters in iGEM. Integrator Suites Detail1 Detail2 Detail3 Detail4 text text Antibiotic Operons Detail1 Detail2 Detail3 Detail4 text text dCas9s Detail1 Detail2 Detail3 Detail4 text text gRNAs Detail1 Detail2 Detail3 Detail4 text text XFPs Under Various Promoters Detail1 Detail2 Detail3 Detail4 text text G^2 This plasmid (http://parts.igem.org/Part:BBa_K1795022) consists of both a CFP-LVA driven by R0010 and YFP-LVA driven by R0010. This allows future teams to use this part to investigate transcriptional noise either on a low copy number plasmid or by integrating this part using our galK integrator (http://parts.igem.org/Part:BBa_K1795023).
